Expansion microscopy reveals neural circuit organization in genetic animal models DOI Creative Commons

Shakila Behzadi,

Jacquelin Ho, Zainab Tanvir

et al.

Neurophotonics, Journal Year: 2024, Volume and Issue: 12(01)

Published: Dec. 20, 2024

Expansion microscopy is a super-resolution technique in which physically enlarging the samples an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This particularly useful neuroscience as many important are smaller than diffraction limit. Since its invention 2015, variety of expansion protocols have been generated and applied to advance knowledge prominent organisms neuroscience, including zebrafish, mice, Drosophila, Caenorhabditis elegans. We review last decade microscopy–enabled advances with focus on neuroscience.

Language: Английский

Feeling the force from within – new tools and insights into nuclear mechanotransduction DOI Creative Commons
Julien Morival,

Anna Hazelwood,

Jan Lammerding

et al.

Journal of Cell Science, Journal Year: 2025, Volume and Issue: 138(5)

Published: March 1, 2025

The ability of cells to sense and respond mechanical signals is essential for many biological processes that form the basis cell identity, tissue development maintenance. This process, known as mechanotransduction, involves crucial feedback between force biochemical signals, including epigenomic modifications establish transcriptional programs. These programs, in turn, reinforce properties its withstand perturbation. nucleus has long been hypothesized play a key role mechanotransduction due direct exposure forces transmitted through cytoskeleton, receiving cytoplasmic central function gene regulation. However, parsing out specific contributions from those surface cytoplasm remains substantial challenge. In this Review, we examine latest evidence on how regulates both via nuclear envelope (NE) epigenetic machinery elements within interior. We also explore establishing memory, characterized by mechanical, transcriptomic state persists after stimuli cease. Finally, discuss current challenges field present technological advances are poised overcome them.

Language: Английский

Citations

1
Ultrastructure expansion microscopy: Enlarging our perspective on apicomplexan cell division DOI Open Access
Sofía Horjales, Florencia Sena, María E. Francia

et al.

Journal of Microscopy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Abstract Apicomplexans, a large phylum of protozoan intracellular parasites, well known for their ability to invade and proliferate within host cells, cause diseases with major health economic impacts worldwide. These parasites are responsible conditions such as malaria, cryptosporidiosis, toxoplasmosis, which affect humans other animals. Apicomplexans exhibit complex life cycles, marked by diverse modes cell division, closely associated pathogenesis. All the unique structural evolutionary characteristics apicomplexan biology underlying stage transitions, singular mechanisms division alongside biomedical relevance have captured attention parasitologists all times. Traditional light electron microscopy set fundamental foundations our understanding these including distinction among division. This has been more recently complemented advances through implementation superresolution fluorescence microscopy, variants cryo‐EM tomography, revealing intricate details organelles Ultrastructure Expansion Microscopy emerged transformative, accessible approach that enhances resolution physically expanding samples isometrically, allowing nanoscale visualisation on standard microscopes. In this work, we review most recent contributions U‐ExM its improvements innovations, in providing unprecedented insights into ultrastructure mechanisms, focusing particularly We highlight power combination protein‐specific labelling, aiding long oversighted detailed assembly parasite‐specific structures, conoid Plasmodia , apical‐basal axis Toxoplasma respectively, during new parasite assembly. Altogether, reveal conserved features across species while nearing super resolution. The development methodologies different technologies crucial advancing mechanistic biology, offering perspectives may facilitate novel therapeutic strategies against apicomplexan‐caused diseases.

Language: Английский

Citations

0
Super-resolving chromatin in its own terms: Recent approaches to portray genomic organization DOI
Manuel Fernández, Maria Pia Cosma, Maria Victoria Neguembor

et al.

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 92, P. 103021 - 103021

Published: March 3, 2025

Language: Английский

Citations

0
‘Phenomenal’ tool sequences DNA and tracks proteins — without cracking cells open DOI

Heidi Ledford

Nature, Journal Year: 2024, Volume and Issue: 634(8035), P. 759 - 760

Published: Oct. 10, 2024

Language: Английский

Citations

2
Expansion microscopy reveals neural circuit organization in genetic animal models DOI Creative Commons

Shakila Behzadi,

Jacquelin Ho, Zainab Tanvir

et al.

Neurophotonics, Journal Year: 2024, Volume and Issue: 12(01)

Published: Dec. 20, 2024

Expansion microscopy is a super-resolution technique in which physically enlarging the samples an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This particularly useful neuroscience as many important are smaller than diffraction limit. Since its invention 2015, variety of expansion protocols have been generated and applied to advance knowledge prominent organisms neuroscience, including zebrafish, mice, Drosophila, Caenorhabditis elegans. We review last decade microscopy–enabled advances with focus on neuroscience.

Language: Английский

Citations

0