A host of armor: Prokaryotic immune strategies against mobile genetic elements
Cell Reports,
Journal Year:
2023,
Volume and Issue:
42(7), P. 112672 - 112672
Published: June 21, 2023
Prokaryotic
adaptation
is
strongly
influenced
by
the
horizontal
acquisition
of
beneficial
traits
via
mobile
genetic
elements
(MGEs),
such
as
viruses/bacteriophages
and
plasmids.
However,
MGEs
can
also
impose
a
fitness
cost
due
to
their
often
parasitic
nature
differing
evolutionary
trajectories.
In
response,
prokaryotes
have
evolved
diverse
immune
mechanisms
against
MGEs.
Recently,
our
understanding
abundance
diversity
prokaryotic
systems
has
greatly
expanded.
These
defense
degrade
invading
material,
inhibit
genome
replication,
or
trigger
abortive
infection,
leading
population
protection.
this
review,
we
highlight
these
strategies,
focusing
on
most
recent
discoveries.
The
study
defenses
not
only
sheds
light
microbial
evolution
but
uncovers
novel
enzymatic
activities
with
promising
biotechnological
applications.
Language: Английский
Correlation of Pseudomonas aeruginosa Phage Resistance with the Numbers and Types of Antiphage Systems
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(3), P. 1424 - 1424
Published: Jan. 24, 2024
Phage
therapeutics
offer
a
potentially
powerful
approach
for
combating
multidrug-resistant
bacterial
infections.
However,
to
be
effective,
phage
therapy
must
overcome
existing
and
developing
resistance.
While
cocktails
can
reduce
this
risk
by
targeting
multiple
receptors
in
single
therapeutic,
bacteria
have
mechanisms
of
resistance
beyond
receptor
modification.
A
rapidly
growing
body
knowledge
describes
broad
varied
arsenal
antiphage
systems
encoded
counter
infection.
We
sought
understand
the
types
frequencies
present
highly
diverse
panel
Pseudomonas
aeruginosa
clinical
isolates
utilized
characterize
novel
antibacterials.
Using
web-server
tool
PADLOC
(prokaryotic
antiviral
defense
locator),
putative
were
identified
these
P.
based
on
sequence
homology
validated
curated
catalog
known
systems.
Coupling
host
bacterium
analysis
with
range
data
70
phages,
we
observed
correlation
between
presence
higher
numbers
genomes.
also
able
identify
that
more
prevalent
phage-resistant
strains,
suggesting
their
importance
conferring
Language: Английский
Modularity of Zorya defense systems during phage inhibition
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 8, 2025
Abstract
Bacteria
have
evolved
an
extraordinary
diversity
of
defense
systems
against
bacteriophage
(phage)
predation.
However,
the
molecular
mechanisms
underlying
these
anti-phage
often
remain
elusive.
Here,
we
provide
mechanistic
and
structural
insights
into
Zorya
phage
systems.
Using
cryo-EM
analyses,
show
that
type
I
II
core
components,
ZorA
ZorB,
assemble
in
a
5:2
complex
is
similar
to
inner-membrane
ion-driven,
rotary
motors
power
flagellar
rotation,
9
secretion,
gliding
Ton
nutrient
uptake
The
ZorAB
has
elongated
cytoplasmic
tail
assembled
by
bundling
C-termini
five
subunits.
Mutagenesis
demonstrates
peptidoglycan
binding
periplasmic
domains
structured
ZorA,
ion
flow
through
motor
important
for
function
both
Furthermore,
identify
ZorE
as
effector
module
system,
possessing
nickase
activity.
Our
work
reveals
basis
activity
highlights
crucial
population-wide
immunity
system.
Language: Английский
Correlation of Pseudomonas aeruginosa Phage Resistance with Numbers and Types of Antiphage Systems
Published: Dec. 18, 2023
Phage
therapeutics
offer
a
potentially
powerful
approach
to
combat
multidrug-resistant
bacterial
infections.
However,
be
effective,
phage
therapy
must
overcome
existing
and
developing
resistance.
While
cocktails
can
reduce
this
risk
by
targeting
multiple
receptors
in
single
therapeutic,
bacteria
have
mechanisms
of
resistance
beyond
receptor
modification.
A
rapidly
growing
body
knowledge
describes
broad
varied
arsenal
antiphage
systems
encoded
counter
infection.
We
sought
understand
the
types
frequencies
present
highly
diverse
panel
Pseudomonas
aeruginosa
clinical
isolates
utilized
characterize
novel
antibacterials.
Using
web-server
tool
PADLOC
(Prokaryotic
Antiviral
Defense
Locator),
putative
were
identified
these
P.
based
on
sequence
homology
validated
curated
catalog
known
defense
systems.
Coupling
host
bacterium
analysis
with
range
data
for
70
phages,
we
observed
correlation
between
presence
higher
numbers
genomes.
also
able
identify
that
more
prevalent
phage-resistant
strains,
suggesting
their
importance
conferring
Language: Английский