bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 15, 2024
Our sense of taste is critical for regulating food consumption. The fruit fly
Language: Английский
Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11
Published: April 11, 2024
Across species, taste provides important chemical information about potential food sources and the surrounding environment. As details chemicals receptors responsible for gustation are discovered, a complex view of system is emerging with significant contributions from research using fruit fly, Drosophila melanogaster , as model organism. In this brief review, we summarize recent advances in their relevance to more broadly. Our goal highlight molecular mechanisms underlying first step gustatory circuits: ligand-receptor interactions primary cells. After an introduction how it encodes canonical modalities sweet, bitter, salty, describe insights into nature carboxylic acid amino detection context sour umami taste, respectively. analysis extends non-canonical including metals, fatty acids, bacterial components, highlights unexpected signaling pathways that have recently been identified Comparing intricate cellular underpinnings ligands detected vivo flies reveals both specific promiscuous receptor selectivity encoding. Throughout compare contextualize these findings mammalian not only emphasize conservation chemosensory systems, but demonstrate power organism elucidating neurobiology feeding.
Language: Английский
Citations
5
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 23, 2024
ABSTRACT Chemosensory cells across the body of Drosophila melanogaster evaluate environment and play a crucial role in neural circuits that prioritize feeding, mating, or egg laying. Previous mapping gustatory receptor neurons (GRNs) on fly labellum identified set L-type sensilla defined by expression Ionotropic Receptor 94e (IR94e), but impact IR94e GRNs behavior remained unclear. To understand their behavioral output, we used optogenetics chemogenetics to activate found they drive mild suppression feeding enhanced In vivo calcium imaging revealed respond strongly certain amino acids, including glutamate. Furthermore, is necessary sufficient for detection acid ligands, co-receptors IR25a IR76b are also required GRN activation. Finally, mutants show changes solutions containing increased consumption decreased Overall, our results suggest discourage encourage laying as part an important switch response chemical cues.
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 26, 2024
Abstract Taste is crucial for many innate and learned behaviors. In the fly, taste impacts feeding, oviposition, locomotion, mating, memory formation, to name a few. These diverse roles may necessitate apparent distributed nature of responses across different circuits in fly brain, leading complexity that has hindered attempts deduce unifying principles processing coding. Here, we combine information from whole brain connectome with functional calcium imaging examine neural representation at early steps processing. We find quality remains largely segregated cholinergic GABAergic local interneurons (LNs) are directly postsynaptic sensory neurons labellum. Although some projection (TPNs) projecting superior protocerebrum receive direct inputs neurons, primarily indirect via LNs. Moreover, found appear function as nodes convey feedforward dedicated sets morphologically similar TPNs. Examining small number representative TPNs suggests mostly this level well. Together, these studies suggest previously unappreciated logic organization circuits.
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 15, 2024
Our sense of taste is critical for regulating food consumption. The fruit fly
Language: Английский
Citations
1