Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 215, P. 114559 - 114559

Published: July 13, 2022

Language: Английский

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Argonaute-triggered visual and rebuilding-free foodborne pathogenic bacteria detection

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 454, P. 131485 - 131485

Published: April 25, 2023

Language: Английский

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CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e26179 - e26179

Published: Feb. 1, 2024

CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.

Language: Английский

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CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: 34, P. 103625 - 103625

Published: April 4, 2024

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.

Language: Английский

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Nanotechnology-leveraged nucleic acid amplification for foodborne pathogen detection

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 506, P. 215745 - 215745

Published: Feb. 21, 2024

Language: Английский

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New Frontiers in CRISPR: Addressing Antimicrobial Resistance with Cas9, Cas12, Cas13, and Cas14

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e42013 - e42013

Published: Jan. 1, 2025

The issue of antimicrobial resistance (AMR) poses a major challenge to global health, evidenced by alarming mortality predictions and the diminishing efficiency conventional drugs. CRISPR-Cas system has proven be powerful tool in addressing this challenge. It originated from bacterial adaptive immune mechanisms gained significant recognition scientific community. This review aims explore applications technologies combating AMR, evaluating their effectiveness, challenges, potential for integration into current strategies. We conducted comprehensive recent literature databases such as PubMed Web Science, focusing on studies that employ against AMR. offer transformative approach combat with extend beyond traditional Integrating these existing methods could significantly enhance our ability manage potentially reverse growing problem resistance. Future research should address technical ethical barriers facilitate safe effective clinical environmental applications. underscores necessity interdisciplinary collaboration international cooperation harness full fight superbugs.

Language: Английский

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A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 443, P. 130234 - 130234

Published: Oct. 27, 2022

Language: Английский

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Comparison of CRISPR/Cas and Argonaute for nucleic acid tests

Trends in biotechnology, Journal Year: 2022, Volume and Issue: 41(5), P. 595 - 599

Published: Dec. 7, 2022

Language: Английский

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Sensitive Small Molecule Aptasensing based on Hybridization Chain Reaction and CRISPR/Cas12a Using a Portable 3D-Printed Visualizer

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(3), P. 1076 - 1084

Published: Jan. 18, 2023

Next-generation biosensing tools based on CRISPR/Cas have revolutionized the molecular detection. A number of CRISPR/Cas-based biosensors been reported for detection nucleic acid targets. The establishment efficient methods non-nucleic target would further broaden scope this technique, but up to now, concerning research is limited. In current study, we a versatile platform small-molecule called SMART-Cas12a (small-molecule aptamer regulated test using CRISPR/Cas12a). Simply, hybridization chain reaction cascade signal amplification was first trigged by functional (aptamer) through binding. Then, system integrated recognize amplified products followed activation trans-cleavage. As such, can be ingeniously converted signals and then fluorescent that readily visualized analyzed customized 3D-printed visualizer with help home-made App-enabled smartphone. Adenosine triphosphate selected as model target, under optimized conditions, achieved fine analytical performance linear range from 0.1 750 μM limit 1.0 nM. satisfactory selectivity recoveries obtained demonstrated method suitable complex sample environment. sample-to-answer time less than 100 min. Our work not only expanded reach CRISPR-Cas in also provided prototype generalized detecting wider analytes desirable adaptability, sensitivity, specificity, on-site capability.

Language: Английский

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CRISPR-based biosensors for pathogenic biosafety

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 228, P. 115189 - 115189

Published: March 2, 2023

Language: Английский

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