Direct quantification of unicellular algae sinking velocities reveals cell size, light, and nutrient-dependence DOI Creative Commons
Teemu P. Miettinen, Annika L. Gomez, Yanqi Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 23, 2023

ABSTRACT Eukaryotic phytoplankton, also known as algae, form the basis of marine food webs and drive carbon sequestration when their biomass sinks to ocean floor. Algae must regulate vertical movement, determined by motility gravitational sinking, balance access light at surface nutrients in deeper layers. However, regulation sinking velocities remains largely unknown, especially motile species. Here, we directly quantify single-cell masses volumes calculate according Stokes’ law diverse clades unicellular microalgae. Our results reveal cell size, light, nutrient-dependency velocities. We identify dinoflagellate green algal species that increase velocity response starvation. Mechanistically, this increased is achieved photosynthesis-driven accumulation carbohydrates, which increases mass density. Moreover, correlate inversely with proliferation rates, mechanism regulating integrates signals from multiple nutrients. findings suggest composition environmental conditions contributes movement cells oceans. More broadly, our approach for measurements expands study supports modeling pump nutrient cycles.

Language: Английский

Recent advances in engineering fast-growing cyanobacterial species for enhanced CO2 fixation DOI Creative Commons
David Kim, José Ángel Moreno-Cabezuelo,

Eduardo Nicolas Schulz

et al.

Frontiers in Climate, Journal Year: 2024, Volume and Issue: 6

Published: June 17, 2024

Atmospheric CO 2 removal (CDR) is a fundamentally endergonic process. Performing CDR or Bioenergy with Carbon Capture and Storage (BECCS) at the gigatonne scale will produce significant additional burden on planet’s limited renewable energy resources irrespective of technology employed. Harnessing photosynthesis to drive industrial-scale fixation has been interest because its minimal requirements potential low costs. In this review, we evaluated thermodynamic considerations performing atmospheric carbon using microalgae cyanobacteria versus physicochemical processes explore implications these energetic costs scalability each respective solution. We review biomass productivities recently discovered fast-growing cyanobacterial strains discuss prospects genetically engineering certain metabolic pathways for channeling fixed into ‘carbon sinks’ further enhance their capture while concurrently extracting value. share our perspectives how new highly productive chassis combined advanced flux balance models, essentially coupling synthetic biology industrial biotechnology, may unlock more favorable methods CDR, both from an economic perspective.

Language: Английский

Citations

4
Cell size, density, and nutrient dependency of unicellular algal gravitational sinking velocities DOI Creative Commons
Teemu P. Miettinen, Annika L. Gomez, Yanqi Wu

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(27)

Published: July 5, 2024

Eukaryotic phytoplankton, also known as algae, form the basis of marine food webs and drive carbon sequestration. Algae must regulate their motility gravitational sinking to balance access light at surface nutrients in deeper layers. However, regulation remains largely unknown, especially motile species. Here, we quantify velocities according Stokes’ law diverse clades unicellular microalgae reveal cell size, density, nutrient dependency velocities. We identify a algal species, Tetraselmis sp., that sinks faster when starved due photosynthesis-driven accumulation carbohydrates loss intracellular water, both which increase density. Moreover, is connected proliferation can respond multiple nutrients. Overall, our work elucidates how size density environmental conditions vertical migration algae.

Language: Английский

Citations

4
Engineering highly productive cyanobacteria towards carbon negative emissions technologies DOI Creative Commons
Angelo J Victoria, Michael J Astbury, Alistair J. McCormick

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103141 - 103141

Published: May 11, 2024

Cyanobacteria are a diverse and ecologically important group of photosynthetic prokaryotes that contribute significantly to the global carbon cycle through capture CO2 as biomass. Cyanobacterial biotechnology could play key role in sustainable bioeconomy negative emissions technologies (NETs), such sequestration or bioproduction. However, primary issues low productivities high infrastructure costs currently limit commercialisation applications. The isolation several fast-growing strains recent advancements molecular biology tools now offer promising new avenues for improving yields, including metabolic engineering approaches guided by high-throughput screening models. Furthermore, emerging research on coculture communities help develop more robust culturing systems support broader NET

Language: Английский

Citations

3
What is holding back cyanobacterial research and applications? A survey of the cyanobacterial research community DOI Creative Commons
Nicolas Schmelling, Moritz Bross

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 8, 2024

Cyanobacteria are a diverse group of prokaryotic organisms that have been the subject intense basic research, resulting in wealth knowledge about fundamental cellular processes such as photosynthesis. However, translation research towards industry-relevant applications is still limited. To understand reasons for this contradictory situation, we conducted quantitative survey among researchers cyanobacterial community, set individual interviews with established researchers, and literature analysis. Our results show community seems to be committed embracing diversity promoting collaboration. Additionally, participants expressed strong desire develop standardized protocols establish larger consortia accelerate progress. The highlight need more integrated approach encompasses both applied aspects. Based on interview well our analysis, areas potential improvement, strategies enhance open questions demand further exploration. Addressing these challenges should development industrial based research.

Language: Английский

Citations

2
Direct quantification of unicellular algae sinking velocities reveals cell size, light, and nutrient-dependence DOI Creative Commons
Teemu P. Miettinen, Annika L. Gomez, Yanqi Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 23, 2023

ABSTRACT Eukaryotic phytoplankton, also known as algae, form the basis of marine food webs and drive carbon sequestration when their biomass sinks to ocean floor. Algae must regulate vertical movement, determined by motility gravitational sinking, balance access light at surface nutrients in deeper layers. However, regulation sinking velocities remains largely unknown, especially motile species. Here, we directly quantify single-cell masses volumes calculate according Stokes’ law diverse clades unicellular microalgae. Our results reveal cell size, light, nutrient-dependency velocities. We identify dinoflagellate green algal species that increase velocity response starvation. Mechanistically, this increased is achieved photosynthesis-driven accumulation carbohydrates, which increases mass density. Moreover, correlate inversely with proliferation rates, mechanism regulating integrates signals from multiple nutrients. findings suggest composition environmental conditions contributes movement cells oceans. More broadly, our approach for measurements expands study supports modeling pump nutrient cycles.

Language: Английский

Citations

1