Advances in 3D printing combined with tissue engineering for nerve regeneration and repair

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 3, 2025

Language: Английский

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Plasma miRNAs as potential biomarkers for schizophrenia in a Jordanian cohort

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(2), P. 350 - 358

Published: Jan. 30, 2024

Schizophrenia (SZ), a complex and chronic neuropsychiatric disorder affecting approximately 1 % of the general population, presents diagnostic challenges due to absence reliable biomarkers, relying mainly on clinical observations. MicroRNAs (miRNAs) signatures in wide range diseases, including psychiatric disorders, hold immense potential for serving as biomarkers. This study aimed analyze expression levels specific microRNAs namely miR-29b-3p, miR-106b-5p, miR-199a-3p explore their SZ Jordanian patients. Small RNAs were extracted from plasma samples 30 patients 35 healthy controls. RNA was reverse transcribed quantified by real-time polymerase chain reaction (qRT-PCR). The three miRNAs (miR-29b-3p, miR-106b-5p miR-199a-3p) analyzed. Receiver operating characteristic (ROC) curves analysis performed evaluate value these miRNAs. Target genes prediction, functional enrichment pathway analyses done using miRWalk Metascape. STRING database used construct protein-protein network identify hub genes. Notably, significantly upregulated (p < 0.0001), while miRNA-29b-3p downregulated 0.0001) compared assessed through ROC curves, revealing substantial (AUC: 0.979) followed 0.774), with limited efficacy miR-29b-3p. Additionally, bioinformatic predicted target diagnostically significant uncovered Gene Ontology (GO) terms related neurological development, morphogenesis, which is involved neuron differentiation, brain head projection morphogenesis. These findings highlight connection between identified pathophysiology studied population. Furthermore, interaction association development deepens our comprehension molecular landscape regulated comprehensive exploration highlights promising role unraveling intricate pathways associated cohort suggests that could serve biomarkers diagnosis disease progression. Remarkably, this represents first investigation into circulating miRNA among SZ, providing valuable insights disorder.

Language: Английский

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Stem cell therapy for the treatment of psychiatric disorders: a real hope for the next decades

Frontiers in Psychiatry, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

In this review, it is evaluated the progress in application of stem cell therapy to ameliorate symptoms bipolar disorder, major depression, schizophrenia, and autism. These disorders are highly prevalent clinical medicine responsible for high levels psychosocial disability among patients. All them share common biomedical features, such as complex variable genetic substrates, significant susceptibility environmental changes, insufficient knowledge their pathogenesis. addition, responsiveness patients pharmacological treatment heterogeneous, some cases, no available. Therefore, development cell-based regenerative its possible combination with emerging therapeutic approaches that promote neural plasticity expected advance neuropsychiatry next few decades.

Language: Английский

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Extracellular vesicles derived from mesenchymal stem cells alleviate depressive-like behavior in a rat model of chronic stress

Life Sciences, Journal Year: 2025, Volume and Issue: 366-367, P. 123479 - 123479

Published: Feb. 20, 2025

Language: Английский

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Olfactory mucosa mesenchymal stem cell‐derived exosomes protect against neuroinflammation after subarachnoid hemorrhage by activating mitophagy

The Kaohsiung Journal of Medical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract Subarachnoid hemorrhage (SAH) can lead to significant acute neuroinflammation, with treatment outcomes often being inadequate. Olfactory mucosa mesenchymal stem cells (OM‐MSCs) have promising therapeutic potential in nerve regeneration and functional recovery. This investigation sought elucidate the mechanisms through which exosomes derived from OM‐MSCs provide protection against neuroinflammation following SAH. Mouse their were isolated characterized using various techniques, including transmission electron microscopy, immunofluorescence staining, Western blotting, flow cytometry, nanoparticle tracking analysis. Hemin‐induced HT22 subsequently utilized assess impact of OM‐MSC‐derived on inflammatory response, apoptosis, mitophagy ELISAs, qPCR, staining. The impacts neuronal damage SAH model mice assessed TUNEL Exosomes had capacity reduce levels proinflammatory factors (IL‐6, IL‐1 β , TNF‐ α ) promote apoptosis hemin‐induced cells. alleviated injury post‐SAH, as evidenced by increase modified Garcia scores, reduction brain water content, decrease blood–brain barrier permeability, decreases marker levels, rates. Notably, protective effects both vitro vivo, mediated via activation mitophagy. These findings a fresh perspective for subsequent clinical research domain prevention strategies.

Language: Английский

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Unraveling the potential of neuroinflammation and autophagy in schizophrenia

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 997, P. 177469 - 177469

Published: March 5, 2025

Language: Английский

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Intranasal delivery of engineered extracellular vesicles promotes neurofunctional recovery in traumatic brain injury

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 21, 2025

Traumatic brain injury (TBI) is a leading cause of disability in adults, significantly affecting patients' quality life. Extracellular vesicles (EVs) derived from human adipose-derived mesenchymal stem cells (hADSCs) have demonstrated therapeutic potential TBI treatment. However, their limited targeting ability, short half-life, and low bioavailability present significant challenges for clinical application. In this study, we engineered extracellular (EEVs) by transfecting hADSCs with lentivirus incorporating ultra-small paramagnetic nanoparticles (USPNs), resulting EVs enhanced miRNA expression targeted delivery capabilities. These EEVs were administered intranasally to specifically target sites, effectively modulating the NF-κB signaling pathway suppress neuroinflammation. both vitro vivo assessments, exhibited superior efficacy promoting neurofunctional recovery neurogenesis after compared unmodified EVs. Furthermore, validation using organoid models confirmed EEVs' remarkable ability neuroinflammation, offering promising strategy

Language: Английский

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Extracellular vesicles as precision therapeutics for psychiatric conditions: targeting interactions among neuronal, glial, and immune networks

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 8, 2025

The critical role of the immune system in brain function and dysfunction is well recognized, yet development therapies for psychiatric diseases has been slow due to concerns about iatrogenic deficiencies. These are emphasized by lack objective diagnostic tools psychiatry. A promise resolve this conundrum lies exploitation extracellular vesicles (EVs) that physiologically produced or can be synthetized. EVs regulate recipient cell functions offer potential EVs-based therapies. Intranasal administration enables targeting specific regions functions, thereby facilitating design precise treatments diseases. such requires navigating four dynamically interacting networks: neuronal, glial, immune, EVs. networks profoundly influenced fluid distribution. They crucial homeostasis, cellular intercellular communication. Fluid abnormalities, like edema altered cerebrospinal (CSF) dynamics, disrupt these networks, negatively impacting health. deeper understanding above-mentioned vital creating biomarker panels identify distinct patient subsets with similar neuro-behavioral symptoms. Testing functional pathways biomarkers could lead new therapeutic tools. Regulatory approval will depend on robust preclinical data reflecting progress interdisciplinary areas, which pave way innovative treatments. Highly collaborative teams needed achieve ambitious goals.

Language: Английский

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The roles of extracellular vesicles in mental disorders: information carriers, biomarkers, therapeutic agents

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Mental disorders are complex conditions that encompass various symptoms and types, affecting approximately 1 in 8 people globally. They place a significant burden on both families society as whole. So far, the etiology of mental remains poorly understood, making diagnosis treatment particularly challenging. Extracellular vesicles (EVs) nanoscale particles produced by cells released into extracellular space. contain bioactive molecules including nucleotides, proteins, lipids, metabolites, which can mediate intercellular communication involved physiological pathological processes. Recent studies have shown EVs closely linked to like schizophrenia, major depressive disorder, bipolar playing key role their development, diagnosis, prognosis, treatment. Therefore, based recent research findings, this paper aims describe roles summarize potential applications treatment, providing new ideas for future clinical transformation application EVs.

Language: Английский

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Dysregulation of miRNAs in Schizophrenia in an Egyptian Patient Population

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Schizophrenia (SZ) is a complex neuropsychiatric disorder influenced by genetic, environmental, and epigenetic factors, including miRNA dysregulation. This study explored the diagnostic therapeutic potential of miRNAs in SZ, focusing on seven key miRNAs: miR-137-3p, miR-34a-5p, miR-432-5p, miR-130b-3p, miR-346, miR-195-5p, miR-103a-3p. Results revealed significant dysregulation miR-103a-3p, highlighting their relevance to SZ pathology. Upregulation miR-137-3p correlated with enhanced cognitive performance, as evidenced improved scores Wisconsin Card Sorting Test (WCST) Trail Making B (TMT-B). Conversely, miR-195-5p miR-346 were strongly associated processing speed, while miR-103a-3p downregulation was linked reduced conceptual flexibility. Cluster analyses demonstrated that expression levels varied significantly based antipsychotic treatment receptor targeting, suggesting regulatory effects medication. Importantly, measured PBMCs, feasibility non-invasive biomarkers. The underscores value miRNAs, offering promising avenue for early detection personalized interventions SZ. Future research should validate these findings across diverse cohorts investigate miRNA-based strategies. By integrating profiling into clinical practice, this provides foundation advancing precision medicine management.

Language: Английский

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