Young Age and Concomitant Cannabis (THC) and Ethanol (EtOH) Exposure Enhances Rat Brain Damage Through Decreased Cerebral Mitochondrial Respiration

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 918 - 918

Published: Feb. 17, 2025

The reason why young people taking concomitantly cannabis (THC) and ethanol (EtOH) are more prone to stroke is underresearched. To investigate whether an underlying mechanism of increased brain damage could be impaired mitochondrial function, this experiment determined the acute effects EtOH, both alone associated with THC, on respiration oxidative stress (hydrogen peroxide H2O2) (11 weeks) middle-aged (45 in rats, using a high-resolution oxygraph (Oxygraph-2K, Oroboros instruments). In brains, EtOH decreased by -51.76 ± 2.60% (from 32.76 3.82 17.41 1.42 pmol/s/mL, p < 0.0001). 45-week-old decrease was lesser, but still significant -36.0 2.80% 30.73 7.72 20.59 5.48 Concomitant THC aggravated decreases at 11 weeks (-86.86 1.74%, 0.0001) 45 (-73.95 3.69%, Such additional injury enhanced brains (p 0.01). H2O2 production similar age groups (1.0 0.2 versus 1.1 0.08 pmol O2/s/mL) not modified addition. conclusion, significantly impairs concomitant further aggravates such damage, particularly brains. These data support hypothesis that dysfunction might participate occurrence urge for better prevention against addictions adolescents.

Language: Английский

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Research Progress on the Protective Effect of Green Tea Polyphenol (-)-Epigallocatechin-3-Gallate (EGCG) on the Liver

Nutrients, Journal Year: 2025, Volume and Issue: 17(7), P. 1101 - 1101

Published: March 21, 2025

The liver, as the primary metabolic organ, is susceptible to an array of factors that can harm liver cells and give rise different diseases. Epigallocatechin gallate (EGCG), a natural compound found in green tea, exerts numerous beneficial effects on human body. Notably, EGCG displays antioxidative, antibacterial, antiviral, anti-inflammatory, anti-tumor properties. This review specifically highlights pivotal role liver-related diseases, focusing viral hepatitis, autoimmune fatty disease, hepatocellular carcinoma. not only inhibits entry replication hepatitis B C viruses within hepatocytes, but also mitigates hepatocytic damage caused by hepatitis-induced inflammation. Furthermore, exhibits significant therapeutic potential against Combinatorial use anti-hepatocellular carcinoma drugs enhances sensitivity drug-resistant cancer chemotherapeutic agents, leading improved outcomes. Thus, combination holds promise effective approach for treating In conclusion, possesses hepatoprotective properties various forms emerges drug candidate

Language: Английский

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Role in Preventing Alcoholic Liver Disease Progression: A Comparative Study of Whole-Component Finger Citron Essential Oil and Its Major Component D-Limonene

Nutrients, Journal Year: 2025, Volume and Issue: 17(7), P. 1255 - 1255

Published: April 3, 2025

Background/Objectives: Chronic alcohol overconsumption triggers liver injury, and therapeutic strategies targeting alcohol-triggered oxidative stress hepatic inflammatory responses represent potential approaches to ameliorating alcohol-related hepatotoxicity. This study aimed determine the hepatoprotective activity of finger citron essential oil (FCEO) in alcoholic disease (ALD)-afflicted rats explore its underlying mechanisms. In order identify effective components, we compared effects FCEO D-limonene. Methods: The regulatory on metabolic enzymes were systematically evaluated through vitro experiments. vivo studies conducted investigate compare Staining methods, assay kits, Western Blot used roles D-limonene ALD rats. Results: We found that downregulated phase I upregulated II Buffalo Rat Liver-3A (BRL-3A) cells. and/or intervention reduced transaminase levels effectively alleviated cell infiltration lipid droplet accumulation their tissue. Additionally, played a role inflammation-related pathways such as MAPK/Nrf2 NF-κB/AMPK pathways. was superior an antioxidant alleviating injury. Conclusions: revealed alleviative mechanisms demonstrating better efficacy monomer, thus providing strategy for development utilization resources.

Language: Английский

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Cannabis (THC) Aggravates the Deleterious Effects of Alcohol (EtOH) on Skeletal Muscles’ Mitochondrial Respiration: Modulation by Age and Metabolic Phenotypes

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1080 - 1080

Published: Dec. 21, 2024

The anti-inflammatory and analgesic properties of cannabis might be useful to treat muscle diseases, including those linked or not alcohol. Nevertheless, delta 9 tetrahydrocannabinol (THC) ethanol (EtOH), often used concomitantly, can have deleterious effects on cardiac mitochondria. We therefore determined whether EtOH, alone associated with THC, impairs skeletal mitochondrial respiration. Further, we investigated potential modulation by metabolic phenotype age analyzing predominantly glycolytic gastrocnemius oxidative soleus muscles in young middle-aged rats (12 49 weeks). Considering the gastrocnemius, EtOH impaired respiration a similar manner young- (-34.97 ± 2.97% vs. -37.50 6.03% at 2.1 × 10-5 M; p < 0.05). Interestingly, concomitant THC aggravated EtOH-related impairment (-49.92 1.69%, -34.97 2.97 Concerning soleus, mainly decreased (-42.39 2.42% -17.09 7.61% M, 0.001, 12 was less weeks association than ±1.69 -27.22 8.96% respectively, In conclusion, significantly aggravates EtOH-induced muscle. Age phenotypes modulate these effects, being more prone impairments muscles.

Language: Английский

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