eDNA State and Medium Affect DNA Degradation Patterns in Seminatural Systems of Southern African Waterholes

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(5)

Published: Sept. 1, 2024

ABSTRACT Environmental DNA (eDNA) has evolved into a valuable asset of the ecologists' toolkit, enabling time‐ and cost‐efficient biodiversity assessments in wide variety ecosystems. Since eDNA can be isolated from broad range environmental substrates, its persistence times those media are decisive importance for drawing inferences about species presence. For first time, we characterize water sediment samples seminatural waterholes savanna system South Africa to gain better understanding decay these waterbodies. Using mesocosm experiments, tracked two different states (extracellular membrane bound), during seasons (wet dry), substrates (surface sediment). Extracellular degraded rapidly first‐order exponential fashion membrane‐bound exhibited slower decline with more intricate patterns, involving initial reduction followed by subsequent increase measured concentrations. The latter attribute cell disassociation lysis at 24–48 h after introduction environment. Higher stochasticity capture dry season highlights need higher sampling efforts natural systems which is presumably patchily distributed. Additionally, observed longer sediments than samples, due protection nucleases. We could not reveal any effects parameters on decay, emphasizing that further research needed understand dynamics waterbodies order exploit their full potential eDNA‐based bioassessments systems.

Language: Английский

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Advancements in DNA Metabarcoding Protocols for Monitoring Zooplankton in Marine and Brackish Environments

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(11), P. 2093 - 2093

Published: Nov. 19, 2024

Over the past century, numerous studies have proposed various organisms for biomonitoring of aquatic systems, but only recently has zooplankton emerged as a promising indicator water quality. The traditional identification methods, however, can be inefficient in context monitoring efforts, they are often time consuming and costly. DNA metabarcoding offers powerful alternative, providing more efficient reliable approach to monitor communities. In this review, we assess current state-of-the-art methodologies used evaluate marine brackish communities through workflow. While several emerging approaches been reported, no standardization achieved so far. extraction step gained most consensus, with widespread use commercial kits (DNeasy Blood & Tissue kit employed ca. 25% studies), though there is still significant variation selection. Additionally, 18S COI were main molecular markers (ca. 61% 54%, respectively) target region varied former. Moreover, many methodologies, particularly those processing samples, lack practical validation. Some also fail provide sufficient detail their methodology descriptions hindering reproducibility. Overall, shows great potential communities, further effort needed establish standardized practices optimize across entire methodological pipeline.

Language: Английский

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From jaguars to pathogens: simultaneous genetic detection of diverse taxa across biodiversity hotspots in the U.S. arid southwest

Conservation Genetics Resources, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Language: Английский

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EVALUATION OF ENVIRONMENTAL DNA VARIATION FOR TURBID WATER CONSIDERING DNA STATES

Japanese Journal of JSCE, Journal Year: 2024, Volume and Issue: 80(25), P. n/a - n/a

Published: Jan. 1, 2024

Language: Английский

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