pH-Controlled chemoselective rapid azo-coupling reaction (CRACR) enables global profiling of serotonylation proteome in cancer cells DOI Open Access
Nan Zhang, Jinghua Wu, Shuaixin Gao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 11, 2024

Serotonylation has been identified as a novel protein post-translational modification for decades, where an isopeptide bond is formed between the glutamine residue and serotonin through transamination. Transglutaminase 2 (also known TGM2 or TGase2) was proven to act main writer enzyme this PTM number of key regulatory proteins (including small GTPases, fibronectin, fibrinogen, transporter, histone H3) have characterized substrates serotonylation. However, due lack pan-specific antibodies serotonylated glutamine, precise enrichment proteomic profiling serotonylation still remain challenging. In our previous research, we developed aryldiazonium probe specifically label in bioorthogonal manner, which depended on pH-controlled chemoselective rapid azo-coupling reaction (CRACR). Here, report application photoactive aryldiazonium-biotin global proteome cancer cells. Thus, over 1,000 were from HCT 116 cells, many are highly related carcinogenesis. Moreover, sites these determined, attributed successful chemical approach. Overall, findings provided new insights into significant association cellular development, further suggesting that target TGM2-mediated monoaminylation may serve promising strategy therapeutics.

Language: Английский

pH-Controlled chemoselective rapid azo-coupling reaction (CRACR) enables global profiling of serotonylation proteome in cancer cells DOI Open Access
Nan Zhang, Jinghua Wu, Shuaixin Gao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 11, 2024

Serotonylation has been identified as a novel protein post-translational modification for decades, where an isopeptide bond is formed between the glutamine residue and serotonin through transamination. Transglutaminase 2 (also known TGM2 or TGase2) was proven to act main writer enzyme this PTM number of key regulatory proteins (including small GTPases, fibronectin, fibrinogen, transporter, histone H3) have characterized substrates serotonylation. However, due lack pan-specific antibodies serotonylated glutamine, precise enrichment proteomic profiling serotonylation still remain challenging. In our previous research, we developed aryldiazonium probe specifically label in bioorthogonal manner, which depended on pH-controlled chemoselective rapid azo-coupling reaction (CRACR). Here, report application photoactive aryldiazonium-biotin global proteome cancer cells. Thus, over 1,000 were from HCT 116 cells, many are highly related carcinogenesis. Moreover, sites these determined, attributed successful chemical approach. Overall, findings provided new insights into significant association cellular development, further suggesting that target TGM2-mediated monoaminylation may serve promising strategy therapeutics.

Language: Английский

Citations

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