Evolution of apoptotic signalling pathways among metazoans: insights from lophotrochozoans DOI Creative Commons
Helen R Horkan, Nikolay Popgeorgiev, Michel Vervoort

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Apoptosis is the main form of regulated cell death in metazoans. Apoptotic pathways are well characterised nematode, fly and mammals, leading to a vision conservation apoptotic However, we recently showed that intrinsic apoptosis fact divergent among In addition, extrinsic poorly studied non-mammalian animals, making its evolution unclear. Consequently, our understanding signalling black-box which must be illuminated by extending research new biological systems. Lophotrochozoans major clade metazoans which, despite their considerable diversity key phylogenetic position as sister group ecdysozoans ( i.e. fly, nematode), explored, especially regarding mechanisms. Traditionally each pathway was considered rely on specific initiator Caspase, associated with an activator. To shed light explored evolutionary history Caspases, Caspase activators BCL-2 family (which control mitochondrial pathway) lophotrochozoans using analysis protein interaction predictions. We discovered diversification Caspases molluscs, annelids brachiopods, loss components platyhelminths, along emergence ankyrin pro-domain. Taken together, data show actors’ lophotrochozoans, further demonstrating appearance distinct during metazoan evolution. Significance statement Apoptosis, programmed death, has been long model organisms such mouse, humans. The restricted focus these models led overall view genes involved highly conserved across all animals. advent next generation sequencing boom omics available tree life. Thanks this, Lophotrochozoa annelids, flatworms, brachiopods), one three large clades make up bilaterian found complex genes, multiple losses, gains, divergences redundancies, highlighting value exploring gene mechanisms Lophotrochozoans.

Language: Английский

The molecular arsenal of the key coastal bioturbator Hediste diversicolor faced with changing oceans DOI Creative Commons
Kaylee Beine, Lauric Feugere, Nichola Fletcher

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract The importance of infaunal bioturbators for the functioning marine ecosystems cannot be overstated. Inhabitants estuarine and coastal habitats are expected to show resilience fluctuations in seawater temperature pH, which adds complexity our understanding effects global change drivers. Further, stress responses may propagated through chemical cues within across species, amplify costs life alter species interactions. Research into molecular mechanisms underlying this has been limited by a lack annotated genomes associated tools. In study, we present first chromosome-level, draft genome ragworm Hediste diversicolor , specifically mapping genes important communication, sensing pH homeostasis. Using these resources, then evaluate transcriptomic behavioural two distinct populations — one field-sampled from Portugal (Ria Formosa) laboratory-acclimated -bred United Kingdom (Humber) changes temperature, odour low pH-stressed predator. Both displayed adaptive future oceanic conditions, with targeted acid-base regulation Ria Formosa population experiment, broader metabolism growth Humber experiment. Chemical stressed fish predators induced related Schreckstoff biosynthesis ragworms. Additionally, under ocean conditions including increased exhibited signs cellular damage. Our findings using new offer novel insights arsenal aids predicting impacts an increasingly acidified unstable ocean, transfer knowledge investigate less tolerance.

Language: Английский

Citations

0
Evolution of apoptotic signalling pathways among metazoans: insights from lophotrochozoans DOI Creative Commons
Helen R Horkan, Nikolay Popgeorgiev, Michel Vervoort

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Apoptosis is the main form of regulated cell death in metazoans. Apoptotic pathways are well characterised nematode, fly and mammals, leading to a vision conservation apoptotic However, we recently showed that intrinsic apoptosis fact divergent among In addition, extrinsic poorly studied non-mammalian animals, making its evolution unclear. Consequently, our understanding signalling black-box which must be illuminated by extending research new biological systems. Lophotrochozoans major clade metazoans which, despite their considerable diversity key phylogenetic position as sister group ecdysozoans ( i.e. fly, nematode), explored, especially regarding mechanisms. Traditionally each pathway was considered rely on specific initiator Caspase, associated with an activator. To shed light explored evolutionary history Caspases, Caspase activators BCL-2 family (which control mitochondrial pathway) lophotrochozoans using analysis protein interaction predictions. We discovered diversification Caspases molluscs, annelids brachiopods, loss components platyhelminths, along emergence ankyrin pro-domain. Taken together, data show actors’ lophotrochozoans, further demonstrating appearance distinct during metazoan evolution. Significance statement Apoptosis, programmed death, has been long model organisms such mouse, humans. The restricted focus these models led overall view genes involved highly conserved across all animals. advent next generation sequencing boom omics available tree life. Thanks this, Lophotrochozoa annelids, flatworms, brachiopods), one three large clades make up bilaterian found complex genes, multiple losses, gains, divergences redundancies, highlighting value exploring gene mechanisms Lophotrochozoans.

Language: Английский

Citations

1