Deciphering seed development and germination in the single-cell era
Seed Science Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 10
Published: March 3, 2025
Abstract
Seeds
are
complex
structures
that
serve
as
dispersal
units
in
angiosperms.
consist
of
three
specialized
tissues
with
distinct
roles
and
molecular
compositions.
Hence,
the
characterization
genetic
regulators
act
within
individual
seed
tissues,
how
their
activity
changes
during
development
germination,
has
been
a
primary
focus
research.
However,
our
knowledge
spatiotemporal
modulation
seeds,
across
different
cell
types,
limited
by
resolution
available
techniques.
In
last
few
years,
application
single-cell
technologies
plants
have
enabled
elucidation
gene
networks
involved
various
developmental
processes
at
cellular
level.
Some
studies
applied
these
to
enabling
further
germination
Here,
we
review
current
status
seeds
present
workflow
for
conducting
transcriptomics.
Additionally,
discuss
integration
multi-omics,
aiming
demonstrate
potential
enhancing
comprehension
regulations
governing
germination.
Language: Английский
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling
Current Opinion in Biotechnology,
Journal Year:
2024,
Volume and Issue:
88, P. 103151 - 103151
Published: May 31, 2024
The
advent
of
gene
editing
technologies
such
as
CRISPR
has
simplified
co-ordinating
trait
development.
However,
identifying
candidate
genes
remains
a
challenge
due
to
complex
networks
and
pathways.
These
exhibit
pleiotropy,
complicating
the
determination
specific
pathway
functions.
In
this
review,
we
explore
how
systems
biology
single-cell
sequencing
can
aid
in
for
specifics
plant
growth
development
within
temporal
tissue
contexts.
Exploring
sequence-function
space
these
modules
with
synthetic
allows
us
test
hypotheses
define
genotype-phenotype
relationships
through
reductionist
approaches.
Collectively,
techniques
hold
potential
advance
breeding
genetic
engineering
strategies
while
also
addressing
diversity
issues
critical
adaptation
Language: Английский
The current economics and throughput of single cell proteomics by liquid chromatography mass spectrometry
Published: June 11, 2024
Single
cell
proteomics
by
mass
spectrometry
(SCP)
is
an
emerging
field
of
study
that
has
captured
the
interest
and
imagination
biologists
in
a
wide
array
disciplines.
In
pursuit
this
new
dizzying
technologies
techniques
have
demonstrated
ability
to
quantify
hundreds
few
thousand
proteins
single
mammalian
cells
typical
size.
One
striking
characteristic
these
methods
range
relative
costs
associated
with
analysis
each
cell.
We
attempted
estimate
cost
per
across
17
different
studies
based
on
quotes
we
obtained
for
hardware,
reagents
instrument
support
plans
relation
number
can
be
analyzed
day.
Before
including
labor
or
facilities,
find
analyze
size
from
less
than
<$2
over
$50
The
increase
appears
directly
related
decrease
throughput
as
measured
theoretical
maximum
Perhaps
most
surprising
observation
average
year.
This
when
compared
emergence
RNA
sequencing
where
increased,
cost/cell
decreased
exponentially
first
7
years
field’s
emergence.
While
made
many
assumptions
obtain
estimates,
hope
will
informative
scientists
interested
obtaining
SCP
data
spectrometrists
who
are
considering
entering
field.
provided
spreadsheet
simple
calculator
supplemental
allow
others
adjust
our
calculations
other
variables
which
inevitably
described
future.
Language: Английский
Exploring the feasibility of a single-protoplast proteomic analysis
Journal of Analytical Science & Technology,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: July 31, 2024
Abstract
Background
Recent
advances
in
high-resolution
mass
spectrometry
have
now
enabled
the
study
of
proteomes
at
single-cell
level,
offering
potential
to
unveil
novel
aspects
cellular
processes.
Remarkably,
there
has
been
no
prior
attempt
investigate
single-plant
cell
proteomes.
In
this
study,
we
aimed
explore
feasibility
conducting
a
proteomic
analysis
on
individual
protoplasts.
Findings
As
result,
our
identified
978
proteins
from
180
protoplasts,
aligning
with
well-known
biological
processes
plant
leaves,
such
as
photosynthetic
electron
transport
photosystem
II.
Employing
SCP
package
SCoPE2
workflow
revealed
notable
batch
effect
and
extensive
missing
values
data.
Following
correction,
observed
heterogeneity
single-protoplast
proteome
expression.
Comparing
results
proteomics
those
bulk
leaf
proteomics,
noted
that
only
small
fraction
data
was
detected
data,
highlighting
technical
limitation
current
method.
Conclusions
summary,
demonstrated
experiment,
revealing
This
underscores
importance
analyzing
substantial
number
cells
discern
statistically
significant
changes
upon
perturbation
abscisic
acid
treatment
future
studies.
We
anticipate
will
contribute
advancing
near
future.
Language: Английский