Placentas From SARS‐CoV‐2 Infection During Pregnancy Exhibit Foci of Oxidative Stress and DNA Damage DOI
Guilherme de Moraes Nobrega, Eliza R. McColl, Arthur Antolini‐Tavares

et al.

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 93(1)

Published: Dec. 30, 2024

COVID-19 during pregnancy is linked to increased maternal morbidity and a higher incidence of preterm births (PTBs), yet the underlying mechanisms remain unclear. Cellular senescence, characterized by irreversible cessation cell division, critical process in placental function, its dysregulation has been implicated complications like PTB. Senescence can be induced various stressors, including oxidative stress, DNA damage, viral infections.

Language: Английский

Preeclampsia biomarkers (sFlt-1/PlGF) dynamics are not disrupted by SARS-CoV-2 infection during pregnancy in a hypertensive disorder SARS-CoV-2 vaccinated cohort DOI
Guilherme de Moraes Nobrega, Luciana Pietro,

Sarah Luiza Dariva

et al.

Pregnancy Hypertension, Journal Year: 2025, Volume and Issue: 39, P. 101196 - 101196

Published: Jan. 31, 2025

Language: Английский

Citations

0
Evaluating the safety and efficacy of nirmatrelvir-ritonavir therapy in pregnant women with COVID-19: a systematic review and meta-analysis DOI

Omar A Hassan,

Aya Abdulkarim Elbhairy,

Aya Magdy Siam

et al.

European Journal of Clinical Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Language: Английский

Citations

0
Single-nucleus transcriptional profiling of the placenta reveals the syncytiotrophoblast stress response to COVID-19 DOI Creative Commons
Rachel A. Keuls, Scott A. Ochsner, Mary B. O’Neill

et al.

American Journal of Obstetrics and Gynecology, Journal Year: 2025, Volume and Issue: 232(4), P. S160 - S175.e7

Published: April 1, 2025

COVID-19 in pregnancy is associated with placental immune activation, inflammation, and vascular malperfusion, but its impact on syncytiotrophoblast biology function unclear. This study aimed to determine the effects of maternal syncytiotrophoblasts using single-nucleus transcriptional profiling compare stress responses preeclampsia. For characterization syncytiotrophoblasts, we used RNA sequencing platform, single-cell combinatorial indexing (sci-RNA-seq3), profile villi fetal membranes from unvaccinated patients symptomatic at birth (n = 4), gestational age-matched controls a case critical second trimester delivery term 1). Clustering nuclei differential gene expression analysis was performed Seurat. Gene ontology conducted Enrichr. High-confidence target identify key transcription factor nodes governing response SARS-CoV-2 infection. Bioinformatic approaches were further dataset published preeclampsia signatures. Tissue analysis, including immunofluorescence, validate data histology for an expanded cohort placentas: 6), asymptomatic 3), 5), severe features 7). The analyzed comprised 15 cell clusters 47,889 nuclei. We identified 3 representing fusing mature overlapping distinct COVID-19. analyses indicated that following alterations syncytiotrophoblasts: (1) endoplasmic reticulum activation signaling pathways, unfolded protein integrated response; (2) regulation by CCAAT/enhancer-binding beta (CEBPB), master lineage; (3) upregulation preeclampsia-associated genes. Using complementary methods, confirmed increased levels proteins (eg, BiP, G3BP1) (spliced XBP1 mRNA), CEBPB (phosphorylation) Increased cytotrophoblast proliferation (Ki-67) also detected COVID-19, consistent trophoblast injury. Markers demonstrated similarities phenotype Maternal lineage factor, CEBPB. Similarities between provide insights into their clinical association.

Language: Английский

Citations

0
Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damage DOI Creative Commons
Guilherme de Moraes Nobrega, Ewen McColl, Arthur Antolini‐Tavares

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

ABSTRACT Problem COVID-19 during pregnancy is linked to increased maternal morbidity and a higher incidence of preterm births, yet the underlying mechanisms remain unclear. Cellular senescence, characterized by irreversible cessation cell division, critical process in placental function its dysregulation has been implicated complications like birth. Senescence can be induced various stressors, including oxidative stress, DNA damage, viral infections. Method Study In this study, we determined whether had an impact on senescence. We examined placentas from women infected with SARS-CoV-2 (n=10 term, 4 preterm) compared uninfected controls 3 preterm). The were analyzed for infection/replication (Spike Nucleocapsid proteins), markers damage (γH2AX) stress (ROS), senescence (telomere length; cycle regulators, SASP). Results While no overall differences cellular observed between positive negative groups, found secreted SASP confocal microscopy revealed localized areas cases. Conclusions These findings indicate that infection induces warranting further investigation into perinatal outcomes.

Language: Английский

Citations

0
Expression of the mucin-like glycoprotein CD24 and its ligand siglec-10 in placentas with acute and post SARS-CoV-2 infection DOI Creative Commons

Marina Seefried,

Johanna Mittelberger,

Manuela Franitza

et al.

Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 167, P. 104400 - 104400

Published: Nov. 27, 2024

Language: Английский

Citations

0
Placentas From SARS‐CoV‐2 Infection During Pregnancy Exhibit Foci of Oxidative Stress and DNA Damage DOI
Guilherme de Moraes Nobrega, Eliza R. McColl, Arthur Antolini‐Tavares

et al.

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 93(1)

Published: Dec. 30, 2024

COVID-19 during pregnancy is linked to increased maternal morbidity and a higher incidence of preterm births (PTBs), yet the underlying mechanisms remain unclear. Cellular senescence, characterized by irreversible cessation cell division, critical process in placental function, its dysregulation has been implicated complications like PTB. Senescence can be induced various stressors, including oxidative stress, DNA damage, viral infections.

Language: Английский

Citations

0