Bacteriophage resistance increases bacterial productivity by shifting population growth dynamics in the soil bacteriumVariovoraxsp DOI Creative Commons
Meaghan Castledine, Daniel Padfield,

Rai Lewis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 7, 2024

Abstract Lytic bacteriophages (‘phages’) can limit bacterial densities, either directly through lysis or indirectly costs to resistance. However, phages have also been reported no, and in some cases even positive, effects on host densities. Here, we investigate the mechanisms behind an increase density Variovorax sp. populations following a fixation of resistance that was maintained after phage extinction. Our results demonstrate genetic trait coinciding with emergence. Growth curves showed shifted population growth such higher death-phase. This density-increasing effect had important implications for community structure resistant decreasing conspecific. That lytic densities has wider ecology therapy where are presumed negative their hosts.

Language: Английский

Emerging models to study competitive interactions within bacterial communities DOI Creative Commons
Mollie Virgo, Serge Mostowy, Brian T. Ho

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Within both abiotic and host environments, bacteria typically exist as diverse, multispecies communities have crucial roles in human health, agriculture, industry. In these communities, compete for resources, competitive interactions can shape the overall population structure community function. Studying bacterial dynamics requires experimental model systems that capture different interaction networks between their surroundings. We examine recent literature advancing such systems, including (i) silico models establishing theoretical basis how cell-to-cell influence level dynamics, (ii) vitro characterizing specific interbacterial interactions, (iii) organ-on-a-chip revealing physiologically relevant parameters, spatial mechanical forces, encounter within a host, (iv) vivo plant animal connecting responses to interactions. Each of has greatly contributed our understanding be used synergistically understand competition influences architecture.

Language: Английский

Citations

1

Influence of organophosphonates as alternative P-sources on bacterial transformation of glyphosate DOI Creative Commons

Kleanthi Kourtaki,

Daniel Büchner, Philipp R. Martin

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125872 - 125872

Published: Feb. 1, 2025

Language: Английский

Citations

0

Estimates of microbial community stability using relative invader growth rates are robust across levels of invader species richness DOI Creative Commons
Meaghan Castledine, Daniel Padfield, Angus Buckling

et al.

ISME Communications, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 1, 2025

A key feature of natural communities is that the species within them stably coexist. common metric used to test community stability ability each invade from rare. potential issue with this measurement single are invaded rare, while in communities, multiple would likely decline simultaneously following perturbations. This especially microbes which can be rapidly disturbed by environmental stressors. If coexistence dependent on indirect interactions among members, declining may result instability. As such, invading a into overestimate when decline. Here, we compare estimates five microbial experimental results invaded. Our showed invasions were qualitatively predictive whole simultaneously. However, quantitative values relative invader growth rate less comparable, being non-significantly different most comparisons three out species. was emphasized lack correlation between exact rates under or multi-species invasion. work provides support for robustness using invasion infer qualitative stability.

Language: Английский

Citations

0

Coexistence Theory for Microbial Ecology, and Vice Versa DOI Creative Commons
James Orr, David Armitage, Andrew D. Letten

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

ABSTRACT Classical models from theoretical ecology are seeing increasing uptake in microbial ecology, but there remains rich potential for closer cross‐pollination. Here we explore opportunities stronger integration of ecological theory into research (and vice versa) through the lens so‐called “modern” coexistence theory. Coexistence can be used to disentangle contributions different mechanisms (e.g., resource partitioning, environmental variability) make species coexistence. We begin with a short primer on fundamental concepts theory, an emphasis relevance communities. next present systematic review, which highlights paucity empirical applications systems. In light this gap, then identify and discuss ways which: (i) help answer applied questions particularly spatio‐temporally heterogeneous environments, (ii) experimental systems leveraged validate advance Finally, address several unique often surmountable challenges posed by systems, as well some conceptual limitations. Nevertheless, thoughtful presents wealth advancement both ecology.

Language: Английский

Citations

0

Bacteria-phage (co)evolution is constrained in a synthetic community across multiple bacteria-phage pairs DOI Creative Commons
Meaghan Castledine, Daniel Padfield, M. Corrie Schoeman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Bacteriophages can be important drivers of bacterial densities, and therefore microbial community composition function. These ecological interactions are likely to greatly affected by evolutionary dynamics, because bacteria rapidly evolve resistance phage while reciprocally increase infectivity. Most studies date have explored eco-evolutionary dynamics using isolated pairs bacteria-phage but in nature, multiple phages coexist (co)evolve simultaneously. How coevolution plays out this context is poorly understood. Here, we examine how three coexisting soil ( Ochrobactrum sp., Pseudomonas Variovorax sp.) interact with species-specific bacteriophages over eight weeks experimental evolution, both as host-parasite isolation a mixed community. Across all species evolution was inhibited polyculture, the most pronounced effect on . Between there were also substantial differences host including whether coevolved. contrasts emphasise difficulty generalising from monoculture between wider systems. Future should consider simultaneously better understand coevolutionary happen natural communities. Importance This project unique examining among their phages, rather than focus single focal – makes our work more applicable contexts still working controlled synthetic While it commonly assumed that will coevolve phage, may uncommon complex communities due reduced contact rates and/or mutation rates. Furthermore, contrast population ability highlights need for studied. Over-reliance model systems known means lack an understanding interact, what extent results generalised beyond these pairs.

Language: Английский

Citations

1

Bacteriophage resistance increases bacterial productivity by shifting population growth dynamics in the soil bacteriumVariovoraxsp DOI Creative Commons
Meaghan Castledine, Daniel Padfield,

Rai Lewis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 7, 2024

Abstract Lytic bacteriophages (‘phages’) can limit bacterial densities, either directly through lysis or indirectly costs to resistance. However, phages have also been reported no, and in some cases even positive, effects on host densities. Here, we investigate the mechanisms behind an increase density Variovorax sp. populations following a fixation of resistance that was maintained after phage extinction. Our results demonstrate genetic trait coinciding with emergence. Growth curves showed shifted population growth such higher death-phase. This density-increasing effect had important implications for community structure resistant decreasing conspecific. That lytic densities has wider ecology therapy where are presumed negative their hosts.

Language: Английский

Citations

0