This
study
utilized
Escherichia
coli
bacteriophage
MS2
as
a
surrogate
to
evaluate
the
survival
of
enteric
viruses
in
oyster
meat.
The
phage
was
propagated
its
host,
coli,
spiked
into
meat
at
concentration
8.6
x10¹⁰
PFU/ml
(10.93
log
CFU/ml)
and
stored
under
chilled
conditions
on
ice
-20°C
(frozen
storage).
Phage
titres
were
measured
using
soft
agar
overlay
method,
with
sampling
conducted
every
second
day
for
ice-stored
fifth
frozen-stored
analysis
demonstrated
that
phages
by
extension
viruses,
could
persist
up
10
days
counts
reaching
4.78
PFU/g.
In
frozen
storage,
survived
35
days,
exhibiting
4
reduction
viral
counts.
These
results
highlight
significant
during
both
storage
conditions.
Foods,
Journal Year:
2024,
Volume and Issue:
13(19), P. 3032 - 3032
Published: Sept. 24, 2024
Salmonella
typhimurium
and
Listeria
monocytogenes
are
relevant
foodborne
bacterial
pathogens
which
may
cause
serious
intoxications
infectious
diseases
in
humans.
In
this
study,
a
sensitive
immunochromatographic
analysis
(ICA)
for
the
simultaneous
detection
of
these
two
was
developed.
For
this,
test
strips
containing
zones
with
specific
monoclonal
antibodies
(MAb)
against
lipopolysaccharides
S.
L.
one
control
zone
secondary
were
designed,
double-assay
conditions
optimized
to
ensure
high
analytical
parameters.
Prussian
blue
nanoparticles
(PBNPs)
used
as
nanozyme
labels
conjugated
MAbs
perform
sandwich
format
ICA.
Peroxidase-mimic
properties
PBNPs
allowed
catalytic
amplification
colorimetric
signal
on
strips,
enhancing
assay
sensitivity.
The
limits
(LODs)
cells
2
×
102
7
103
cells/mL,
respectively.
LODs
100-fold
less
than
those
achieved
due
ICA
based
traditional
gold
label.
developed
double
approbated
bacteria
cow
milk
samples,
processed
by
simple
dilution
buffer
before
assay.
monocytogenes,
recoveries
from
86.3
±
9.8
118.2
10.5%
correlated
well
estimated
enzyme-linked
immunosorbent
reference
method.
proposed
approach
characterized
specificity:
no
cross-reactivity
other
strains
observed.
satisfies
requirements
rapid
tests:
full
cycle
sample
acquisition
result
assessment
half
an
hour.
has
application
potential
multiplex
pathogens.
This
study
utilized
Escherichia
coli
bacteriophage
MS2
as
a
surrogate
to
evaluate
the
survival
of
enteric
viruses
in
oyster
meat.
The
phage
was
propagated
its
host,
coli,
spiked
into
meat
at
concentration
8.6
x10¹⁰
PFU/ml
(10.93
log
CFU/ml)
and
stored
under
chilled
conditions
on
ice
-20°C
(frozen
storage).
Phage
titres
were
measured
using
soft
agar
overlay
method,
with
sampling
conducted
every
second
day
for
ice-stored
fifth
frozen-stored
analysis
demonstrated
that
phages
by
extension
viruses,
could
persist
up
10
days
counts
reaching
4.78
PFU/g.
In
frozen
storage,
survived
35
days,
exhibiting
4
reduction
viral
counts.
These
results
highlight
significant
during
both
storage
conditions.
