Bacterial multispecies invasion of human epithelial bladder cells DOI Creative Commons

Charlotte Abell-King,

Alaska Pokhrel, Stuart A. Rice

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 17, 2023

Abstract Urinary tract infections (UTI) are one of the most common bacterial worldwide. While overall infection course is known on a macroscale, behavior not fully understood at cellular level and pathophysiology during multispecies well characterized. Here we establish co-infection models combined with high resolution imaging to compare single- multi-species bladder cell invasion events in three uropathogens: uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae Enterococcus faecalis . all species invaded cells, under flow conditions Gram-positive E. was significantly less invasive compared Gram-negative UPEC K. When introduced simultaneously an experiment, sometimes same cell, differing frequencies suggesting complex interactions between cells. Inside host observed encasement colonies specifically by UPEC. During subsequent dispersal from only bacteria underwent infection-related filamentation (IRF). Taken together, our data suggest that invasions single cells frequent support earlier studies showing intraspecies cooperation biochemical UTI.

Language: Английский

Multispecies bacterial invasion of human host cells DOI Creative Commons

Charlotte Abell-King,

Alaska Pokhrel, Stuart A. Rice

et al.

Pathogens and Disease, Journal Year: 2024, Volume and Issue: 82

Published: Jan. 1, 2024

Abstract Urinary tract infection (UTI), one of the most common bacterial infections worldwide, is a typical example an that often polymicrobial in nature. While overall course known on macroscale, behavior not fully understood at cellular level and pathophysiology during multispecies well characterized. Here, using clinically relevant bacteria, human epithelial bladder cells urine, we establish co-infection models combined with high resolution imaging to compare single- multi-species cell invasion events three uropathogens: uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae Enterococcus faecalis. all species invaded cells, under flow conditions Gram-positive E. faecalis was significantly less invasive compared Gram-negative UPEC K. pneumoniae. When introduced simultaneously experiment, sometimes same cell, differing frequencies suggesting complex interactions between cells. Inside host observed encasement colonies specifically by UPEC. During subsequent dispersal from only bacteria underwent infection-related filamentation (IRF). Taken together, our data suggest invasions single are frequent support earlier studies showing intraspecies cooperation biochemical UTI.

Language: Английский

Citations

3
Bacterial multispecies invasion of human epithelial bladder cells DOI Creative Commons

Charlotte Abell-King,

Alaska Pokhrel, Stuart A. Rice

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 17, 2023

Abstract Urinary tract infections (UTI) are one of the most common bacterial worldwide. While overall infection course is known on a macroscale, behavior not fully understood at cellular level and pathophysiology during multispecies well characterized. Here we establish co-infection models combined with high resolution imaging to compare single- multi-species bladder cell invasion events in three uropathogens: uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae Enterococcus faecalis . all species invaded cells, under flow conditions Gram-positive E. was significantly less invasive compared Gram-negative UPEC K. When introduced simultaneously an experiment, sometimes same cell, differing frequencies suggesting complex interactions between cells. Inside host observed encasement colonies specifically by UPEC. During subsequent dispersal from only bacteria underwent infection-related filamentation (IRF). Taken together, our data suggest that invasions single cells frequent support earlier studies showing intraspecies cooperation biochemical UTI.

Language: Английский

Citations

0