Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches

Reproductive BioMedicine Online, Journal Year: 2018, Volume and Issue: 37(3), P. 327 - 339

Published: Aug. 22, 2018

Language: Английский

---

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

Advanced Science, Journal Year: 2021, Volume and Issue: 8(6)

Published: Feb. 1, 2021

Abstract Cryopreservation technology has developed into a fundamental and important supporting method for biomedical applications such as cell‐based therapeutics, tissue engineering, assisted reproduction, vaccine storage. The formation, growth, recrystallization of ice crystals are the major limitations in cell/tissue/organ cryopreservation, cause fatal cryoinjury to cryopreserved biological samples. Flourishing anti‐icing materials strategies can effectively regulate suppress crystals, thus reducing damage promoting cryopreservation efficiency. This review first describes basic cryodamage mechanisms process. recent development chemical ice‐inhibition molecules, including cryoprotectant, antifreeze protein, synthetic polymer, nanomaterial, hydrogel, their summarized. advanced engineering strategies, trehalose delivery, cell encapsulation, bioinspired structure design inhibition, further discussed. Furthermore, external physical field technologies used inhibiting both cooling thawing processes systematically reviewed. Finally, current challenges future perspectives inhibition high‐efficiency proposed.

Language: Английский

---

Cryopreservation: An Overview of Principles and Cell-Specific Considerations

Cell Transplantation, Journal Year: 2021, Volume and Issue: 30

Published: Jan. 1, 2021

The origins of low-temperature tissue storage research date back to the late 1800s. Over half a century later, osmotic stress was revealed be main contributor cell death during cryopreservation. Consequently, addition cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol (GLY), ethylene glycol (EG), or propylene (PG), although toxic cells at high concentrations, identified necessary step protect against rampant In stress, cooling and thawing rates were also shown have significant influence on survival low temperature storage. general, successful preservation consists CPA (commonly 10% DMSO), alone in combination with additional permeating non-permeating agents, approximately 1ºC/min, either liquid vapor phase nitrogen. general considerations, cell-specific recommendations for hepatocytes, pancreatic islets, sperm, oocytes, stem should observed maximize yields. For example, rapid is associated better cryopreservation outcomes embryonic while slow recommended hematopoietic cells, mesenchymal cells. Yields can further maximized by implementing pre-cryo steps as: pre-incubation glucose anti-oxidants, alginate encapsulation, selecting within an optimal age range functional ability. Finally, viability assays are critical determining quality post-thaw improving efficiency current methods.

Language: Английский

---

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(8), P. 2781 - 2781

Published: April 16, 2020

Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm in different ruminant species. However, significant amount still suffers considerable cryodamage, which may affect quality and fertility. Recently, use "omics" technologies cryobiology, especially proteomics studies, has led better understanding molecular modifications induced by cryopreservation, facilitating identification freezability biomarkers certain proteins that can be added before enhance cryosurvival. This review provides an updated overview mechanisms involved are part responsible structural, functional fertility changes observed frozen-thawed sperm. Moreover, basis those factors freezing resilience species is also discussed as well aspects novel strategies developed reduce including new cryoprotectants, antioxidants, proteins, nanoparticles vitrification.

Language: Английский

---

Antioxidants and Male Fertility: From Molecular Studies to Clinical Evidence

Antioxidants, Journal Year: 2019, Volume and Issue: 8(4), P. 89 - 89

Published: April 5, 2019

Spermatozoa are physiologically exposed to reactive oxygen species (ROS) that play a pivotal role on several sperm functions through activation of different intracellular mechanisms involved in physiological such as capacitation associated-events. However, ROS overproduction depletes antioxidant system, which leads condition oxidative stress (OS). Subfertile and infertile men known present higher amount the reproductive tract causes DNA damage results lower fertility pregnancy rates. Thus, there is growing number couples seeking treatment assisted technologies (ART) due OS-related problems male partner. Interestingly, although ART can be successfully used, it also related with an increase production. This has led debate if antioxidants should proposed part attempt decrease non-physiological elevated levels ROS. rationale behind oral intake positive effects reproduction outcome only supported by few studies. In addition, unclear whether negative may arise from intake. Although some contrasting reports, consumption compounds activity appears improve parameters, motility concentration, damage, but not sufficient evidence rates live birth really after Moreover, depends type antioxidants, duration, even diagnostics man’s fertility, among other factors. Literature suggests main advantage therapy extend preservation used during ART. Herein, we discuss production its relevance focus molecular clinical evidence.

Language: Английский

---

Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

Animal Reproduction Science, Journal Year: 2021, Volume and Issue: 246, P. 106904 - 106904

Published: Dec. 3, 2021

Sperm cryopreservation is one of the most important procedures in development biotechnologies for assisted reproduction. In some farm animals, use cryopreserved sperm has so many benefits which relevance become more evident recent decades. Values post-thaw quality, however, are variable among species and within individuals same species. There no standardized methodology each stages procedure (andrological examination, semen collection, dilution, centrifugation, resuspension pellet with freezing medium, packaging, evaluation), also contributes to differences studies. Cryotolerance markers seminal plasma (SP) have been evaluated prediction ejaculate freezability. addition, previous research, there a focus on supplementing media different substances, such as enzymatic non-enzymatic antioxidants. studies, inclusion these substances led improved quality fertilizing capacity result minimizing adverse effects structure function. Another approach cryoprotectants. The aim this review article provide an update animals. main detrimental described, including negative repercussion reproductive performance. Furthermore, potential molecular biomarkers predict cryotolerance discussed, well addition that can mitigate harmful impact thawing sperm.

Language: Английский

---

Impact of Cryopreservation on Spermatozoa Freeze-Thawed Traits and Relevance OMICS to Assess Sperm Cryo-Tolerance in Farm Animals

Frontiers in Veterinary Science, Journal Year: 2021, Volume and Issue: 8

Published: Feb. 25, 2021

Sperm cryopreservation is a powerful tool for the livestock breeding program. Several technical attempts have been made to enhance efficiency of spermatozoa in different farm animal species. However, it well-recognized that mammalian are susceptible cryo-injury caused by processes. Moreover, factors leading cryo-injuries complicated, and cryo-damage mechanism has not methodically explained until now, which directly influences quality frozen–thawed spermatozoa. Currently, various OMICS technologies sperm cryo-biology conducted, particularly proteomics transcriptomics studies. It contributed while exploring molecular alterations cryopreservation, identification freezability markers specific proteins could be added semen diluents before improve cryo-survival. Therefore, understanding essential optimization current Recently, application newly-emerged study effects on becoming hotspot. This review detailed an updated overview elements involved cryo-tolerance freeze-thawed quality. While also utilizing technology assesses potential biomarkers as well accurate classification between excellent poor freezer candidate.

Language: Английский

---

Sperm freezing damage: the role of regulated cell death

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: May 18, 2024

Abstract Substantial progress in research on sperm cryopreservation has occurred since the twentieth century, especially focusing improving freezing procedures and optimizing semen extenders. However, cellular biological mechanisms of damage are still unclear, which greatly restricts promotion development cryopreservation. An essential component is occurrence cell death. Considering existence multiple types death pathways, this review discusses connections between characteristics regulated (e.g., apoptosis ferroptosis), accidental intracellular ice crystals) with explores possible future directions field.

Language: Английский

---

Mode of action of cryoprotectants for sperm preservation

Animal Reproduction Science, Journal Year: 2016, Volume and Issue: 169, P. 2 - 5

Published: Feb. 3, 2016

Language: Английский

---

Strategies to Minimize Various Stress-Related Freeze–Thaw Damages During Conventional Cryopreservation of Mammalian Spermatozoa

Biopreservation and Biobanking, Journal Year: 2019, Volume and Issue: 17(6), P. 603 - 612

Published: Aug. 20, 2019

The aim of the article is to report a review on different sperm cryopreservation techniques, various stress-related freeze-thaw damages altering structure and function during conventional cryopreservation, strategies minimize these stresses. Sperm has allowed indefinite storage successful transportation valuable germplasm from proven sites at distant locations, for genetic upgradation through implementation reproductive such as artificial insemination. Different techniques have been proposed freezing directional freezing, vitrification. Drawbacks related methods, heterogeneous ice nucleation repeated cycles front that disrupts kill cells, led emergence technique. vitrification advantageous there no crystal-induced physical sperm. However, less applicability encouraging results are only reported in human, dog, cat. In spite several drawbacks, still most widely used cryopreservation. Spermatozoa experience stresses form cold shock, osmotic stress, mainly oxidative stress ultimately reduces viability fertility. Several attempts made past all individually or combination. Membrane fluidity was increased prevent shock cryocapacitation-like changes by addition cholesterol membrane. Antifreeze proteins were added semen extender due recrystallization. Oxidative reduced either neutralizing reactive oxygen species (ROS) enzymatic, nonenzymatic, plant-based antioxidants reductants; minimizing level sources like radiation exposure, leucocytes, dead defective spermatozoa, which lead ROS production process. A novel approach reduce tension microenvironment is, partial deoxygenation process, number literatures pointed out direct link O2 with production. When compared other strategies, N2 gassing found cost-effective, comparatively easy potential large-scale frozen

Language: Английский

---