Characterization of a tomato chlh mis‐sense mutant reveals a new function of ChlH in fruit ripening DOI Creative Commons
Dawei Xu, Li Lin, Xiaorui Liu

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Tomato fruit ripening is a complex developmental process that important for quality and shelf life. Many factors, including ethylene several key transcription have been shown to play roles in the regulation of tomato ripening. However, our understanding still limited. Here, we describe mut26, an EMS-induced (Solanum lycopersicum) mutant exhibits chlorophyll-deficient phenotypes various organs, fruits. Genetic mapping functional analyses revealed single-nucleotide substitution corresponding Pro398->Ser mis-sense mutation SlChlH (GENOMES UNCOUPLED 5, GUN5), which encodes H subunit magnesium chelatase, are responsible defects mut26 strain. Transcript towards expression many SlPhANGs defective plastid retrograde signalling during initiation, namely transition from mature green breaker stage. delayed progression characterized by reduced emission, increased firmness, carotenoid content conversion chloroplast chromoplast. Given requires plastids nucleus, these data support hypothesis GUN5-mediated promotes We further showed not likely caused chlorophyll content. Taken together, identified new function promotion biosynthesis, suggesting plays promotive role

Language: Английский

Removal of toxic steroidal glycoalkaloids and bitterness in tomato is controlled by a complex epigenetic and genetic network DOI Creative Commons

Feng Bai,

Mengbo Wu, Wei Huang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(8)

Published: Feb. 19, 2025

The steroidal glycoalkaloids (SGAs) produced in Solanaceae crops, including tomato, are antinutritional because of their cellular toxicity and resultant bitter taste to humans. To make fruits palatable, SGA profiles shift from toxic α-tomatine nonbitter nontoxic esculeoside A during the ripening process. However, mechanisms regulating this conversion remain unclear. In study, we showed that removal SGAs is under control DNA demethylation, ethylene, key transcription factors by forming a feedback loop governs expression GLYCOALKALOID METABOLISM ( GAME ) genes ripening. Moreover, ethylene-inducible NON-RIPENING, RIPENING INHIBITOR, FRUITFULL1 coordinately regulate GAME31 , GAME40 GAME5 glycoalkaloid transporter gene GORKY whereas jasmonic acid–induced MYC2 modulates GAME36 . Furthermore, demethylation mediated DEMETER - LIKE 2 drives detoxification tomato domestication.

Language: Английский

Citations

3
Transcription factors SlNOR and SlNOR-like1 regulate steroidal glycoalkaloids biosynthesis in tomato fruit DOI

Lan-Ting Xiang,

Hongli Li,

Jian-Lin He

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 299, P. 140157 - 140157

Published: Jan. 21, 2025

Language: Английский

Citations

1
Two transcription factors play critical roles in mediating epigenetic regulation of fruit ripening in tomato DOI Creative Commons
Qingfeng Niu, Yaping Xu, Huan Huang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(15)

Published: April 9, 2025

DNA methylation regulates fruit ripening in tomato, and disruption of the demethylase DEMETER-LIKE 2 (DML2) results genome-wide hypermethylation impaired ripening. We report here that transcription factors Ripening Inhibitor (RIN) FRUITFULL 1 (FUL1) play critical roles mediating effect on tomato RIN FUL1 are silenced dml2 mutant plants, defective phenotype is mimicked by rin/ful1 double mutant. Restoration expression partially rescues its defects. controls not only regulating but also interfering with genomic binding RIN. In cannot bind to some targets vivo even though does interfere vitro; this inhibited correlated increased histone H3 enrichment within 100 bp site. Our work uncovers molecular mechanisms underlying control tomato.

Language: Английский

Citations

1
Strategies to Delay Ethylene-Mediated Ripening in Climacteric Fruits: Implications for Shelf Life Extension and Postharvest Quality DOI Creative Commons
Ramiro Alonso-Salinas, Santiago López‐Miranda, Antonio J. Pérez-López

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 840 - 840

Published: Aug. 8, 2024

Climacteric fruits undergo a characteristic ripening process regulated by ethylene, key plant hormone. Extending the shelf life of these while preserving their postharvest quality poses significant challenge for food industry. This review provides comprehensive overview physiological and molecular strategies to delay ethylene-mediated in climacteric impact on life, quality, sensory attributes, volatile compounds. Additionally, it examines role ethylene fruit ripening, analysing various managing including inhibitors, adsorbents, scavengers catalytic oxidation. concludes with future research directions genetic approaches reducing production or responsiveness fruits, integrated strategies, environmental considerations, commercial applications improving handling quality.

Language: Английский

Citations

4
Transcription factor AcAGL9 and AcBEH1 synergistically regulate carotenoid biosynthesis during pineapple fruit ripening DOI
Mengting Liu, Ting Li,

Rufang Deng

et al.

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 222, P. 113385 - 113385

Published: Jan. 9, 2025

Language: Английский

Citations

0
American ginseng fruit: Antioxidant capacity, bioactive components, and biosynthesis mechanism during development DOI
Yujuan Zhang,

Lijuan Hou,

Jing Hu

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115884 - 115884

Published: Jan. 30, 2025

Language: Английский

Citations

0
lncRNA1471 mediates tomato‐ripening initiation by binding to the ASR transcription factor DOI Open Access
Lingling Zhang, Guoning Zhu, Liqun Ma

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

The regulatory mechanisms underlying fruit ripening, including hormone regulation, transcription factor activity, and epigenetic modifications, have been discussed extensively. Nonetheless, the role of long non-coding RNAs (lncRNAs) in ripening remains unclear. Here, we identified lncRNA1471 as a negative regulator tomato fruit-ripening initiation. Knocking out via large fragment deletion resulted accelerated initiation shorter color-breaking stage (BR), deeper coloration, increased levels ethylene, lycopene, β-carotene, chlorophyll degradation, reduced firmness. These phenotypic changes were accompanied by alterations carotenoid pathway flux, ethylene biosynthesis, cell wall metabolism, primarily mediated direct regulation key genes involved these processes. For example, CR-lncRNA1471 mutant, lycopene-related SlPSY1 SlZISO upregulated. Additionally, expression biosynthetic (SlACS2 SlACS4), ripening-related (RIN, NOR, CNR, SlDML2), metabolism (SlPL, SlPG2a, SlEXP1, SlPMEI-like, SlBG4) significantly upregulated, which further strengthening findings mentioned above. Furthermore, was to interact with abscisic stress-ripening protein (ASR) chromatin isolation RNA purification coupled mass spectrometry (ChIRP-MS) pull-down assay vitro, might regulate ripening. discovery significant enhances our understanding complex landscape governing provide valuable insights into particularly regarding involvement lncRNAs

Language: Английский

Citations

0
p-chlorophenylalanine treatment accelerates tomato fruit ripening through hormone synthesis and glycolytic pathway during storage DOI
Kailin Xu,

Ran Yan,

Hang Zhang

et al.

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 225, P. 113517 - 113517

Published: March 17, 2025

Language: Английский

Citations

0
Genetic variation in a tandemly duplicated TPS gene cluster contributes to the diversity of aroma in lychee fruit DOI Creative Commons
Huimin Hu,

Hongsen Liu,

Zaohai Zeng

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Fruits undergo a similar ripening process, yet they exhibit range of differences in color, taste, and shape, both across different species within the same species. How does this diversity arise? We uncovered conserved fruit process lychee which NAC transcription factor, LcNAC1, acts as master regulator. LcNAC1 regulates expression two terpene synthase genes, LcTPSa1 LcTPSa2, belong to gene cluster consisting four TPS genes. LcTPSa1-LcTPSa3 are responsible for catalyzing production farnesol, turn dictates aromatic varieties. Through comparative, transcriptomic, genomic analyses various varieties, we found these genes distinct levels due natural genetic variation. These include copy number variations, presence/absence insertions deletions, single nucleotide polymorphisms, many affect binding affinity LcNAC1. A mutation caused premature translational termination, resulting truncated version protein, surprisingly remains functional. All changes LcNAC1-regulated likely contribute great observed fruit. This diversification aroma varieties offers compelling example how species- or variety-specific traits evolve - phenotypic is primarily derived from variation accumulated downstream structural an evolutionarily regulatory circuit.

Language: Английский

Citations

0
Transcription factor ZbERF6-ZbbHLH2 complex enhances monoterpenoid synthesis in Zanthoxylum bungeanum by synergistically activating the expression of ZbIDI DOI Creative Commons
Jie Zhang,

Nuan Han,

Ziyi Wang

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0