N-Glycome Profile of the Spike Protein S1: Systemic and Comparative Analysis from Eleven Variants of SARS-CoV-2 DOI Creative Commons
Cristian D. Gutierrez Reyes, Sherifdeen Onigbinde, Akeem Sanni

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(9), P. 1421 - 1421

Published: Sept. 20, 2023

The SARS-CoV-2 virus rapidly spread worldwide, threatening public health. Since it emerged, the scientific community has been engaged in development of effective therapeutics and vaccines. subunit S1 spike protein mediates viral entry into host is therefore one major research targets. extensively glycosylated, there compelling evidence that glycans protect virus’ active site from human defense system. Therefore, investigation glycome alterations different variants will provide a view glycan evolution its relationship with pathogenesis. In this study, we explored N-glycosylation expression for eleven variants: five concern (VOC), including alpha, beta, gamma, delta, omicron, six interest (VOI), epsilon, eta, iota, lambda, kappa, mu. results showed significant differences N-glycome abundance all variants. VOC large increase sialofucosylated glycans, greatest omicron variant. contrast, fucosylated most VOI. Two compositions, GlcNAc4,Hex5,Fuc,NeuAc (4-5-1-1) GlcNAc6,Hex8,Fuc,NeuAc (6-8-1-1), were abundant structures across We believe our data contribute to understanding protein’s structural between mutations.

Language: Английский

Architects of infection: A structural overview of SARS-related coronavirus spike glycoproteins DOI Creative Commons

Francesca R. Hills,

Jemma L. Geoghegan, Mihnea Bostina

et al.

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110383 - 110383

Published: Jan. 1, 2025

The frequency of zoonotic viral emergence within the Coronaviridae family highlights critical need to understand structural features spike proteins that govern entry and host adaptation. Investigating conservation variation in key regions protein-those involved range, binding affinity, entry, immune evasion-is essential for predicting evolutionary pathways coronaviruses, assessing risk future host-jumping events, discovering pan-neutralising antibodies. Here we summarise our current understanding similar SARS-CoV-2 from compare functional similarities differences. Our aim is demonstrate significant sequence between a range species outline importance animal coronavirus surveillance investigation endeavour pandemic preparedness against emerging viruses.

Language: Английский

Citations

1
Cleaved vs. Uncleaved: How Furin Cleavage Reshapes the Conformational Landscape of SARS-CoV-2 Spike DOI Creative Commons

Natesan Mani,

Raghavendran Suresh,

Srirupa Chakraborty

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

The SARS-CoV-2 Spike protein is the primary target for vaccine design, with immunogens typically engineered to enhance stability by introducing proline mutations (2P) and mutating or deleting Furin Cleavage Site (FCS). While these modifications improve structural integrity, studies suggest that furin cleavage can play a functional role in dynamics, potentially enhancing ACE2 receptor binding. However, impact of this on unbound form remains unclear. In study, we use extensive all-atom molecular dynamics (MD) simulations compare dynamic properties cleaved uncleaved proteins their pre-fusion, state. Our results show significantly alters allosteric communication within protein, increasing correlated motions between Receptor Binding Domain (RBD) N-terminal (NTD), which may facilitate engagement. Principal Component Analysis (PCA) reveals sample distinct conformational landscapes, displaying enhanced flexibility broader range RBD tilt angles. Additionally, primes S2 subunit expanding central helix, influencing transition post-fusion Glycan clustering patterns further an adaptive response cleavage, particularly NTD regions. These findings highlight potential consequences FCS deletion immunogen design underscore importance considering native state therapeutic development.

Language: Английский

Citations

0
N-Glycome Profile of the Spike Protein S1: Systemic and Comparative Analysis from Eleven Variants of SARS-CoV-2 DOI Creative Commons
Cristian D. Gutierrez Reyes, Sherifdeen Onigbinde, Akeem Sanni

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(9), P. 1421 - 1421

Published: Sept. 20, 2023

The SARS-CoV-2 virus rapidly spread worldwide, threatening public health. Since it emerged, the scientific community has been engaged in development of effective therapeutics and vaccines. subunit S1 spike protein mediates viral entry into host is therefore one major research targets. extensively glycosylated, there compelling evidence that glycans protect virus’ active site from human defense system. Therefore, investigation glycome alterations different variants will provide a view glycan evolution its relationship with pathogenesis. In this study, we explored N-glycosylation expression for eleven variants: five concern (VOC), including alpha, beta, gamma, delta, omicron, six interest (VOI), epsilon, eta, iota, lambda, kappa, mu. results showed significant differences N-glycome abundance all variants. VOC large increase sialofucosylated glycans, greatest omicron variant. contrast, fucosylated most VOI. Two compositions, GlcNAc4,Hex5,Fuc,NeuAc (4-5-1-1) GlcNAc6,Hex8,Fuc,NeuAc (6-8-1-1), were abundant structures across We believe our data contribute to understanding protein’s structural between mutations.

Language: Английский

Citations

8