Journal of Microscopy and Ultrastructure, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 9, 2025
Language: Английский
Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 2, 2024
Language: Английский
Citations
42
Journal of Biomedical Science, Journal Year: 2025, Volume and Issue: 32(1)
Published: Jan. 9, 2025
Abstract Research into cancer treatment has been mainly focused on developing therapies to directly target cells. Over the past decade, extensive studies have revealed critical roles of tumour microenvironment (TME) in initiation, progression, and drug resistance. Notably, cancer-associated fibroblasts (CAFs) emerged as one primary contributors shaping TME, creating a favourable environment for development. Many preclinical identified promising targets CAFs, demonstrating remarkable efficacy some CAF-targeted treatments models. Encouraged by these compelling findings, therapeutic strategies now advanced clinical evaluation. We aim provide comprehensive review relevant subjects including CAF-related markers targets, their multifaceted roles, current landscape ongoing trials. This knowledge can guide future research CAFs advocate investigations targeting CAFs.
Language: Английский
Citations
7
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 10, 2025
For idiopathic pulmonary fibrosis (IPF), interleukin 11 (IL-11) is a pivotal cytokine that stimulates the transformation of fibroblasts into myofibroblasts, thus accelerating progression fibrosis. Here, we develop an innovative inhalable small interfering RNA (siRNA) delivery system termed PEI-GBZA, which demonstrates impressive efficiency in loading siIL-11 targeting IL-11 (siIL-11) and substantially suppresses differentiation myofibroblasts epithelial-mesenchymal transition (EMT), reduces neutrophil macrophage recruitment, ultimately relieves established fibrotic lesions IPF model. PEI-GBZA prepared by modifying low-molecular-weight polyethylenimine (PEI) with 4-guanidinobenzoic acid (GBZA). The resulting may effectively encapsulate through variety interactions such as hydrophobic, hydrogen bonding, electrostatic interactions, creating stable carrier/siIL-11 nanoparticles (PEI-GBZA/siIL-11 NPs). Upon inhalation, PEI-GBZA/siIL-11 NPs demonstrate effective retention lesions, leading to marked mitigation disease bleomycin-induced Impressively, this inhalation therapy exhibits negligible systemic toxicity. This work provides universal noninvasive therapeutic platform holds significant promise for respiratory diseases. potential clinical application substantial, offering frontier treatment potentially other disorders.
Language: Английский
Citations
4
Regenerative Biomaterials, Journal Year: 2025, Volume and Issue: 12
Published: Jan. 1, 2025
Abstract This study investigates a novel pH-responsive hydrogel composed of polyvinyl alcohol (PVA) and boric acid (BA) designed for the controlled release salvianolic B (SAB), addressing critical challenge scar formation skin regeneration. The dual-crosslinked network architecture exhibits remarkable pH sensitivity, enabling it to achieve peak SAB within 48 hours in acidic microenvironment characteristic early-stage wound healing. In vitro assessments demonstrated that PVA-BA-SAB significantly inhibits fibroblast activation mitigates abnormal collagen deposition, effectively preventing excessive formation. Transcriptome sequencing reveals potential role balancing TGF-β Wnt signaling pathways. Furthermore, vivo studies revealed enhanced tissue regeneration, characterized by improved organization increased vascularization, as well promotion mature hair follicle development. hydrogel’s biocompatibility, mechanical robustness adhesive properties were also thoroughly evaluated, confirming its suitability clinical applications. These findings suggest fully exerts excellent characteristics biomaterials maximizes pharmacological effect SAB. Our innovative drug delivery system not only facilitates healing but offers strategic approach minimize scarring. research provides valuable insights into therapeutic strategies effective management repair.
Language: Английский
Citations
2
Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)
Published: March 28, 2025
Cancer-associated fibroblasts (CAFs) are key players in cancer development and therapy, they exhibit multifaceted roles the tumor microenvironment (TME). From their diverse cellular origins, CAFs undergo phenotypic functional transformation upon interacting with cells presence can adversely influence treatment outcomes severity of cancer. Emerging evidence from single-cell RNA sequencing (scRNA-seq) studies have highlighted heterogeneity plasticity CAFs, subtypes identifiable through distinct gene expression profiles properties. multiple mechanisms, including regulation extracellular matrix (ECM) remodeling, direct promotion growth provision metabolic support, promoting epithelial-mesenchymal transition (EMT) to enhance invasiveness growth, as well stimulating stem cell properties within tumor. Moreover, induce an immunosuppressive TME contribute therapeutic resistance. In this review, we summarize fundamental knowledge recent advances regarding focusing on sophisticated potential targets. We discuss various strategies target ECM modulation, elimination, interruption CAF-TME crosstalk, CAF normalization, approaches developing more effective treatments. An improved understanding complex interplay between is crucial for new targeted therapies
Language: Английский
Citations
2
Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 1, 2024
Helicobacter pylori (H. pylori) infection is the main risk for gastric cancer (GC). However, cellular heterogeneity and underlying molecular mechanisms in H. pylori-driven tumorigenesis are poorly understood.
Language: Английский
Citations
11
Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 10, 2025
Burn care and treatment differ markedly from other types of wounds, as they are significantly more prone to infections struggle maintain fluid balance post-burn. Moreover, the limited self-healing abilities exacerbate likelihood scar formation, further complicating recovery process. To tackle these issues, an asymmetric wound dressing comprising a quercetin-loaded poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB@Qu) hydrophilic layer zinc oxide nanoparticle-loaded, thermally treated polyvinylidene fluoride (HPVDF@ZnO) hydrophobic is designed. This provided antibacterial property exudate management in early stages burn treatment, preventing infection maintaining moisture at site. As healing progresses, electroactive properties HPVDF@ZnO quercetin P34HB@Qu synergistically regulate cell migration differentiation, accelerating facilitating scar-free regeneration. Furthermore, assisted regeneration skin appendages. study underscores full-cycle strategy versatile dressings for spatiotemporal injury scarless healing.
Language: Английский
Citations
1
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 13, 2025
Background Fibrotic skin disease represents a major global healthcare burden, characterized by fibroblast hyperproliferation and excessive accumulation of extracellular matrix components. The immune cells are postulated to exert pivotal role in the development fibrotic disease. Single-cell RNA sequencing has been used explore composition functionality present diseases. However, these studies detected gene expression all diseases did not enrich cells. Thus, precise cell atlas remains unknown. In this study, we plan investigate intricate cellular landscape keloid, paradigm Methods CD45 + were enriched fluorescence-activated sorting. was analyze keloid normal scar tissues. Ki-67 staining, scratch experiment, real-time PCR, Western blotting effect Th17 supernatant on fibroblasts. Results Our findings revealed We found that percentage significantly increased keloids compared scars. All subclusters macrophages dendritic (DCs) showed similar proportions between samples samples. upregulated genes M1 macrophages, M2 cDC2 associated with MHC class II protein complex assembly antigen assembly, indicating active keloids. Functional suggested could promote proliferation, collagen expression, migration fibroblasts through interleukin 17A. Importantly, also other diseases, such as hypertrophic scars scleroderma, suggesting broad mechanism for fibrosis. Conclusion summary, built single-cell study. addition, explored function cell-fibroblast interaction These will help understand pathogenesis depth identify potential targets treatment.
Language: Английский
Citations
1
Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
Abstract The processes of skin wound healing and scar formation are complex, often involving hypoxia inflammation, which create a pathological microenvironment that impedes normal healing. Improving oxygenation reducing inflammation crucial for accelerating scarring. Traditional dressings like sponges, gauze, hydrogels struggle to balance moisture retention, breathability, exudate absorption. To address these challenges, rod‐shaped microgel scaffolds with larger surface areas interconnected porous structures explored enhance gas transport, promoting thus Nanoparticles (NPs) used mediate the microgels‐assembled scaffold load catalase (CAT) enhanced bioactivity. In vitro experiments showed this material alleviated oxidative stress reduced activity hypoxia‐inducible factor‐1α (HIF‐1α), nuclear factor kappa B (NF‐κB), downstream transforming growth factor‐β1 (TGF‐β)/Smad pathway in fibroblasts. incorporation CAT significant promotion M2‐phenotype macrophage polarization. vivo studies on rat rabbit wounds demonstrated significantly improved absorption maintaining moist oxygenated environment. These tissue hypoxia, accelerated healing, decreased hypertrophic vivo. This innovative method leveraged unique properties microgels effectively regeneration.
Language: Английский
Citations
1
Biogerontology, Journal Year: 2025, Volume and Issue: 26(1)
Published: Jan. 20, 2025
Language: Английский
Citations
1