Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs

Theriogenology, Journal Year: 2015, Volume and Issue: 85(1), P. 47 - 64

Published: Oct. 5, 2015

Language: Английский

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Mitochondria Quality Control and Male Fertility

Biology, Journal Year: 2023, Volume and Issue: 12(6), P. 827 - 827

Published: June 6, 2023

Mitochondria are pivotal to cellular homeostasis, performing vital functions such as bioenergetics, biosynthesis, and cell signalling. Proper maintenance of these processes is crucial prevent disease development ensure optimal function. Mitochondrial dynamics, including fission, fusion, biogenesis, mitophagy, apoptosis, maintain mitochondrial quality control, which essential for overall health. In male reproduction, mitochondria play a role in germ any defects can have serious consequences on fertility. Reactive oxygen species (ROS) also sperm capacitation, but excessive ROS levels trigger oxidative damage. Any imbalance between caused by non-communicable diseases or environmental factors, lead an increase stress, damage, turn affect concentration, quality, motility. Therefore, assessing functionality control gain valuable insights into infertility. sum, proper health, particularly important The assessment provide information the study management infertility may new strategies its management.

Language: Английский

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Mode of action of cryoprotectants for sperm preservation

Animal Reproduction Science, Journal Year: 2016, Volume and Issue: 169, P. 2 - 5

Published: Feb. 3, 2016

Language: Английский

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The sperm mitochondrion: Organelle of many functions

Animal Reproduction Science, Journal Year: 2018, Volume and Issue: 194, P. 71 - 80

Published: March 26, 2018

Language: Английский

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Lactate and Pyruvate Are Major Sources of Energy for Stallion Sperm with Dose Effects on Mitochondrial Function, Motility, and ROS Production

Biology of Reproduction, Journal Year: 2016, Volume and Issue: 95(2), P. 34 - 34

Published: June 22, 2016

Stallion sperm rely primarily on oxidative phosphorylation for production of ATP used in motility and metabolism. The objective the study was to identify which substrates included Biggers, Whitten, Whittingham (BWW) media are key optimal mitochondrial function through measurements parameters, oxygen consumption, cellular reactive species (ROS) production. It expected that substrates, pyruvate lactate, would support better than glycolytic substrate, glucose, due direct utilization within mitochondria. Measurements were performed after incubation modified BWW with varying concentrations pyruvate, glucose. effects duration motility, ROS production, consumption determined using a linear mixed-effects model. Duplicate ejaculates from four stallions three separate experiments determine substrate availability concentration relationship present results indicate lactate most important sources energy stallion velocity, elicit dose-dependent response. Additionally, ideal maximal function, as these operate at very high level their bioenergetic capability rate Moreover, we found addition glucose is not necessary short-term storage equine sperm, may even result reduction function. Finally, have confirmed can be dysfunction well intense activity.

Language: Английский

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Impact of Oxidative Stress on Male Reproduction in Domestic and Wild Animals

Antioxidants, Journal Year: 2021, Volume and Issue: 10(7), P. 1154 - 1154

Published: July 20, 2021

Oxidative stress occurs when the levels of reactive oxygen species (ROS) overcome antioxidant defenses organism, jeopardizing several biological functions, including reproduction. In male reproductive system, oxidative not only impairs sperm fertility but also compromises offspring health and survival, inducing damage to lipids, proteins nucleic acids. Although a clear link between disorders has been demonstrated in humans laboratory rodents, little information is available about implications impaired redox homeostasis domestic wild animals. Therefore, this review aims provide an update regarding intrinsic extrinsic factors that are associated with system their impact on performance The most recent strategies for palliating detrimental effects reviewed together potential economic ecological livestock industry biodiversity conservation.

Language: Английский

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Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa

Antioxidants, Journal Year: 2019, Volume and Issue: 8(11), P. 567 - 567

Published: Nov. 19, 2019

Redox regulation and oxidative stress have become areas of major interest in spermatology. Alteration redox homeostasis is recognized as a significant cause male factor infertility behind the damage that spermatozoa experience after freezing thawing or conservation liquid state. While for long time, was just considered an overproduction reactive oxygen species, nowadays it consequence deregulation. Many essential aspects functionality are regulated, with reversible oxidation thiols cysteine residues key proteins acting “on–off” switch controlling sperm function. However, if deregulation occurs, these may irreversible stress, leading to malfunction ultimately death spermatozoa. Stallion “professional producers” species due their intense mitochondrial activity, thus sophisticated systems control also characteristic horse. As result, combined fact embryos can easily be collected this horses good model study biology its impact on embryo.

Language: Английский

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Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen–Thawed Horse Sperm

Antioxidants, Journal Year: 2024, Volume and Issue: 13(3), P. 322 - 322

Published: March 6, 2024

Cryopreservation is a stressful process for sperm, as it associated with an increased production of reactive oxygen species (ROS). Elevated ROS levels, which create imbalance antioxidant capacity, may result in membrane lipid peroxidation (LPO), protein damage and DNA fragmentation. This study aimed to determine whether the LPO fragmentation frozen–thawed horse sperm relies upon activity, including enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) paraoxonase type 1 (PON1)); non-enzymatic capacity (Trolox-equivalent (TEAC), plasma ferric reducing (FRAP) cupric (CUPRAC)); oxidative stress index (OSI) their seminal (SP). Based on total motility integrity (SYBR14+/PI−) after thawing, ejaculates were hierarchically (p < 0.001) clustered into two groups good- (GFEs) poor-(PFEs) freezability ejaculates. (global breaks) higher 0.05) PFE group than GFE group, thawing showing positive relationship SP OSI levels production. In addition, negatively correlated activity antioxidants (PON1 TEAC). The present results indicate that vary between These differences could from variations TEAC) balance oxidant components SP.

Language: Английский

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Sublethal sperm freezing damage: Manifestations and solutions

Theriogenology, Journal Year: 2018, Volume and Issue: 118, P. 172 - 181

Published: June 11, 2018

Language: Английский

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Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media

Theriogenology, Journal Year: 2016, Volume and Issue: 86(5), P. 1382 - 1392

Published: May 6, 2016

Language: Английский

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