Metabolic engineering in the host Yarrowia lipolytica

Metabolic Engineering, Journal Year: 2018, Volume and Issue: 50, P. 192 - 208

Published: July 26, 2018

Language: Английский

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Yeast Protein as an Easily Accessible Food Source

Metabolites, Journal Year: 2022, Volume and Issue: 12(1), P. 63 - 63

Published: Jan. 11, 2022

In recent years, the awareness and willingness of consumers to consume healthy food has grown significantly. order meet these needs, scientists are looking for innovative methods production, which is a source easily digestible protein with balanced amino acid composition. Yeast biomass (single cell protein, SCP) bioavailable product obtained when primarily using as culture medium inexpensive various waste substrates including agricultural industrial wastes. With growing population, yeast seems be an attractive alternative traditional sources such plants meat. Moreover, also contains trace minerals vitamins B-group. Thus, in production provides both valuable nutrients enhances purification conclusion, nutritional may best option human animal nutrition low environmental footprint. The rapidly evolving SCP technology discoveries from world biotechnology can make huge difference future key improvement hunger problems possibility improving security. On market demand cheap environmentally clean practically unlimited scale it soon become one ingredients our food. review article presents possibilities by groups use well safety used

Language: Английский

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Recent advances in biological production of erythritol

Critical Reviews in Biotechnology, Journal Year: 2017, Volume and Issue: 38(4), P. 620 - 633

Published: Sept. 27, 2017

Erythritol is a natural sweetener commonly used in the food and pharmaceutical industries. Produced by microorganisms as an osmoprotectant, it ideal sucrose substitute for diabetics or overweight persons due to its almost zero calorie content. Currently, erythritol produced on industrial scale through fermentation of sugars some yeasts, such Moniliella sp. However, popularity still small because high retail price. This creates opportunity further process improvement. Recent years have brought rapid development biosynthesis methods from low-cost substrates, better understanding metabolic pathways leading synthesis. The yeast Yarrowia lipolytica emerges organism effectively producing pure crude glycerol. Moreover, novel organisms substrates may be taken into considerations engineering. review focuses modification production use engineering order improve yield productivity.

Language: Английский

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Synthetic Biology Expands the Industrial Potential of Yarrowia lipolytica

Trends in biotechnology, Journal Year: 2018, Volume and Issue: 36(10), P. 1085 - 1095

Published: June 4, 2018

Language: Английский

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Erythritol as sweetener—wherefrom and whereto?

Applied Microbiology and Biotechnology, Journal Year: 2017, Volume and Issue: 102(2), P. 587 - 595

Published: Dec. 1, 2017

Erythritol is a naturally abundant sweetener gaining more and importance especially within the food industry. It widely used as in calorie-reduced food, candies, or bakery products. In research focusing on sugar alternatives, erythritol key issue due to its, compared other polyols, challenging production. cannot be chemically synthesized commercially worthwhile way resulting switch biotechnological this area, efforts have been made improve concentration, productivity, yield. This mini review will give an overview attempts production well their development over time.

Language: Английский

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Aseptic production of citric and isocitric acid from crude glycerol by genetically modified Yarrowia lipolytica

Bioresource Technology, Journal Year: 2018, Volume and Issue: 271, P. 340 - 344

Published: Sept. 23, 2018

Language: Английский

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Genetic and bioprocess engineering to improve squalene production in Yarrowia lipolytica

Bioresource Technology, Journal Year: 2020, Volume and Issue: 317, P. 123991 - 123991

Published: Aug. 10, 2020

Squalene is the precursor for triterpene-based natural products and steroids-based drugs. It has been widely used as pharmaceutical intermediates personal care products. The aim of this work to test feasibility engineering Yarrowia lipolytica a potential host squalene production. bottleneck pathway was removed by overexpressing native HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase. With recycling NADPH from mannitol cycle, engineered strain produced about 180.3 mg/L 188.2 glucose or acetate minimal media. By optimizing C/N ratio, controlling media pH mitigating acetyl-CoA flux competition lipogenesis, 502.7 squalene, 28-fold increase over parental (17.2 mg/L). This may serve baseline harness Y. an oleaginous cell factory sustainable production terpenoids-based chemicals

Language: Английский

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Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

Microorganisms, Journal Year: 2020, Volume and Issue: 8(4), P. 574 - 574

Published: April 15, 2020

Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply applications in agro-food, chemical pharmaceutical industries. The main challenge their production is to reach productivity threshold allow process be profitable. This relies on construction efficient cell factories by metabolic engineering development low-cost processes using industrial wastes or cheap widely available raw materials as feedstock. non-conventional yeast Yarrowia lipolytica has emerged recently potential producer such metabolites owing its low nutritive requirements, ability grow at high densities bioreactor ease genome edition. review will focus current knowledge synthesis most important sugar Y. lipolytica.

Language: Английский

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Yarrowia lipolytica as an Alternative and Valuable Source of Nutritional and Bioactive Compounds for Humans

Molecules, Journal Year: 2022, Volume and Issue: 27(7), P. 2300 - 2300

Published: April 1, 2022

Yarrowia lipolytica, an oleagineous species of yeast, is a carrier various important nutrients. The biomass this yeast extensive source protein, exogenous amino acids, bioavailable essenctial trace minerals, and lipid compounds as mainly unsaturated fatty acids. also contains B vitamins, including vitamin B12, many other bioactive components. Therefore, Y. lipolytica can be used in food supplements for humans safe nutritional additives maintaining the homeostasis organism, vegans vegetarians, athletes, people after recovery, at risk deficiencies.

Language: Английский

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Biotechnological valorization of biodiesel-derived glycerol: Trials with the non-conventional yeasts Yarrowia lipolytica and Rhodosporidium sp

Carbon Resources Conversion, Journal Year: 2021, Volume and Issue: 4, P. 61 - 75

Published: Jan. 1, 2021

Four non-conventional yeast strains belonging to the genera Yarrowia and Rhodosporidium were cultivated under nitrogen-limited conditions in shake flasks with biodiesel-derived glycerol employed as sole substrate. The R. toruloides DSM 4444 Y. lipolytica ACA-DC 5033 displayed better performances further studied. At initial (Gly0) ≈ 50 g/L, strain produced dry cell weight (DCW) ≈7.0 also secondary extra-cellular metabolites citric acid (CA) at ≈16 g/L polyols (Pol) (mannitol, erythritol arabitol) ≈21 g/L. In double-media (besides nitrogen Mg was limiting) for Gly0 a shift towards CA production occurred (CAmax 33 g/L). DCW = 18.1 containing lipids 30.3% DCW. single media 90 Pol 48 20 while 27.3 lipid 54.5% w/w. late growth phases, re-consumed. Balanced phase (trophophase) of metabolite synthesis (idiophase) successfully simulated aid modified Velhlust-Aggelis model. Lipid extraction process studied these two by using different methods. Yeast contained mostly oleic acid, constituting suitable precursors 2nd generation biodiesel.

Language: Английский

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