The SLC19A1-AS/miR-1343/WNT11 axis is a novel positive regulatory ceRNA network governing goat granulosa cell proliferation

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130658 - 130658

Published: March 12, 2024

Language: Английский

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In situ characterization of the tumor microenvironment

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 86, P. 103083 - 103083

Published: Feb. 20, 2024

The development of new therapies for cancer is underpinned by an increasing need to comprehensively characterize the tumor microenvironment (TME). While traditional approaches have relied on bulk or single-cell approaches, these are limited in their ability provide cellular context. Deconvolution complex TME fundamental understanding dynamics and treatment resistance. Spatially resolved characterization likely greater insights into architecture, tumor-immune cell interactions, receptor-ligand niches. In turn, aid dictating optimal way which target each patient's individual cancer. this review, we discuss a number cutting-edge situ spatial profiling methods giving us biology.

Language: Английский

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Comparison of Steroidogenic and Ovulation-Inducing Effects of Orthosteric and Allosteric Agonists of Luteinizing Hormone/Chorionic Gonadotropin Receptor in Immature Female Rats

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16618 - 16618

Published: Nov. 22, 2023

Gonadotropins, including human chorionic gonadotropin (hCG), are used to induce ovulation, but they have a number of side effects, ovarian hyperstimulation syndrome (OHSS). A possible alternative is allosteric luteinizing hormone (LH)/hCG receptor agonists, the compound TP4/2 we developed, which remains active when administered orally. The aim was study effectiveness (orally, 40 mg/kg) as an ovulation inducer in FSH-stimulated immature female rats, compared with hCG (s.c., 15 IU/rat). stimulated progesterone production and corpus luteum formation; time-dependently increased expression steroidogenic genes (Star, Cyp11a1, Cyp17a1) involved regulation (Adamts-1, Cox-2, Egr-1, Mt-1); content metalloproteinase ADAMTS-1 ovaries. These effects were similar those hCG, although some cases less pronounced. TP4/2, contrast maintained normal LH levels LH/hCG gene, indicating preservation sensitivity LH, did not cause sustained increase vascular endothelial growth factor-A OHSS. Thus, effective that, unlike has lower risk OHSS resistance due its moderate stimulating effect on steroidogenesis.

Language: Английский

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Rapid and memory-efficient analysis and quality control of large spatial transcriptomics datasets

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract The 10x Visium spatial transcriptomics platform has been widely adopted due to its established analysis pipelines, robust community support, and manageable data output. However, technologies like have the limitation of being low-resolution, recently platforms with subcellular resolution proliferated. Such high-resolution datasets pose significant computational challenges for analysis, regards memory requirement processing speed. Here, we introduce Pseudovisium, a Python-based framework designed facilitate rapid memory-efficient quality control interoperability data. This is achieved by mimicking structure through hexagonal binning transcripts. Analysis 47 publicly available concluded that Pseudovisium increased speed reduced dataset size more than an order magnitude. At same time, it preserved key biological signatures, such as spatially variable genes, enriched gene sets, cell populations, gene-gene correlations. allows accurate simulation experiments, facilitating comparisons between guiding experimental design. Specifically, found high concordance (derived from Xenium or CosMx) consecutive tissue slices. We further demonstrate Pseudovisium’s utility performing on large-scale Xenium, CosMx, MERSCOPE platforms, identifying similar replicates, well potentially low-quality samples probes. common format provided also enabled direct comparison metrics across 6 59 datasets, revealing differences in transcript capture efficiency quality. Lastly, merging joint demonstrated identification shared clusters sets mouse brain using multiple platforms. By lowering requirements enhancing reusability data, democratizes wet-lab scientists enables novel insights.

Language: Английский

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Dynamic changes in cellular atlases and communication patterns within yak ovaries across diverse reproductive states unveiled by single-cell analysis

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 29, 2024

Yaks ( Bos grunniens ) exhibit exceptional adaptation to the challenging high-altitude environment of Qinghai-Tibetan plateau, making them sole bovine species capable thriving in such exreme conditions. Investigating cellular and molecular characteristics yak ovaries across different reproductive states is crucial for gaining insight into their ovarian functions. Herein, atlases were depicted by single-cell RNA-sequencing (scRNA-seq). The established identifying specific gene expression patterns various cell types, including granulosa cells, theca stromal smooth muscle endothelial glial cell, macrophages, natural killer proliferating cells. compositions vary among states. Furthermore, cells comprise six subtypes, while consist eight subtypes. distinct biological functions throughout two types aligned along respective pseudotime trajectories. Moreover, a cell-to-cell communication network was constructed within ovary, spanning three Notably, during estrus period, demonstrated more prominent interactions with other compared remaining

Language: Английский

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scRNA-seq revealing the molecular atlas of the goat follicular microenvironment over the time course of ovulation

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

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