Rat superior colliculus encodes the transition between static and dynamic vision modes DOI Creative Commons
Rita Gil, Mafalda Valente, Noam Shemesh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 27, 2022

Abstract When visual stimuli are presented at a sufficiently high temporal frequency, perception shifts from the static to dynamic vision mode, thereby facilitating continuity illusion which is key for correctly identifying continuous and moving objects placing them in context of surrounding environment. However, how this encoded along entire pathway remains poorly understood, with disparate Flicker Fusion Frequency (FFF) thresholds measured retinal, cortical, behavioural levels. Here, we hypothesized that these disparities may suggest other brain areas be involved encoding shift modes. We employ comprehensive approach encompassing measurements, whole activation mapping fidelity functional MRI (fMRI), local electrophysiological validation studying mechanisms underlying modes rat. Our measurements reported an FFF threshold proxy 18±2 Hz. At network level, revealed superior colliculus (SC) exhibits marked signal transitions positive negative fMRI regimes behaviourally surrogates, strong linear correlation between behaviour, while thalamic cortical displayed significantly poorer behaviour. fMRI-driven neurometric curves approximated psychometric curve SC but not areas. Electrophysiological recordings suggested signals arise neural activation/suppression low/high frequency regimes, respectively, transition occurs around proxies. Lesions V1 further reinforced originate SC. Combined, our data suggests critical role discriminations, particular

Language: Английский

Distinct neurochemical influences on fMRI response polarity in the striatum DOI Creative Commons
Domenic H. Cerri, Daniel L. Albaugh, Lindsay R. Walton

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 1, 2024

Abstract The striatum, known as the input nucleus of basal ganglia, is extensively studied for its diverse behavioral roles. However, relationship between neuronal and vascular activity, vital interpreting functional magnetic resonance imaging (fMRI) signals, has not received comprehensive examination within striatum. Here, we demonstrate that optogenetic stimulation dorsal striatal neurons or their afferents from various cortical subcortical regions induces negative fMRI responses in rats, manifesting vasoconstriction. These occur even with heightened confirmed by electrophysiology fiber-photometry. In parallel, midbrain dopaminergic neuron modulation, coupled electrochemical measurements, establishes a link vasodilation dopamine release. Intriguingly, vivo intra-striatal pharmacological manipulations during highlight critical role opioidergic signaling generating This observation substantiated detecting vasoconstriction brain slices after synthetic opioid application. humans, aimed at increasing activity likewise elicit responses. Our results emphasize necessity considering vasoactive neurotransmission alongside when signal.

Language: Английский

Citations

17
Rat superior colliculus encodes the transition between static and dynamic vision modes DOI Creative Commons
Rita Gil, Mafalda Valente, Noam Shemesh

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 12, 2024

The visual continuity illusion involves a shift in perception from static to dynamic vision modes when the stimuli arrive at high temporal frequency, and is critical for recognizing objects moving environment. However, how this encoded across pathway remains poorly understood, with disparate frequency thresholds retinal, cortical, behavioural levels suggesting involvement of other brain areas. Here, we employ multimodal approach encompassing behaviour, whole-brain functional MRI, electrophysiological measurements, investigating encoding rats. Behavioural experiments report threshold 18±2 Hz. Functional MRI reveal that superior colliculus signals transition positive negative behaviourally-driven threshold, unlike thalamic cortical Electrophysiological recordings indicate these transitions are underpinned by neural activation/suppression. Lesions primary cortex effect be intrinsic (under gain effect). Our findings highlight colliculus' crucial shifts, especially change modes.

Language: Английский

Citations

7
Acute alcohol induces greater dose-dependent increase in the lateral cortical network functional connectivity in adult than adolescent rats DOI Creative Commons
Sung‐Ho Lee, Tatiana A. Shnitko, Li‐Ming Hsu

et al.

Addiction Neuroscience, Journal Year: 2023, Volume and Issue: 7, P. 100105 - 100105

Published: June 2, 2023

Alcohol misuse and, particularly adolescent drinking, is a major public health concern. While evidence suggests that alcohol use affects frontal brain regions are important for cognitive control over behavior little known about how acute exposure alters large-scale networks and sex age may moderate such effects. Here, we employ recently developed functional magnetic resonance imaging (fMRI) protocol to acquire rat connectivity data an established analytical pipeline examine the effect of sex, age, dose on within between three rodent networks: defaul mode, salience, lateral cortical network. We identify intra- inter-network differences establish moderation models reveal significant influences alcohol-induced network connectivity. Through this work, make brain-wide isotropic fMRI with challenge publicly available, hope facilitate future discovery regions/circuits causally relevant impact use.

Language: Английский

Citations

7
Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation DOI Creative Commons
Yuhui Li, Sung-Ho Lee, Chunxiu Yu

et al.

Brain stimulation, Journal Year: 2024, Volume and Issue: 17(4), P. 947 - 957

Published: July 1, 2024

Language: Английский

Citations

1
Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation DOI Open Access
Yuhui Li, Sung‐Ho Lee, Chunxiu Yu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 27, 2024

Summary While deep brain stimulation (DBS) is widely employed for managing motor symptoms in Parkinson’s disease (PD), its exact circuit mechanisms remain controversial. To identify the neural targets affected by therapeutic DBS PD, we analyzed DBS-evoked whole activity female hemi-parkinsonian rats using function magnetic resonance imaging (fMRI). We delivered subthalamic nucleus (STN) at various pulse repetition rates optogenetics, allowing unbiased examinations of cell-type specific STN feed-forward activity. Unilateral optogenetic elicited rate-dependent alterations blood-oxygenation-level-dependent (BOLD) signals SNr (substantia nigra pars reticulata), GP (globus pallidus), and CPu (caudate putamen). Notably, these manipulations effectively ameliorated pathological circling behavior animals expressing kinetically faster Chronos opsin, but not ChR2. Furthermore, mediation analysis revealed that behavioral rescue was significantly mediated optogenetically induced changes CPu, SNr. This suggests activation are critically involved DBS.

Language: Английский

Citations

0
Active Suppression of the Nigrostriatal Pathway during Optogenetic Stimulation Revealed by Simultaneous fPET/fMRI DOI Creative Commons
Sabrina Haas, Fernando Bravo,

Tudor M. Ionescu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 20, 2023

Abstract The dopaminergic system is a central component of the brain’s neurobiological framework, governing motor control, reward responses, and playing an essential role in various brain disorders such as Parkinson’s disease schizophrenia. Within this complex network, nigrostriatal pathway represents critical circuit for dopamine transmission from substantia nigra to striatum, connection that vital understanding many disease-related dysfunctions. However, stand-alone functional magnetic resonance imaging (fMRI) unable study intricate interplay between activation its molecular underpinnings. In our study, simultaneous use [ 18 F]FDG positron emission tomography (fPET)/BOLD-fMRI provided new insight allowed us demonstrate active suppression activity during optogenetic stimulation via presynaptic autoinhibition. Our vivo observation emphasizes observed BOLD signal depression neuronal does not correlate with inactivity, but results firing shown by high increase. This result only illustrates potential fPET/fMRI understand mechanisms function also provides perspective on how neurotransmitters influence hemodynamic responses brain.

Language: Английский

Citations

1
Rat superior colliculus encodes the transition between static and dynamic vision modes DOI Creative Commons
Rita Gil, Mafalda Valente, Noam Shemesh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 27, 2022

Abstract When visual stimuli are presented at a sufficiently high temporal frequency, perception shifts from the static to dynamic vision mode, thereby facilitating continuity illusion which is key for correctly identifying continuous and moving objects placing them in context of surrounding environment. However, how this encoded along entire pathway remains poorly understood, with disparate Flicker Fusion Frequency (FFF) thresholds measured retinal, cortical, behavioural levels. Here, we hypothesized that these disparities may suggest other brain areas be involved encoding shift modes. We employ comprehensive approach encompassing measurements, whole activation mapping fidelity functional MRI (fMRI), local electrophysiological validation studying mechanisms underlying modes rat. Our measurements reported an FFF threshold proxy 18±2 Hz. At network level, revealed superior colliculus (SC) exhibits marked signal transitions positive negative fMRI regimes behaviourally surrogates, strong linear correlation between behaviour, while thalamic cortical displayed significantly poorer behaviour. fMRI-driven neurometric curves approximated psychometric curve SC but not areas. Electrophysiological recordings suggested signals arise neural activation/suppression low/high frequency regimes, respectively, transition occurs around proxies. Lesions V1 further reinforced originate SC. Combined, our data suggests critical role discriminations, particular

Language: Английский

Citations

1