Expression and functional analysis of adipokinetic hormone reveal its different roles in larval development and female fecundity in Panonychus citri (McGregor) (Acari: Tetranychidae) DOI

Xinyan Kong,

Ying Xie, Ying Cao

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract Adipokinetic hormone (AKH), a crucial neuropeptide, participates in the important physiological processes by specially binding to its receptor activate AKH signalling pathway. regulates energy metabolism. However, it remains unknown whether affects larval development and adult reproduction influencing In present study, was identified from Panonychus citri contained conserved functional domain ‘Q‐[LIV]‐[NT]‐F‐[ST]‐X (2)‐W’ that characterises family. The relative expression levels of PcAKH revealed different patterns at developmental stages P. . Feeding double‐standard RNA against induced decreased fecundity reduced survival, which accompanied down‐regulation vitellogenin gene expression. addition, after silencing , lipid metabolism carbohydrate homeostasis were disrupted, manifested increased body width weight, fasting phenomenon. Further investigation found compared with control, changes trehalose triglyceride contents variations mRNA genes related disorder may affect female reproduction, lead insufficient deposition. Moreover, seriously affected growth larvae, as delayed period difficulty moulting. Conclusively, all these results current study demonstrated double‐stranded system targeting effectively inhibited disturbing metabolism, is specific RNAi target for control design biopesticide sustainable agriculture.

Language: Английский

Expression and functional analysis of adipokinetic hormone reveal its different roles in larval development and female fecundity in Panonychus citri (McGregor) (Acari: Tetranychidae) DOI

Xinyan Kong,

Ying Xie, Ying Cao

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract Adipokinetic hormone (AKH), a crucial neuropeptide, participates in the important physiological processes by specially binding to its receptor activate AKH signalling pathway. regulates energy metabolism. However, it remains unknown whether affects larval development and adult reproduction influencing In present study, was identified from Panonychus citri contained conserved functional domain ‘Q‐[LIV]‐[NT]‐F‐[ST]‐X (2)‐W’ that characterises family. The relative expression levels of PcAKH revealed different patterns at developmental stages P. . Feeding double‐standard RNA against induced decreased fecundity reduced survival, which accompanied down‐regulation vitellogenin gene expression. addition, after silencing , lipid metabolism carbohydrate homeostasis were disrupted, manifested increased body width weight, fasting phenomenon. Further investigation found compared with control, changes trehalose triglyceride contents variations mRNA genes related disorder may affect female reproduction, lead insufficient deposition. Moreover, seriously affected growth larvae, as delayed period difficulty moulting. Conclusively, all these results current study demonstrated double‐stranded system targeting effectively inhibited disturbing metabolism, is specific RNAi target for control design biopesticide sustainable agriculture.

Language: Английский

Citations

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