Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 28, 2024

The rheological properties of the extracellular fluid in female reproductive tract vary spatiotemporally, however, effect on behaviour epithelial cells that line is unexplored. Here, we reveal respond to elevated viscosity culture media by modulating their development and functionality enhance cilia formation coordination. Specifically, ciliation increases 4-fold beating frequency decreases 30% when are cultured at 100 mPa·s. Further, manifest a coordinated pattern can facilitate metachronal waves. At cellular level, viscous loading activates TRPV4 channel increase intracellular Ca

Language: Английский

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Deletion of the Sodium Glucose Cotransporter 1 (Sglt‐1) impairs mouse sperm movement

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(1)

Published: Jan. 1, 2024

Abstract The Sodium Glucose Cotransporter Isoform 1 (Sglt‐1) is a symporter that moves Na + and glucose into the cell. While most studies have focused on role of Sglt‐1 in small intestine kidney, little known about this transporter's expression function other tissues. We previously shown expressed mouse sperm flagellum its inhibition interferes with metabolism function. Here, we further investigated importance sperm, using knockout (Sglt‐1 KO). RNA, immunocytochemistry, uptake analysis confirmed ablation sperm. KO male mice are fertile exhibit normal counts morphology. However, null displayed significant reduction total, progressive parameters motility compared to wild type (WT) was exacerbated when were challenged swim media higher viscosity. Parameters capacitation, namely protein tyrosine phosphorylation acrosomal reaction, similar WT decrease hyperactivation. impaired observed containing as only energy substrate. Interestingly, addition pyruvate lactate partially recovered both low high viscosity media. Altogether, these results support an important for energetics function, providing capacity uptake.

Language: Английский

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pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization

Biomolecules, Journal Year: 2024, Volume and Issue: 14(6), P. 685 - 685

Published: June 12, 2024

pH homeostasis is crucial for spermatogenesis, sperm maturation, physiological function, and fertilization in mammals. HCO3− H+ are the most significant factors involved regulating male reproductive system. Multiple pH-regulating transporters ion channels localize testis, epididymis, spermatozoa, such as (solute carrier family 4 solute 26 transporters), carbonic anhydrases, H+-transport enzymes (e.g., Na+-H+ exchangers, monocarboxylate transporters, H+-ATPases, voltage-gated proton channels). Hormone-mediated signals impose an influence on production of some or NBCe1, SLC4A2, MCT4, etc. Additionally, including sperm-specific cationic Ca2+ (CatSper) K+ (SLO3) directly indirectly regulated by pH, exerting specific actions spermatozoa. The slightly alkaline testicular conducive to whereas epididymis’s low concentration acidic lumen favorable maturation storage. Spermatozoa increases substantially after being fused with seminal fluid enhance motility. In female tract, subjected increasing concentrations uterine fallopian tube, causing a rise intracellular (pHi) leading hyperpolarization plasma membranes, capacitation, hyperactivation, acrosome reaction, ultimately fertilization. regulation initiated SLC26A3, SLC26A8, NHA1, sNHE, CFTR localized proven certain be fertility. This review intends present key characteristics pHi testes, efferent duct, fluid, well associated mechanisms during journey fertilization, proposing insights into outstanding subjects future research trends.

Language: Английский

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The Known and Unknown About Female Reproductive Tract Mucus Rheological Properties

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

ABSTRACT Spermatozoa reach the fallopian tube during ovulation by traveling through female reproductive tract mucus. This non‐Newtonian viscoelastic medium facilitates spermatozoon movement to accomplish fertilization or, in some cases, blocks movement, leading infertility. While rheological properties are known affect motility with vitro models using synthetic polymers, their precise effects vivo understudied. paper reviews measurements of mucus humans and model animals, focusing on viscosity its potential effect spermatozoa. Mucus tract's different compartments is poorly understood. information this subject incomplete, most mammals appear have a decrease along tracts. Based sparse information, we hypothesize that changes tracts may guide spermatozoa eggs, novel concept could improve our understanding biology.

Language: Английский

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Relevance of Aquaporins for Gamete Function and Cryopreservation

Animals, Journal Year: 2022, Volume and Issue: 12(5), P. 573 - 573

Published: Feb. 24, 2022

The interaction between cells and the extracellular medium is of great importance, drastic changes in solute concentrations drive water movement across plasma membrane. Aquaporins (AQPs) are a family transmembrane channels that allow transport small solutes cell membranes. Different members this have been identified gametes. In sperm, they relevant to osmoadaptation after entering female reproductive tract, which crucial for sperm motility activation capacitation and, thus, their fertilizing ability. addition, during cryopreservation process, since some also permeable glycerol, one most frequently used cryoprotective agents livestock. Regarding oocytes, AQPs very important maturation but cryopreservation. Further research define exact sets present oocytes from different species needed, available literature envisages certain roles does not provide complete information on whole set AQPs. This considerable importance because, specific known compensate role non-functional members.

Language: Английский

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Cordycepin improves hyperactivation and acrosome reaction through adenosine receptors during human sperm capacitation in vitro

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 12, 2024

Sperm capacitation is a prerequisite for natural or in vitro fertilization. After capacitation, sperm become hyperactivated and undergo an acrosome reaction, which helps them penetrate the oocyte. Cordycepin, bioactive compound first isolated from Cordyceps militaris, adenosine analog with numerous physiological activities. However, its effects on remain unclear. This study aims to elucidate mechanisms of cordycepin human capacitation.

Language: Английский

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Study impact smoking on Sperm Morphology and DNA fragmentation in Iraqi male fertility

Al-Ameed Journal for Medical Research and Health Sciences, Journal Year: 2024, Volume and Issue: 2(1)

Published: July 24, 2024

Background: Male infertility is issue a prevalent, involves various factors. Sperm quality, particularly influenced by smoking, crucial for fertility. DNA fragmentation, indicating damage to strands in sperm cells, gaining importance male research, especially concerning abnormal morphology.

Language: Английский

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Iberiotoxin and clofilium regulate hyperactivation, acrosome reaction, and ion homeostasis synergistically during human sperm capacitation

Molecular Reproduction and Development, Journal Year: 2023, Volume and Issue: 90(3), P. 129 - 140

Published: Jan. 22, 2023

Abstract Potassium channels play essential roles in the regulation of male fertility. However, potassium mediating K + currents human sperm (I KSper ) remain controversial. Besides SLO3, SLO1 channel is a potential candidate for KSper. This study intends to elucidate function during capacitation. Human were treated with iberiotoxin (IbTX, specific inhibitor) and clofilium (SLO3 separately or simultaneously vitro A computer‐assisted analyzer was used assess motility. The acrosome reaction (AR) analyzed using fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin staining. Sperm protein tyrosine phosphorylation studied western blotting. Intracellular Ca 2+ , Cl − pH ion fluorescence probes. Independent inhibition IbTX decreased hyperactivation, AR, phosphorylation, accompanied by an increase [K ] i [Cl but decrease [Ca . Simultaneously lower hyperactivation AR more than independent inhibition. pronounced. suggested that may have synergic SLO3

Language: Английский

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IDIOPATHIC HYPERVISCOSITY. CLINICAL CASE

Sibirskij medicinskij vestnik, Journal Year: 2023, Volume and Issue: 7(2), P. 32 - 34

Published: Jan. 1, 2023

A case of observation and treatment a 30-year-old patient from married couple with infertility in marriage for 2 years is described. At the time consulting doctor, was revealed abnormal parameters ejaculate form hyperviscosity, asthenoteratozoospermia, an increase sperm DNA fragmentation index. The underwent combination therapy, including periodic proteolytic enzyme therapy (Longidase) 11 months, followed by spontaneous pregnancy.

Language: Английский

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The Effects of Different Liquefaction Temperatures on Semen Osmolality and Sperm Viability

Uludağ Üniversitesi Tıp Fakültesi Dergisi, Journal Year: 2022, Volume and Issue: 48(1), P. 77 - 81

Published: April 1, 2022

Semen likefaksiyonu, jel görünümlü semenin prostatta üretilen proteazların enzimatik aktivitesi ile sıvılaştığı proteolitik bir işlemdir. likefaksiyonu için üremeye yardımcı tedavi laboratuvarları arasında farklı uygulamalar benimsenmiştir. Farklı sıcaklıkların semen ozmolalitesinde yol açtığı değişikliklerin sperm canlılığı üzerindeki etkileri bilinmemektedir. Bu metodolojik çalışmanın amacı, sıcaklıklarda gerçekleştirildiğinde meydana gelebilecek olası değişimlerin canlılığına etkilerini ortaya koymaktır. Çalışmaya Üreme Yardımcı Tedavi Merkezi'ne analizi veya intrauterin inseminasyon başvuran erkek hastalar dahil edilmiştir. Toplam 15 hastadan alınan örnekleri iki gruba ayrılarak 37°C'de oda sıcaklığında inkübe Her örnek ozmolalite ölçümü takiben donma noktası depresyon ozmometresi yapılmıştır. Sperm hareketliliği Makler sayım kamarasıyla belirlenmiş ve hareket tipleri Dünya Sağlık Örgütü tarafından yayınlanan Analizi Kılavuzu’nda belirtildiği şekilde sınıflandırılmıştır. eozin-nigrozin boyamasıyla test Gruplar arasındaki fark student’s t testiyle belirlenmiştir. Sonuçlar p

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