Delineating the functional activity of antibodies with cross-reactivity to SARS-CoV-2, SARS-CoV-1 and related sarbecoviruses DOI Creative Commons
Felicitas Ruiz, William Foreman, Michelle M. Lilly

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(10), P. e1012650 - e1012650

Published: Oct. 28, 2024

The recurring spillover of pathogenic coronaviruses and demonstrated capacity sarbecoviruses, such SARS-CoV-2, to rapidly evolve in humans underscores the need better understand immune responses this virus family. For purpose, we characterized functional breadth potency antibodies targeting receptor binding domain (RBD) spike glycoprotein that exhibited cross-reactivity against SARS-CoV-2 variants, SARS-CoV-1 sarbecoviruses from diverse clades animal origins with potential. One neutralizing antibody, C68.61, showed remarkable neutralization both variants viruses different sarbecovirus clades. which targets a conserved RBD class 5 epitope, did not select for escape or culture nor have predicted among circulating strains, suggesting epitope is functionally constrained. We identified 11 additional SARS-CoV-2/SARS-CoV-1 cross-reactive target more sequence 4 epitopes within show activity subset one antibody every single tested. A these Fc-mediated effector functions as potent impact infection outcome models. Thus, our study regions across may serve therapeutics pandemic preparedness well blueprints design immunogens capable eliciting cross-neutralizing responses.

Language: Английский

Cryo-electron microscopy in the study of virus entry and infection DOI Creative Commons
Moumita Dutta, Priyamvada Acharya

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: July 24, 2024

Viruses have been responsible for many epidemics and pandemics that impacted human life globally. The COVID-19 pandemic highlighted both our vulnerability to viral outbreaks, as well the mobilization of scientific community come together combat unprecedented threat humanity. Cryo-electron microscopy (cryo-EM) played a central role in understanding SARS-CoV-2 during continues inform about this evolving pathogen. Cryo-EM with its two popular imaging modalities, single particle analysis (SPA) cryo-electron tomography (cryo-ET), has contributed immensely structure viruses interactions define their cycles pathogenicity. Here, we review how cryo-EM informed three distinct viruses, which - HIV-1 infect humans, third, bacteriophages, bacteria. For focus is on surface glycoproteins are mediating host receptor binding, cell membrane fusion, while structure, capsid maturation, attachment bacterial infection initiation mechanism.

Language: Английский

Citations

2
Delineating the functional activity of antibodies with cross-reactivity to SARS-CoV-2, SARS-CoV-1 and related sarbecoviruses DOI Creative Commons
Felicitas Ruiz, William Foreman, Michelle M. Lilly

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(10), P. e1012650 - e1012650

Published: Oct. 28, 2024

The recurring spillover of pathogenic coronaviruses and demonstrated capacity sarbecoviruses, such SARS-CoV-2, to rapidly evolve in humans underscores the need better understand immune responses this virus family. For purpose, we characterized functional breadth potency antibodies targeting receptor binding domain (RBD) spike glycoprotein that exhibited cross-reactivity against SARS-CoV-2 variants, SARS-CoV-1 sarbecoviruses from diverse clades animal origins with potential. One neutralizing antibody, C68.61, showed remarkable neutralization both variants viruses different sarbecovirus clades. which targets a conserved RBD class 5 epitope, did not select for escape or culture nor have predicted among circulating strains, suggesting epitope is functionally constrained. We identified 11 additional SARS-CoV-2/SARS-CoV-1 cross-reactive target more sequence 4 epitopes within show activity subset one antibody every single tested. A these Fc-mediated effector functions as potent impact infection outcome models. Thus, our study regions across may serve therapeutics pandemic preparedness well blueprints design immunogens capable eliciting cross-neutralizing responses.

Language: Английский

Citations

0