The two-component regulatory systems GraRS and SrrAB mediate Staphylococcus aureus susceptibility to Pep5 produced by clinical isolate of Staphylococcus epidermidis DOI
Y Suzuki, Miki Kawada‐Matsuo, Mi Nguyen‐Tra Le

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(7)

Published: June 4, 2024

ABSTRACT Staphylococcus aureus is a common bacterium on the skin and in nose that sometimes causes severe illness. Bacteriocins, antimicrobial peptides, or proteins produced by bacteria are candidates for treatment of S. infection. In this study, we found clinical epidermidis strain, KSE112, lantibiotic Pep5, which showed anti- activity. The complete nucleotide sequence Pep5-encoding plasmid was determined. Several two-component regulatory systems (TCSs) known to be involved bacteriocin susceptibility. Therefore, susceptibility tests were performed using TCS-inactivated mutants determine TCS responsible Pep5 susceptibility; Δ graRS mutant exhibited increased while srrAB decreased GraRS regulate dltABCD mprF concert with vraFG , significantly dltABCD, mprF, mutants. Regarding mutant, cross-resistance aminoglycosides observed. As aminoglycoside activity affected aerobic respiration, focused qoxABCD cydAB, quinol oxidase genes necessary respiration have downregulated expression mutant. We constructed cydAB inactivation aminoglycosides. These results indicate reduced due IMPORTANCE emergence drug-resistant bacteria, including MRSA, health problem worldwide. Thus, development novel agents, bacteriocins, needed. report, Pep5-producing strain S . determined first time. However, aureus, its effectors conferred Pep5. also revealed another TCS, SrrAB, affects other lantibiotics controlling respiration. our investigated efficacy against Gram-positive respiratory constancy regulated required nisin, nukacin, findings provide important information application bacteriocins suggest they different properties among similar pore-forming lantibiotics.

Language: Английский

Oral colonization of antimicrobial-resistant bacteria in home health care participants and their association with oral and systemic status DOI Creative Commons

Saki Nishihama,

Miki Kawada‐Matsuo, Mi Nguyen‐Tra Le

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 17, 2025

Language: Английский

Citations

0
Differences in the antibacterial efficacy of honey against oral and non-oral bacterial pathogens in artificial human saliva DOI Creative Commons

Jana Godočı́ková,

Maria Hrnciarova,

Juraj Deglovič

et al.

European Food Research and Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients DOI Creative Commons

Tomoki Kawayanagi,

Miki Kawada‐Matsuo,

Satoru Kusaka

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 16, 2025

Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it important determine prevalence of community and conduct epidemiological studies from genetic statistical perspectives. In this study, aureus/MRSA was isolated oral nasal cavities 504 dental patients (65 inpatients 439 outpatients). Sixty-two strains 9 were cavity, 112 21 cavity. Multi-locus sequence typing (MLST) analysis showed ST8 high (18 isolates) among 30 isolates, whereas 144 methicillin sensitive ST15 (25 (20 high. Statistical patients' clinical status revealed correlation between denture use. Among 34 whom both sites, 25 had same ST, 23 below 40 single-nucleotide polymorphisms which considered be identical strains. Our study properties as commensals.

Language: Английский

Citations

0
Ursoricin, a bacteriocin of Streptococcus ursoris , has potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci DOI
Chutian Wang, Mi Nguyen‐Tra Le, Miki Kawada‐Matsuo

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(6)

Published: May 22, 2024

The emergence of drug-resistant bacteria, particularly methicillin-resistant

Language: Английский

Citations

2
The two-component regulatory systems GraRS and SrrAB mediate Staphylococcus aureus susceptibility to Pep5 produced by clinical isolate of Staphylococcus epidermidis DOI
Y Suzuki, Miki Kawada‐Matsuo, Mi Nguyen‐Tra Le

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(7)

Published: June 4, 2024

ABSTRACT Staphylococcus aureus is a common bacterium on the skin and in nose that sometimes causes severe illness. Bacteriocins, antimicrobial peptides, or proteins produced by bacteria are candidates for treatment of S. infection. In this study, we found clinical epidermidis strain, KSE112, lantibiotic Pep5, which showed anti- activity. The complete nucleotide sequence Pep5-encoding plasmid was determined. Several two-component regulatory systems (TCSs) known to be involved bacteriocin susceptibility. Therefore, susceptibility tests were performed using TCS-inactivated mutants determine TCS responsible Pep5 susceptibility; Δ graRS mutant exhibited increased while srrAB decreased GraRS regulate dltABCD mprF concert with vraFG , significantly dltABCD, mprF, mutants. Regarding mutant, cross-resistance aminoglycosides observed. As aminoglycoside activity affected aerobic respiration, focused qoxABCD cydAB, quinol oxidase genes necessary respiration have downregulated expression mutant. We constructed cydAB inactivation aminoglycosides. These results indicate reduced due IMPORTANCE emergence drug-resistant bacteria, including MRSA, health problem worldwide. Thus, development novel agents, bacteriocins, needed. report, Pep5-producing strain S . determined first time. However, aureus, its effectors conferred Pep5. also revealed another TCS, SrrAB, affects other lantibiotics controlling respiration. our investigated efficacy against Gram-positive respiratory constancy regulated required nisin, nukacin, findings provide important information application bacteriocins suggest they different properties among similar pore-forming lantibiotics.

Language: Английский

Citations

0