How to Study the Mechanobiology of Intestinal Epithelial Organoids? A Review of Culture Supports, Imaging Techniques, and Analysis Methods DOI Creative Commons

L. Magne,

Florian Bugarin, Audrey Ferrand

et al.

Biology of the Cell, Journal Year: 2025, Volume and Issue: 117(4)

Published: April 1, 2025

ABSTRACT Mechanobiology studies how mechanical forces influence biological processes at different scales, both in homeostasis and pathology. Organoids, 3D structures derived from stem cells, are particularly relevant tools for modeling tissues organs vitro. They currently constitute one of the most suitable models mechanobiology studies. This review provides an overview existing or applicable approaches to organoids We first present types culture supports, including hydrogels organ‐on‐chip. then discuss advanced imaging techniques, studying physical properties allowing visualization cellular responses. also describe available observe by microscopy. Finally, we analytical methods, computational biophysical measurement approaches, which facilitate quantification interactions. aims provide comprehensive possible instrumentations, conduct a mechanobiological study on organoids.

Language: Английский

How to Study the Mechanobiology of Intestinal Epithelial Organoids? A Review of Culture Supports, Imaging Techniques, and Analysis Methods DOI Creative Commons

L. Magne,

Florian Bugarin, Audrey Ferrand

et al.

Biology of the Cell, Journal Year: 2025, Volume and Issue: 117(4)

Published: April 1, 2025

ABSTRACT Mechanobiology studies how mechanical forces influence biological processes at different scales, both in homeostasis and pathology. Organoids, 3D structures derived from stem cells, are particularly relevant tools for modeling tissues organs vitro. They currently constitute one of the most suitable models mechanobiology studies. This review provides an overview existing or applicable approaches to organoids We first present types culture supports, including hydrogels organ‐on‐chip. then discuss advanced imaging techniques, studying physical properties allowing visualization cellular responses. also describe available observe by microscopy. Finally, we analytical methods, computational biophysical measurement approaches, which facilitate quantification interactions. aims provide comprehensive possible instrumentations, conduct a mechanobiological study on organoids.

Language: Английский

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