Mechanics and functional consequences of nuclear deformations

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(9), P. 583 - 602

Published: May 5, 2022

Language: Английский

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Mechanical regulation of chromatin and transcription

Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(10), P. 624 - 643

Published: May 23, 2022

Language: Английский

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Fibroblast and myofibroblast activation in normal tissue repair and fibrosis

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(8), P. 617 - 638

Published: April 8, 2024

Language: Английский

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Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture

Cold Spring Harbor Perspectives in Biology, Journal Year: 2021, Volume and Issue: 14(7), P. a041268 - a041268

Published: Aug. 16, 2021

This work reviews nuclear compartments, defined broadly to include distinct structures, bodies, and chromosome domains. It first summarizes original cytological observations before comparing concepts of compartments emerging from microscopy versus genomic approaches then introducing new multiplexed imaging that promise in the future meld both approaches. I discuss how previous models radial distribution chromosomes or binary division genome into A B are now being refined by recognition more complex compartmentalization. The poorly understood question these established maintained is discussed, including through modern perspective phase separation, moving on address possible functions using role speckles modulating gene expression as an example. Finally, review concludes with a discussion questions for this field.

Language: Английский

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Physical Nature of Chromatin in the Nucleus

Cold Spring Harbor Perspectives in Biology, Journal Year: 2021, Volume and Issue: 13(5), P. a040675 - a040675

Published: April 5, 2021

Kazuhiro Maeshima1,2, Shiori Iida1,2 and Sachiko Tamura1 1Genome Dynamics Laboratory, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan 2Department School Life Science, Sokendai (Graduate University for Advanced Studies), Correspondence: kmaeshim{at}nig.ac.jp

Language: Английский

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The role of the dystrophin glycoprotein complex in muscle cell mechanotransduction

Communications Biology, Journal Year: 2022, Volume and Issue: 5(1)

Published: Sept. 27, 2022

Dystrophin is the central protein of dystrophin-glycoprotein complex (DGC) in skeletal and heart muscle cells. connects actin cytoskeleton to extracellular matrix (ECM). Severing link between ECM intracellular has a devastating impact on homeostasis cells, leading range muscular dystrophies. In addition, loss functional DGC leads progressive dilated cardiomyopathy premature death. functions as molecular spring plays critical role maintaining integrity sarcolemma. Additionally, evidence accumulating, linking mechanosignalling, albeit this still less understood. This review article aims at providing an up-to-date perspective its mechanotransduction. We first discuss intricate relationship cell mechanics function, before examining recent research for dystrophin glycoprotein mechanotransduction biomechanical Finally, we current literature map out how signalling intersects with mechanical pathways highlight potential future points intervention, especially focus cardiomyopathies.

Language: Английский

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DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C

Molecular Cell, Journal Year: 2023, Volume and Issue: 83(20), P. 3659 - 3668.e10

Published: Oct. 1, 2023

The integrity of the nuclear envelope (NE) is essential for maintaining structural stability nucleus. Rupture NE has been frequently observed in cancer cells, especially context mechanical challenges, such as physical confinement and migration. However, spontaneous rupture events, without any obvious challenges to cell, have also described. molecular mechanism(s) these events remain be explored. Here, we show that DNA damage subsequent ATR activation leads rupture. Upon damage, lamin A/C phosphorylated an ATR-dependent manner, leading changes lamina assembly and, ultimately, In addition, cells with intrinsic repair defects undergo frequent DNA-damage-induced rupture, which renders them extremely sensitive further perturbations. Exploiting this vulnerability could provide a new angle complement traditional, DNA-damage-based chemotherapy.

Language: Английский

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Mechanobiology of organelles: illuminating their roles in mechanosensing and mechanotransduction

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 33(12), P. 1049 - 1061

Published: May 24, 2023

Language: Английский

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Mitochondrial mechanotransduction through MIEF1 coordinates the nuclear response to forces

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(12), P. 2046 - 2060

Published: Oct. 21, 2024

Tissue-scale architecture and mechanical properties instruct cell behaviour under physiological diseased conditions, but our understanding of the underlying mechanisms remains fragmentary. Here we show that extracellular matrix stiffness, spatial confinements applied forces, including stretching mouse skin, regulate mitochondrial dynamics. Actomyosin tension promotes phosphorylation elongation factor 1 (MIEF1), limiting recruitment dynamin-related protein (DRP1) at mitochondria, as well peri-mitochondrial F-actin formation fission. Strikingly, fission is also a general mechanotransduction mechanism. Indeed, found DRP1- MIEF1/2-dependent required sufficient to three transcription factors broad relevance-YAP/TAZ, SREBP1/2 NRF2-to control proliferation, lipogenesis, antioxidant metabolism, chemotherapy resistance adipocyte differentiation in response cues. This extends liver, where DRP1 regulates hepatocyte proliferation identity-hallmark YAP-dependent phenotypes. We propose mitochondria fulfil unifying signalling function by which tissue microenvironment coordinates complementary functions.

Language: Английский

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Principles and functions of pericentromeric satellite DNA clustering into chromocenters

Seminars in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 128, P. 26 - 39

Published: Feb. 8, 2022

Language: Английский

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