Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates

Biotechnology Advances, Journal Year: 2021, Volume and Issue: 47, P. 107697 - 107697

Published: Jan. 25, 2021

Language: Английский

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The Heat Shock Response in Yeast Maintains Protein Homeostasis by Chaperoning and Replenishing Proteins

Cell Reports, Journal Year: 2019, Volume and Issue: 29(13), P. 4593 - 4607.e8

Published: Dec. 1, 2019

Highlights•The HSR is modular and tuned to the severity of stress•90% upregulation under stress required keep protein levels constant•Protein loss replenished by translation•Aggregation processes shape sublethal heat responseSummaryLife resilient because living systems are able respond elevated temperatures with an ancient gene expression program called shock response (HSR). In yeast, transcription hundreds genes upregulated at temperatures. Besides protection conferred chaperones, function majority has remained enigmatic. We show that those directly counterbalance increased turnover maintain metabolism. This anaplerotic reaction together molecular chaperones allows yeast efficiently buffer proteotoxic stress. When capacity this system exhausted extreme temperatures, aggregation stop translation growth pauses. The emerging concept distinct programs dependent on stress.Graphical abstract

Language: Английский

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Impact of bioethanol impurities on steam reforming for hydrogen production: A review

International Journal of Hydrogen Energy, Journal Year: 2020, Volume and Issue: 45(21), P. 11923 - 11942

Published: March 18, 2020

Language: Английский

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Global patterns and rates of habitat transitions across the eukaryotic tree of life

Nature Ecology & Evolution, Journal Year: 2022, Volume and Issue: 6(10), P. 1458 - 1470

Published: Aug. 4, 2022

Abstract The successful colonization of new habitats has played a fundamental role during the evolution life. Salinity is one strongest barriers for organisms to cross, which resulted in distinct marine and non-marine (including both freshwater soil) communities. Although microbes represent by far vast majority eukaryote diversity, salt barrier shaping diversity across eukaryotic tree poorly known. Traditional views suggest rare ancient marine/non-marine transitions but this view being challenged discovery several recently transitioned lineages. Here, we investigate habitat eukaryotes using unique set taxon-rich phylogenies inferred from combination long-read short-read environmental metabarcoding data spanning ribosomal DNA operon. Our results show that, overall, microbial communities are phylogenetically have occurred directions almost all major lineages, with hundreds transition events detected. Some groups experienced relatively high rates transitions, most notably fungi crossing probably been an important aspect their diversification. At deepest phylogenetic levels, ancestral reconstruction analyses that may first evolved two largest known assemblages (TSAR Amorphea) arose different habitats. Overall, our findings indicate provide global perspective on domain

Language: Английский

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Yeast osmoregulation – glycerol still in pole position

FEMS Yeast Research, Journal Year: 2022, Volume and Issue: 22(1)

Published: Jan. 1, 2022

ABSTRACT In response to osmotic dehydration cells sense, signal, alter gene expression, and metabolically counterbalance differences. The main compatible solute/osmolyte that accumulates in yeast is glycerol, which produced from the glycolytic intermediate dihydroxyacetone phosphate. This review covers recent advancements understanding mechanisms involved sensing, signaling, cell-cycle delays, transcriptional responses as well post-translational modifications on key proteins osmoregulation. protein kinase Hog1 a key-player many of these events, however, there also growing body evidence for important Hog1-independent playing vital roles. Several missing links our osmoregulation will be discussed future avenues research proposed. highlights this rather simple experimental system—salt/sorbitol yeast—has developed into an enormously potent model system unravelling fundamental aspects biology.

Language: Английский

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Microbial Community Affects Daqu Quality and the Production of Ethanol and Flavor Compounds in Baijiu Fermentation

Foods, Journal Year: 2023, Volume and Issue: 12(15), P. 2936 - 2936

Published: Aug. 2, 2023

Daqu is a traditional starter for Baijiu fermentation and produced by spontaneous of ground moistened barley or wheat. The quality traditionally evaluated based on physicochemical subjective sensory parameters without microbiological analysis. Here, we compared the characteristics qualified (QD) inferior (ID) Daqu, their microbial communities plate counting PacBio SMRT sequencing rRNA gene libraries, impacts fermentation. results showed that glucoamylase α-amylase activities QD were significantly higher than those ID. counts yeasts relative abundances functional microbes, especially amylolytic bacterium

Language: Английский

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Bioleaching for metals removal from mine tailings flotation fractions

Hydrometallurgy, Journal Year: 2024, Volume and Issue: 225, P. 106286 - 106286

Published: Feb. 20, 2024

This study investigated two bioleaching strategies for removing heavy metals from three mine tailings fractions generated by flotation processes. On the one hand, with microbial consortia of acidophilic mesophiles and moderate thermophiles efficiently extracted Co, Cu, Zn, As, while leaching Pb was facilitated through use organic acids produced a heterotrophic bacterium fungus. Approximately 100% 68% 63% 31% Cu were bioleached barite (BT) sample after 14 days, whereas concentrate (BC) results showed about 70% Zn 45% removal at same period. The sulfide (SC) underwent both consortia, over 28 days. Approximately, 67% 28% 56% 6% Mn using mesophiles, efficiency 72% 50% 36% 5% in 20 L bioreactors. other explored Gluconobacter oxydans fungus Penicillium simplicissimum production gluconic acid citric acid, respectively. Additionally, besides glucose-based media, glycerol crystal sugar tested as alternative cheaper carbon sources. metabolic activity P. allowed maximum 39–43% BT days glycerol-based medium, BC sample, extraction around 60% medium. A lower achieved G. samples. 34% 39% reached within 7 when glucose used source. Further optimization should address enhancement – especially reduction costs related to media formulation fungal biomass on larger scale.

Language: Английский

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Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage

eLife, Journal Year: 2018, Volume and Issue: 7

Published: April 6, 2018

Fructophily is a rare trait that consists of the preference for fructose over other carbon sources. Here, we show in yeast lineage (the Wickerhamiella/Starmerella, W/S clade) comprised fructophilic species thriving high-sugar floral niche, acquisition fructophily concurrent with wider remodeling central metabolism. Coupling comparative genomics biochemical and genetic approaches, gathered ample evidence loss alcoholic fermentation an ancestor clade subsequent reinstatement through either horizontal homologous bacterial genes or modification pre-existing gene. An enzyme required sucrose assimilation was also acquired from bacteria, suggesting novelties identified may be related to adaptation environment. This work shows how even metabolism can remodeled by surge HGT events.

Language: Английский

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Polyol production from waste materials by genetically modified Yarrowia lipolytica

Bioresource Technology, Journal Year: 2017, Volume and Issue: 243, P. 393 - 399

Published: June 27, 2017

Language: Английский

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Glycerol as a substrate for Saccharomyces cerevisiae based bioprocesses – Knowledge gaps regarding the central carbon catabolism of this ‘non-fermentable’ carbon source

Biotechnology Advances, Journal Year: 2019, Volume and Issue: 37(6), P. 107378 - 107378

Published: March 28, 2019

Glycerol is an interesting alternative carbon source in industrial bioprocesses due to its higher degree of reduction per atom compared sugars. During the last few years, significant progress has been made improving well-known platform organism Saccharomyces cerevisiae with regard glycerol utilization capability, particularly synthetic medium. This provided a basis for future metabolic engineering focusing on production valuable chemicals from glycerol. However, profound knowledge about central catabolism medium prerequisite such incentives. As matter fact, current assumptions actual vivo fluxes active as sole have mainly based omics data collected complex media or were even deduced studies other non-fermentable sources, ethanol acetate. A number uncertainties identified which role glyoxylate cycle, subcellular localization respective enzymes, contributions mitochondrial transporters and anaplerotic reactions under these conditions. The review scrutinizes knowledge, highlights necessity collect novel experimental using cells growing summarizes state art fermentation products that considered so far 'non-fermentable' yeast S. cerevisiae.

Language: Английский

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