Dataset on transcriptomic profiling of parenteral nutrition-induced hepatotoxicity in a human liver in vitro model DOI Creative Commons
Milos Mihajlovic, Mathieu Vinken

Data in Brief, Journal Year: 2024, Volume and Issue: 55, P. 110653 - 110653

Published: June 18, 2024

The provided dataset describes the transcriptomic profile of human liver spheroid co-cultures consisting a hepatoma cell line (C3A/HepG2 cells) and an immortalized activated hepatic stellate (LX-2 upon exposure to total parenteral nutrition. High-throughput RNA sequencing was performed using DNBSEQTM technology. Following quality check filtering raw sequence reads, clean reads were aligned reference genome used determine differential gene expression. Raw processed data are deposited in Gene Expression Omnibus with accession number GSE264357. These could serve further mechanistic studies on nutrition-induced injury support translational research intestinal failure-associated disease occurring individuals receiving

Language: Английский

Development and Validation of In Vitro Assessment Protocol of Novel Intravenous Nanoemulsions for Parenteral Nutrition DOI Creative Commons
Joanna Czerniel, Aleksandra Gostyńska, Karina Sommerfeld

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 493 - 493

Published: April 8, 2025

Background: Parenteral nutrition (PN) is a lifesaving therapeutic approach for patients unable to meet nutritional needs through oral or enteral routes. Lipid nanoemulsions (NEs), critical component of PN, provide essential fatty acids and influence the formulation’s physicochemical properties. Advances in drug delivery systems have led novel intravenous NEs with improved stability, purity, ability nutrient/active substance delivery. Due scattered information lack standardized protocol testing lipid NEs, this study aimed develop robust assessment method. The focuses on characterizing properties, biological efficacy while adhering bioethical standards. Methods: Four were formulated based acid profile analysis, demonstrate applicability our protocol, each NE utilized distinct emulsifier system. A comprehensive vitro integrating multiple analytical techniques was employed evaluate their performance. Results: mean droplet diameter (MDD) developed ranged from 180.7 185.9 nm, significantly smaller than commercial formulations (249.6–335.4 nm). PFAT5 remained below 0.02%, except ILE-HS (0.12%), zeta potential (ZP) −29.8 mV. freeze–thaw stability constant (KF) range formulation, sterilization (KS) 10, (23.61 ± 1.65). Injectability tests confirmed that ILE-ELP ILE-T could be infused at 50 mL/h using an access minimum 21 G. Hemolytic activity met strictest criteria (<5%), MTT assays showed higher cell viability low concentrations all ILE-ELP. Conclusions: five-step provides unified framework assessing allowing selection highest further vivo assessment.

Language: Английский

Citations

1
Intestinal Microbiota Modulation by Fecal Microbiota Transplantation in Nonalcoholic Fatty Liver Disease DOI Creative Commons
Goran Hauser, Indira Benjak Horvat, Mirjana Rajilić–Stojanović

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 779 - 779

Published: March 23, 2025

Numerous factors are involved in the pathogenesis of nonalcoholic fatty liver disease (NAFLD), which responsible for its development and progression as an independent entity, but also thanks to their simultaneous action. This is explained by hypothesis multiple parallel hits. These insulin resistance, lipid metabolism alteration, oxidative stress, endoplasmic reticulum inflammatory cytokine liberation, gut microbiota dysbiosis or gut–liver axis activation. a systematic review has aim show connection between intestinal role disbalance NAFLD. The made from wide spectrum microorganisms that systemic impact on human health, with well-documented digestion, energy metabolism, stimulation immune system, synthesis essential nutrients, etc. It been shown associated all three stages chronic disease. Thus, modulation attracted research interest novel therapeutic approach management NAFLD patients. modification can be achieved substantial diet application probiotics prebiotics, while most radical effects observed fecal transplantation (FMT). Given results FMT context metabolic syndrome (MetS) animal models scarce pilot studies humans, seems promising treatment option could reverse thereby influence course

Language: Английский

Citations

0
A medium-chain fatty acid analogue prevents endotoxin liver injury in a murine model DOI Creative Commons
Sarah Wang, Thomas I. Hirsch, Scott C. Fligor

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 20, 2025

Parenteral nutrition (PN) is lifesaving for patients with short bowel syndrome and other gastrointestinal disorders, however long-term use may lead to complications including hepatosteatosis sepsis. We have previously demonstrated the anti-steatotic, -fibrotic, -inflammatory properties of SEFA-6179, an engineered medium-chain fatty acid analogue. hypothesized that SEFA-6179 treatment would protect against endotoxin-induced liver injury in a murine model PN-induced hepatosteatosis. C57Bl/6J mice were administered high-carbohydrate liquid diet plus intravenous lipid emulsion (Intralipid, 4 g fat/kg/d) or saline 19 days induce (100 mg/kg) vehicle (MCT/medium-chain triglyceride) was via oral gavage four leading up intraperitoneal challenge lipopolysaccharide (15 on day 19. Age-matched, chow-fed controls received same treatments. The primary outcome biomarkers: alanine aminotransferase aspartate aminotransferase. Pro-inflammatory cytokines, IL-6, TNF-alpha, monocyte chemoattractant protein (MCP1), analyzed. Liver immunofluorescence staining performed evaluate macrophage phenotypes. In endotoxin-challenged mice, pre-treatment lowered enzymes pro-inflammatory cytokine levels compared vehicle. On histology, led greater polarization M1/pro-inflammatory macrophages M2/anti-inflammatory phenotype currently Phase II clinical trials. These findings support potential application high-risk, PN-dependent patients.

Language: Английский

Citations

0
Investigation of parenteral nutrition-induced hepatotoxicity using human liver spheroid co-cultures DOI Creative Commons
Milos Mihajlovic, Sybren De Boever, Andrés Tabernilla

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(9), P. 3109 - 3126

Published: May 14, 2024

Abstract Parenteral nutrition (PN) is typically administered to individuals with gastrointestinal dysfunction, a contraindication for enteral feeding, and need nutritional therapy. When PN the only energy source in patients, it defined as total parenteral (TPN). TPN life-saving approach different patient populations, both infants adults. However, despite numerous benefits, can cause adverse effects, including metabolic disorders liver injury. TPN-associated injury, known intestinal failure-associated disease (IFALD), represents significant problem affecting up 90% of receiving TPN. IFALD pathogenesis complex, depending on components well patient’s medical conditions. Despite animal studies clinical observations, molecular mechanisms driving remain largely unknown. The present study was set elucidate underlying IFALD. For this purpose, human spheroid co-cultures were treated mixture, followed by RNA sequencing analysis. Subsequently, following exposure its single components, several key events mitochondrial endoplasmic reticulum stress, oxidative apoptosis, lipid accumulation (steatosis), studied using various techniques. It found that prolonged substantially changes transcriptome profile spheroids affects multiple signaling pathways contributing Moreover, main especially emulsion, induce all measured trigger steatosis.

Language: Английский

Citations

1
Adverse events associated with parenteral nutrition support therapy: A pharmacovigilance study DOI
Jing Zhou,

Xu Hongyan,

Jingjing Ma

et al.

Journal of Parenteral and Enteral Nutrition, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Background Parenteral nutrition (PN) plays a crucial role in support therapy, yet data on related adverse events (AEs) practical settings are scarce. To address this, we analyzed AE signals associated with PN treatment from the United States Food and Drug Administration Adverse Event Reporting System (FAERS) database. Methods We extracted FAERS database, covering period first quarter (Q1) of 2004 to Q1 2024. names AEs were standardized. then conducted disproportionality analyses using four different algorithms evaluate association between its AEs. Results collected total 48,890,925 reports which 1642 involved PN‐related After categorization, identified 21 system organ classes (SOCs), hepatobiliary disorders only significant SOC across all algorithms. At preferred term (PT) level, 99 PTs that showed Fat overload syndrome, fatty acid deficiency, parenteral nutrition–associated liver disease (PNALD), Malassezia infection, Pantoea agglomerans infection most prominent PTs. In addition, several potential new included nervous, cardiac, immune, psychiatric, blood, renal, urinary, eye disorders. Conclusion Our study common rare reported Patients healthcare providers should remain vigilant about these Understanding risks therapy establishing procedures can help reduce

Language: Английский

Citations

1
The use of parenteral nutrition in patients with inflammatory bowel disease: A detailed guide on the indications, risks, and benefits DOI

David Köhler,

Hannah Freid,

Jennifer Cholewka

et al.

Nutrition in Clinical Practice, Journal Year: 2024, Volume and Issue: 39(5), P. 1026 - 1036

Published: June 23, 2024

Malnutrition is estimated to affect roughly 30%-80% of patients with inflammatory bowel disease (IBD). In those who cannot tolerate sufficient oral nutrition or there no possibility for placing an enteral tube, parenteral offers a lifesaving alternative. However, this not without risk. For IBD, understanding the indications, contraindications, and complications associated crucial. review, we will discuss indications contraindications in common intravenous nutrition, use special populations, such as pediatric perioperative patients, impact on IBD-related outcomes.

Language: Английский

Citations

0
Molecular mechanisms underlying hepatoprotective activity of lutein in the context of intestinal failure-associated liver disease DOI Creative Commons

Izabela Żółnowska,

Aleksandra Gostyńska, Maciej Stawny

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 209, P. 107421 - 107421

Published: Sept. 16, 2024

Language: Английский

Citations

0
Dataset on transcriptomic profiling of parenteral nutrition-induced hepatotoxicity in a human liver in vitro model DOI Creative Commons
Milos Mihajlovic, Mathieu Vinken

Data in Brief, Journal Year: 2024, Volume and Issue: 55, P. 110653 - 110653

Published: June 18, 2024

The provided dataset describes the transcriptomic profile of human liver spheroid co-cultures consisting a hepatoma cell line (C3A/HepG2 cells) and an immortalized activated hepatic stellate (LX-2 upon exposure to total parenteral nutrition. High-throughput RNA sequencing was performed using DNBSEQTM technology. Following quality check filtering raw sequence reads, clean reads were aligned reference genome used determine differential gene expression. Raw processed data are deposited in Gene Expression Omnibus with accession number GSE264357. These could serve further mechanistic studies on nutrition-induced injury support translational research intestinal failure-associated disease occurring individuals receiving

Language: Английский

Citations

0