Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats. DOI Creative Commons
Carola W. Meyer,

Konrad Schoettner,

Shimon Amir

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

ABSTRACT Circadian dysfunction caused by exposure to aberrant light-dark conditions is associated with abnormal alcohol consumption in humans and animal models. Changes drinking behavior have been linked alterations clock gene expression reward-related brain areas, which could be attributed either the effect of chronodisruption or alcohol. To date, however, combinatory circadian disruption on functions less understood. Moreover, despite known sex differences behavior, most research has carried out male subjects only, therefore implications for females remain unclear. address this gap, adult female rats housed under an 11h/11h cycle (LD22) standard light (LD24, 12h/ 12h light-dark) were given access intermittent protocol (IA20%) assess impact areas implicated reward: prefrontal cortex (PFC), nucleus accumbens (NAc), dorsal striatum (DS). mRNA core genes ( Bmal1 , Clock Per2 ), hormone receptors ERβ PR glutamate mGluR5 GluN2B a calcium-activated channel Kcnn2 inflammatory cytokine TNF-α ) measured at two-time points relative locomotor activity cycle. Housing LD22 did not affect intake but significantly disrupted rhythms reduced locomotion. Significant changes primarily related conditions, whereas Collectively, these results indicate that and/or distinct effects brain, may regulation drinking, addiction, and, ultimately, health.

Language: Английский

IntelliCage: the development and perspectives of a mouse- and user-friendly automated behavioral test system DOI Creative Commons

Hans‐Peter Lipp,

Sven Krackow, Emir Turkes

et al.

Frontiers in Behavioral Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: Jan. 3, 2024

IntelliCage for mice is a rodent home-cage equipped with four corner structures harboring symmetrical double panels operant conditioning at each of the two sides, either by reward (access to water) or aversion (non-painful stimuli: air-puffs, LED lights). Corner visits, nose-pokes and actual licks bottle-nipples are recorded individually using subcutaneously implanted transponders RFID identification up 16 adult housed in same home-cage. This allows recording individual in-cage activity applying reward/punishment schemes corners workflows designed on versatile graphic user interface. development had roots: (i) dissatisfaction standard approaches analyzing mouse behavior, including standardization reproducibility issues, (ii) response handling housing animal welfare (iii) increasing number models produced high work burden classic manual behavioral phenotyping single mice. (iv), studies transponder-chipped outdoor settings revealed clear genetic differences corresponding those observed testing laboratory. The latter observations were important social groups, because they contradicted traditional belief that animals must be tested under isolation prevent disturbance other group members. use IntelliCages reduced indeed amount remarkably, while its flexibility was proved wide range applications worldwide transcontinental parallel testing. Essentially, lines emerged: sophisticated analysis spontaneous behavior screening new models, hypothesis many fields neuroscience. Upcoming developments aim improved stimulus presentation learning videotracking interactions within IntelliCage. Its main advantages live context not stressfully handled experiments, restricted time can run absence humans, it increases worldwide, (iv) industrial cage permits retrospective data statistical tools even after years.

Language: Английский

Citations

17
Basic research on circadian disruption using animal and human organoid models: a review DOI
Hamideh Rahmati, Behzad Garmabi, Hamed Ghazvini

et al.

Biological Rhythm Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: Feb. 1, 2025

Language: Английский

Citations

0
Integrity of the circadian clock determines regularity of high-frequency and diurnal LFP rhythms within and between brain areas DOI Creative Commons
Paul Volkmann, A. Geiger,

Anisja Hühne-Landgraf

et al.

Molecular Psychiatry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Circadian clocks control most physiological processes of many species. We specifically wanted to investigate the influence environmental and endogenous rhythms their interplay on electrophysiological dynamics neuronal populations. Therefore, we measured local field potential (LFP) time series in wild-type Cryptochrome 1 2 deficient ( Cry1/2 −/− ) mice suprachiasmatic nucleus accumbens under regular light conditions constant darkness. Using refined descriptive statistical analyses, systematically profiled LFP activity. show that both strongly rhythmicity signals frequency components, but also shape patterns much smaller scales, as activity is significantly less at each more synchronous within between brain areas than mice. These results functional circadian are integral for non-circadian coordination ensemble dynamics.

Language: Английский

Citations

1
Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats DOI Creative Commons

Christiane Meyer,

Konrad Schoettner,

Shimon Amir

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: Nov. 18, 2024

Circadian dysfunction caused by exposure to aberrant light–dark conditions is associated with abnormal alcohol consumption in humans and animal models. Changes drinking behavior have been linked alterations clock gene expression reward-related brain areas, which could be attributed either the effect of chronodisruption or alcohol. To date, however, combinatory circadian disruption on functions less understood. Moreover, despite known sex differences behavior, most research has carried out male subjects only, therefore implications for females remain unclear. address this gap, adult female rats housed under an 11 h/11 h cycle (LD22) standard light (LD24, 12 h/12 light–dark) were given access intermittent protocol (IA20%) assess impact areas implicated reward: prefrontal cortex (PFC), nucleus accumbens (NAc), dorsal striatum (DS). mRNA core genes ( Bmal1 , Clock Per2 ), hormone receptors ERβ PR glutamate mGluR5 GluN2B a calcium-activated channel Kcnn2 inflammatory cytokine TNF-α ) measured at two-time points relative locomotor activity cycle. Housing LD22 did not affect intake but significantly disrupted rhythms reduced locomotion. Significant changes primarily related conditions, whereas Collectively, these results indicate that and/or distinct effects brain, may regulation drinking, addiction, and, ultimately, health.

Language: Английский

Citations

0
Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats. DOI Creative Commons
Carola W. Meyer,

Konrad Schoettner,

Shimon Amir

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

ABSTRACT Circadian dysfunction caused by exposure to aberrant light-dark conditions is associated with abnormal alcohol consumption in humans and animal models. Changes drinking behavior have been linked alterations clock gene expression reward-related brain areas, which could be attributed either the effect of chronodisruption or alcohol. To date, however, combinatory circadian disruption on functions less understood. Moreover, despite known sex differences behavior, most research has carried out male subjects only, therefore implications for females remain unclear. address this gap, adult female rats housed under an 11h/11h cycle (LD22) standard light (LD24, 12h/ 12h light-dark) were given access intermittent protocol (IA20%) assess impact areas implicated reward: prefrontal cortex (PFC), nucleus accumbens (NAc), dorsal striatum (DS). mRNA core genes ( Bmal1 , Clock Per2 ), hormone receptors ERβ PR glutamate mGluR5 GluN2B a calcium-activated channel Kcnn2 inflammatory cytokine TNF-α ) measured at two-time points relative locomotor activity cycle. Housing LD22 did not affect intake but significantly disrupted rhythms reduced locomotion. Significant changes primarily related conditions, whereas Collectively, these results indicate that and/or distinct effects brain, may regulation drinking, addiction, and, ultimately, health.

Language: Английский

Citations

0