NADase CD38 is a key determinant of ovarian aging
Nature Aging,
Journal Year:
2023,
Volume and Issue:
4(1), P. 110 - 128
Published: Dec. 21, 2023
Abstract
The
ovary
ages
earlier
than
most
other
tissues,
yet
the
underlying
mechanisms
remain
elusive.
Here
a
comprehensive
analysis
of
transcriptomic
landscapes
in
different
organs
young
and
middle-aged
mice
revealed
that
ovaries
showed
expression
age-associated
genes,
identifying
increased
NADase
CD38
decreased
NAD
+
levels
mice.
Bulk
single-cell
RNA
sequencing
deletion
mitigated
ovarian
aging,
preserving
fertility
follicle
reserve
aged
by
countering
age-related
gene
changes
intercellular
communication
alterations.
Mechanistically,
onset
inflammation
induced
higher
ovary,
thereby
accelerating
aging.
Consistently,
pharmacological
inhibition
enhanced
Our
findings
aging
relative
to
organs,
providing
potential
therapeutic
target
for
ameliorating
female
infertility.
Language: Английский
Aging hallmarks of the primate ovary revealed by spatiotemporal transcriptomics
Huifen Lu,
No information about this author
Ying Jing,
No information about this author
Chen Zhang
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et al.
Protein & Cell,
Journal Year:
2023,
Volume and Issue:
15(5), P. 364 - 384
Published: Dec. 21, 2023
The
ovary
is
indispensable
for
female
reproduction,
and
its
age-dependent
functional
decline
the
primary
cause
of
infertility.
However,
molecular
basis
ovarian
aging
in
higher
vertebrates
remains
poorly
understood.
Herein,
we
apply
spatiotemporal
transcriptomics
to
benchmark
architecture
organization
as
well
cellular
determinants
young
primate
ovaries
compare
these
aged
ovaries.
From
a
global
view,
somatic
cells
within
non-follicle
region
undergo
more
pronounced
transcriptional
fluctuation
relative
those
follicle
region,
likely
constituting
hostile
microenvironment
that
facilitates
aging.
Further,
uncovered
inflammation,
senescent-associated
secretory
phenotype,
senescence,
fibrosis
are
contributors
(PCOA).
Of
note,
identified
spatial
co-localization
between
PCOA-featured
spot
an
unappreciated
MT2
(Metallothionein
2)
highly
expressing
(MT2high)
characterized
by
high
levels
potentially
serving
hotspot
ovary.
Moreover,
with
advanced
age,
subpopulation
MT2high
accumulates,
disseminating
amplifying
senescent
signal
outward.
Our
study
establishes
first
transcriptomic
atlas,
advancing
our
understanding
mechanistic
underpinning
unraveling
potential
biomarkers
therapeutic
targets
age-associated
human
disorders.
Language: Английский
Analysis of cell–cell interaction between mural granulosa cells and cumulus granulosa cells during ovulation using single‐cell RNA sequencing data of mouse ovary
Reproductive Medicine and Biology,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: Jan. 1, 2024
We
investigated
the
interactions
between
mural
granulosa
cells
(MGCs)
and
cumulus
(CGCs)
during
ovulation
after
LH
surge.
Language: Английский
Transcriptomic Analysis Reveals That Excessive Thyroid Hormone Signaling Impairs Phototransduction and Mitochondrial Bioenergetics and Induces Cellular Stress in Mouse Cone Photoreceptors
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(13), P. 7435 - 7435
Published: July 6, 2024
Thyroid
hormone
(TH)
plays
an
essential
role
in
cell
proliferation,
differentiation,
and
metabolism.
Experimental
clinical
studies
have
shown
a
potential
association
between
TH
signaling
retinal
degeneration.
The
suppression
of
protects
cone
photoreceptors
mouse
models
degeneration,
whereas
excessive
induces
manifested
as
reduced
light
response
loss
cones.
This
work
investigates
the
genes/transcriptomic
alterations
that
might
be
involved
TH-induced
degeneration
mice
using
single-cell
RNA
sequencing
(scRNAseq)
analysis.
One-month-old
C57BL/6
received
triiodothyronine
(T3,
20
µg/mL
drinking
water)
for
4
weeks
model
hyperthyroidism/excessive
signaling.
At
end
experiments,
cells
were
dissociated,
viability
was
analyzed
before
being
subjected
to
scRNAseq.
resulting
data
Seurat
package
visualized
Loupe
browser.
Among
155,866
single
cells,
we
identified
14
clusters,
representing
various
types,
with
rod
clusters
comprising
76%
4.1%
total
population,
respectively.
Cone
cluster
transcriptomes
demonstrated
most
after
T3
treatment,
450
differentially
expressed
genes
(DEGs),
accounting
38.5%
DEGs.
Statistically
significant
changes
expression
revealed
phototransduction
oxidative
phosphorylation
impaired
along
mitochondrial
dysfunction.
A
pathway
analysis
also
showed
activation
sensory
neuronal/photoreceptor
stress
pathways
treatment.
Specifically,
eukaryotic
initiation
factor-2
cAMP
element-binding
protein
upregulated.
Thus,
substantially
affects
cones
at
transcriptomic
level.
findings
from
this
provide
insight
into
how
Language: Английский