Cited by Enhanced Quadruplet Suppression in <i>E. coli</i> for Expanding the Genetic Code

Cellular Site-Specific Incorporation of Noncanonical Amino Acids in Synthetic Biology DOI

Wei Niu, Jiantao Guo

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(18), P. 10577 - 10617

Published: Aug. 29, 2024

Over the past two decades, genetic code expansion (GCE)-enabled methods for incorporating noncanonical amino acids (ncAAs) into proteins have significantly advanced field of synthetic biology while also reaping substantial benefits from it. On one hand, they provide biologists with a powerful toolkit to enhance and diversify biological designs beyond natural constraints. Conversely, has not only propelled development ncAA incorporation through sophisticated tools innovative strategies but broadened its potential applications across various fields. This Review delves methodological advancements primary site-specific cellular ncAAs in biology. The topics encompass expanding codon addition, creating semiautonomous autonomous organisms, designing regulatory elements, manipulating extending peptide product biosynthetic pathways. concludes by examining ongoing challenges future prospects GCE-enabled highlighting opportunities further this rapidly evolving field.

Language: Английский

Citations

The design and engineering of synthetic genomes DOI