Persistence of microbiological hazards in food and feed production and processing environments

EFSA Journal, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 1, 2024

Abstract Listeria monocytogenes (in the meat, fish and seafood, dairy fruit vegetable sectors), Salmonella enterica feed, egg low moisture food sectors) Cronobacter sakazakii sector) were identified as bacterial safety hazards most relevant to public health that are associated with persistence in feed processing environment (FFPE). There is a wide range of subtypes these involved FFPE. While some specific more commonly reported persistent, it currently not possible identify universal markers (i.e. genetic determinants) for this trait. Common risk factors FFPE inadequate zoning hygiene barriers; lack hygienic design equipment machines; cleaning disinfection. A well-designed environmental sampling testing programme effective strategy contamination sources detect potentially persistent hazards. The establishment barriers measures within management system, during implementation hazard analysis critical control points, key prevent and/or Once suspected plant, 'seek-and-destroy' approach frequently recommended, including intensified monitoring, introduction continuation monitoring. Successful actions triggered by L. described, well interventions direct bactericidal activity. These could be efficient if properly validated, correctly applied verified under industrial conditions. Perspectives provided performing assessment combinations sector assess relative can persistence, based on bottom-up top-down approaches. Knowledge gaps related priorities future research provided.

Language: Английский

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Expanding Our Horizons: AIE Materials in Bacterial Research

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Bacteria share a longstanding and complex relationship with humans, playing role in protecting gut health sustaining the ecosystem to cause infectious diseases antibiotic resistance. Luminogenic materials that aggregation‐induced emission (AIE) characteristics have emerged as versatile toolbox for bacterial studies through fluorescence visualization. Numerous research efforts highlight superiority of AIE this field. Recent advances are categorized into four areas: understanding interactions, antibacterial strategies, diverse applications, synergistic applications bacteria. Initial focuses on visualizing unseen bacteria progresses developing strategies involving electrostatic amphiphilic luminogens (AIEgens), various enhance affinity. progress includes using photodynamic photothermal therapies, toxicity studies, combined therapies. Diverse from environmental disinfection disease treatment, utilizing coatings, sensors, wound healing materials, etc., also provided. Finally, combining achieve enhanced outcomes explored. This review summarizes developmental trend is expected provide future directions advancing methodologies.

Language: Английский

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Potential genetic markers of biofilm formation ability by Listeria monocytogenes isolated from fresh agricultural products

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 433, P. 111118 - 111118

Published: Feb. 18, 2025

Language: Английский

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Photodynamic inactivation of Listeria monocytogenes using a natural aggregation-induced emission photosensitizer and its application in salmon preservation

LWT, Journal Year: 2024, Volume and Issue: 193, P. 115762 - 115762

Published: Jan. 16, 2024

Photodynamic sterilization has been regarded as a promising alternative to conventional antibacterial approaches. However, the photodynamic efficiency of photosensitizer is often compromised by aggregation-induced quenching effects. Photosensitizers, especially natural products, with emission (AIE) properties open new avenue for sterilization. Herein, we investigated performance and mechanism AIE-type product berberine (BBR) against Listeria monocytogenes (L. monocytogenes). The bactericidal effect was assessed through determination minimum concentration implementation anti-biofilm experiments. results showed that BBR can significantly suppress proliferation L. efficiently remove monocytogenes-derived biofilm in concentration-dependent trend. killing speculated based on bacterial morphology characterization, viability staining results, enzyme activity assays, DNA electrophoresis analysis, molecular docking. excellent anti-bacterial mediated process may account its abilities irreversibly disruption membrane, inhibition intracellular decomposition molecules. We also demonstrated embedded sodium alginate/chitosan packaging film feasible maintain freshness salmon under light illumination inhibiting proliferation.

Language: Английский

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Listeria monocytogenes: the silent assassin

Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 73(3)

Published: March 20, 2024

Listeriosis is a foodborne infection in humans caused by

Language: Английский

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Virulence expression difference to intestinal cells of different pathogenic Listeria monocytogenes contaminating sausages after simulated digestive tract

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 430, P. 111067 - 111067

Published: Jan. 13, 2025

Language: Английский

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From Dormancy to Eradication: Strategies for Controlling Bacterial Persisters in Food Settings

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 1075 - 1075

Published: March 20, 2025

Bacterial persistence, a dormant state that enables microorganisms to survive harsh conditions, is significant concern in food-industry settings, where traditional antimicrobial treatments often fail eliminate these resilient cells. This article goes beyond conventional review by compiling critical information aimed at providing practical solutions combat bacterial persisters food production environments. explores the primary mechanisms behind persister cell formation, including toxin–antitoxin systems, alarmone guanosine tetraphosphate (ppGpp), stochastic processes (in which persistence occurs as random event), and SOS response. Given serious implications for safety quality, authors also report range of physical, chemical, biological methods targeting eradicating The strategies discussed, whether applied individually or combination, offer varying levels availability applicability within industry can serve guide implementing microbial contamination control plans. While progress has been achieved, further research crucial fully understand complex underlying develop effective targeted its eradication settings. Overall, translation insights into applications aims support overcoming this persistent challenge, ensuring safer, more sustainable production.

Language: Английский

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Early detection and population dynamics of Listeria monocytogenes in naturally contaminated drains from a meat processing plant

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Listeria monocytogenes, a significant foodborne pathogen, often contaminates ready-to-eat foods through cross-contamination in food processing environments, and floor drains represent one of the most common sites persistence. Subtyping L. monocytogenes from plants for purpose source tracking is usually performed on single colony obtained after selective enrichment. This study investigates temporal variation population dynamics drains, focusing diversity impact resident microbiota. Six different meat plant were each sampled four times over period 8 weeks subjected to two-step enrichment Half Fraser Full broths. The clonal complexes (CCs) at least 20 individual isolates positive sample (460 total) determined using either GenoListeria Multiplex qPCR assay or whole genome sequencing (WGS). microbiota cultures was analyzed by 16S rRNA gene amplicon metagenomic quasimetagenomic sequencing. detected majority samples CCs identified - CC9, CC11 (ST451), CC121 CC8 with up three same dominating drains. clones CC11, had persisted facility 3-5 years. composition drain remained relatively stable time, Pseudomonas, Acinetobacter, Janthinobacterium, Chryseobacterium, Staphylococcus, Sphingomonas as commonly genera. There no apparent differences microbial genera present negative samples. highlights use techniques such quasimetagenomics monitoring controlling risk contamination environments.

Language: Английский

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Role of advanced cleaning and sanitation techniques in biofilm prevention on dairy equipment

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(3)

Published: April 22, 2025

Abstract Biofilm formation on dairy equipment is a persistent challenge in the industry, contributing to product contamination, inefficiency, and economic losses. Traditional methods such as manual cleaning basic chemical sanitation are discussed foundational approaches, followed by an in‐depth investigation of cutting‐edge technologies, including clean‐in‐place systems, high‐pressure cleaning, foam ultrasonic electrochemical dry ice blasting, robotics, nanotechnology‐based agents, enzymatic cleaners, oxidizing agents. Enhanced techniques, steam, pulsed light, acidic alkaline electrolyzed water, hydrogen peroxide vapor, microbubble technology, biodegradable biocides, highlighted for their potential achieve superior while promoting sustainability. The effectiveness, feasibility, limitations these evaluated, emphasizing role maintaining hygiene reducing biofilm‐associated risks. Additionally, challenges, compatibility, cost, regulatory compliance, addressed, along with insights into future directions innovations, automation, smart green solutions. This review provides comprehensive resource researchers, industry professionals, policymakers aiming tackle biofilm production systems enhance food safety, operational efficiency,

Language: Английский

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From Traditional Typing to Genomic Precision: Whole-Genome Sequencing of Listeria monocytogenes Isolated from Refrigerated Foods in Chile

Foods, Journal Year: 2025, Volume and Issue: 14(2), P. 290 - 290

Published: Jan. 16, 2025

Ready-to-eat (RTE) foods are the most common sources of Listeria monocytogenes transmission. Whole-genome sequencing (WGS) enhances investigation foodborne outbreaks by enabling tracking pathogen and prediction genetic traits related to virulence, stress, antimicrobial resistance, which benefit food safety management. The aim this study was evaluate efficacy WGS in typing 16 L. strains isolated from refrigerated Chile, highlighting its advantages identification improvement epidemiological surveillance safety. Using cgMLST, a cluster identified comprising 2 with zero allele differences among evaluated. Ninety-four percent isolates (15/16) were serotype 1/2b, 88% them (14/16) ST5. All shared identical virulence genes adhesion (ami, iap, lapB), stress resistance (clpCEP), invasion (aut, iapcwhA, inlAB, lpeA), toxin production (hly), intracellular regulation (prfA), only 13 exhibiting bcrBC qacJ gene, confer quaternary ammonium. pCFSAN010068_01 plasmids prevalent, insertion sequences (ISLs) composite transposons (cns) detected 87.5% strains. presence various antibiotic genes, along thermal shocks disinfectants, may provide ST5 enhanced environmental hygiene treatments used studied plant.

Language: Английский

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