Identifying the interactome of altered ion channels with lipid metabolism in SARS-CoV-2 infected patients in post-COVID-19 era DOI Creative Commons

John Philip George,

Kaushik Parthasarathi,

Kiran Bharat Gaikwad

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Abstract The Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection continues to expand its horizon through the development of diverse symptoms, particularly concerning long COVID. patients infected with SARS-CoV-2 are being reported develop new symptoms such as brain fog, fatigue, and other that not limited system. utilizes human ion channels (HICs) molecules involved in lipid metabolism from their entry egress. Here, identify molecular alterations HICs metabolism-related genes, transcriptomic data 277 were analyzed. 287 754 genes found be differentially expressed patients. Further, an interactome altered proteins was generated. Extensive mining approach employed generate a pathway map highlighting alteration several pathways including calcium signaling, long-term depression, cholesterol Moreover, 17 potential drugs known modes action interact 4 inositol 1,4,5-triphosphate (InsP3) receptors gap junction protein alpha 1 identified. Most likely, these candidates for drug repurposing require further experimental validation.

Language: Английский

An integrated bioinformatics approach reveals the potential role of microRNA-30b-5p and let-7a-5p during SARS CoV-2 spike-1 mediated neuroinflammation DOI Creative Commons
Puja Pawar,

Kadambari Akolkar,

Vandana Saxena

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134329 - 134329

Published: Aug. 7, 2024

SARS-CoV-2 induced neuroinflammation contributing to neurological sequelae is one of the critical outcomes long-COVID, however underlying regulatory mechanisms involved therein are poorly understood. We deciphered profile dysregulated microRNAs, their targets, associated pathways, protein-protein interactions (PPI), transcription factor-hub genes interaction networks, hub genes-microRNA co-regulatory networks in Spike-1 (S1) stimulated microglial cells along with candidate drug prediction using RNA-sequencing and multiple bioinformatics approaches. identified 11 microRNAs S1-stimulated (p < 0.05). KEGG analysis revealed involvement important neuroinflammatory pathways such as MAPK signalling, PI3K-AKT Ras signalling axon guidance. PPI further these pathways. Real time PCR validation confirmed a significant upregulation microRNA-30b-5p let-7a-5p; proinflammatory cytokines- IL-6, TNF-α, IL-1β, GM-CSF; inflammatory genes- PIK3CA AKT cells, while PTEN SHIP1 expression was decreased compared non-stimulated cells. Drug indicated resveratrol, diclofenac rapamycin potential drugs based on degree genes. Thus, targeting and/or intermediate molecules would be prospective immunotherapeutic approach alleviating SARS-CoV-2-S1 mediated neuroinflammation; needs investigations.

Language: Английский

Citations

1
Identifying the interactome of altered ion channels with lipid metabolism in SARS-CoV-2 infected patients in post-COVID-19 era DOI Creative Commons

John Philip George,

Kaushik Parthasarathi,

Kiran Bharat Gaikwad

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Abstract The Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection continues to expand its horizon through the development of diverse symptoms, particularly concerning long COVID. patients infected with SARS-CoV-2 are being reported develop new symptoms such as brain fog, fatigue, and other that not limited system. utilizes human ion channels (HICs) molecules involved in lipid metabolism from their entry egress. Here, identify molecular alterations HICs metabolism-related genes, transcriptomic data 277 were analyzed. 287 754 genes found be differentially expressed patients. Further, an interactome altered proteins was generated. Extensive mining approach employed generate a pathway map highlighting alteration several pathways including calcium signaling, long-term depression, cholesterol Moreover, 17 potential drugs known modes action interact 4 inositol 1,4,5-triphosphate (InsP3) receptors gap junction protein alpha 1 identified. Most likely, these candidates for drug repurposing require further experimental validation.

Language: Английский

Citations

0