Notch Signaling Drives Pro-Regenerative and Migratory Traits in Glandular Stem/Progenitor Cells DOI Open Access
Davide Cinat, Rufina Maturi,

Jeremy Putra Gunawan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Organoid models have advanced our understanding of adult stem/progenitor cell dynamics and function. However, uncovering the regulatory mechanisms scarce often quiescent stem cells in organs like salivary glands remains challenging. Using single-cell RNA sequencing bulk ATAC RNA-sequencing analysis, we conducted in-depth profiling cellular populations key signaling pathways characterizing a mouse submandibular gland organoid (mSGO) model at different temporal stages response to radiation damage. We identified Sox9- Itgb1- expressing as most primitive uncover novel stemness migratory roles for Cd44 -expressing cells. Moreover, revealed that Notch is essential maintaining self-renewal migration potential these post-irradiation. Extending findings patient-derived mSGOs, well murine mammary thyroid organoids, confirmed conserved regulator function under migrative regenerative conditions.

Language: Английский

Potential Benefits of Combining Proton or Carbon Ion Therapy with DNA Damage Repair Inhibitors DOI Creative Commons

Gro Elise Rødland,

Mihaela Temelie,

Adrian Eek Mariampillai

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 1058 - 1058

Published: June 19, 2024

The use of charged particle radiotherapy is currently increasing, but combination therapy with DNA repair inhibitors remains to be exploited in the clinic. high-linear energy transfer (LET) radiation delivered by particles causes clustered damage, which particularly effective destroying cancer cells. Whether damage response this type different from that elicited low-LET radiation, and if how it can targeted increase treatment efficacy, not fully understood. Although several preclinical studies have reported radiosensitizing effects when proton or carbon ion irradiation combined of, e.g., PARP, ATR, ATM, DNA-PKcs, further exploration required determine most treatments. Here, we examine what known about pathway choice high- versus irradiation, discuss these pathways protons ions. Additionally, explore potential on antitumor immune signaling upon irradiation. Due reduced effect healthy tissue better preservation, may well suited for inhibitors.

Language: Английский

Citations

4
Future of Team-based Basic and Translational Science in Radiation Oncology DOI Creative Commons
Robert P. Coppes, Lisanne V. van Dijk

Seminars in Radiation Oncology, Journal Year: 2024, Volume and Issue: 34(4), P. 370 - 378

Published: Sept. 11, 2024

Language: Английский

Citations

1
Interferon signaling is enhanced by ATR inhibition in glioblastoma cells irradiated with X-rays, protons or carbon ions DOI Creative Commons

Gro Elise Rødland,

Mihaela Temelie,

Adrian Eek Mariampillai

et al.

Radiotherapy and Oncology, Journal Year: 2024, Volume and Issue: 203, P. 110669 - 110669

Published: Dec. 13, 2024

Language: Английский

Citations

1
Notch Signaling Drives Pro-Regenerative and Migratory Traits in Glandular Stem/Progenitor Cells DOI Open Access
Davide Cinat, Rufina Maturi,

Jeremy Putra Gunawan

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Organoid models have advanced our understanding of adult stem/progenitor cell dynamics and function. However, uncovering the regulatory mechanisms scarce often quiescent stem cells in organs like salivary glands remains challenging. Using single-cell RNA sequencing bulk ATAC RNA-sequencing analysis, we conducted in-depth profiling cellular populations key signaling pathways characterizing a mouse submandibular gland organoid (mSGO) model at different temporal stages response to radiation damage. We identified Sox9- Itgb1- expressing as most primitive uncover novel stemness migratory roles for Cd44 -expressing cells. Moreover, revealed that Notch is essential maintaining self-renewal migration potential these post-irradiation. Extending findings patient-derived mSGOs, well murine mammary thyroid organoids, confirmed conserved regulator function under migrative regenerative conditions.

Language: Английский

Citations

0