A mechanical transition from tension to buckling underlies the jigsaw puzzle shape morphogenesis of histoblasts in the Drosophila epidermis DOI Creative Commons
Annafrancesca Rigato,

Huicheng Meng,

Claire Chardès

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(6), P. e3002662 - e3002662

Published: June 13, 2024

The polygonal shape of cells in proliferating epithelia is a result the tensile forces cytoskeletal cortex and packing geometry set by cell cycle. In larval Drosophila epidermis, two populations, histoblasts epithelial cells, compete for space as they grow on limited body surface. They do so absence divisions. We report striking morphological transition during development, where change from tensed network configuration with straight outlines at level adherens junctions to highly folded morphology. apical surface shrinks while their growing fold, forming deep lobules. Volume increase accommodated basally, compensating shrinking area. resembles elastic buckling, we show that imbalance between shrinkage domain continuous growth triggers buckling. Our model supported laser dissections optical tweezer experiments together computer simulations. analysis pinpoints ability store mechanical energy much greater extent than most other types investigated far, retaining dissipate stress hours time scale. Finally, propose possible mechanism size regulation histoblast through lateral pressure driven Buckling effectively compacts plane may serve avoid physical harm these adult epidermis precursors life. work indicates nondividing compressive forces, instead tension, drive

Language: Английский

Epithelial apoptosis: A back-and-forth mechanical interplay between the dying cell and its surroundings DOI

Stéphanie Arnould,

Corinne Benassayag, Tatiana Merle

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 168, P. 1 - 12

Published: Feb. 21, 2025

Language: Английский

Citations

0
Mechanical Behavior and Indentation-Induced Injury of Soft Microtissues with Different Densification Levels DOI Creative Commons
Wenjuan Zhu, Xiaoning Han, Linhong Deng

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Language: Английский

Citations

0
Bioink formulations to ameliorate bioprinting-induced loss of cellular viability DOI
Sudipto Datta, Ankita Das,

Amit Roy Chowdhury

et al.

Biointerphases, Journal Year: 2019, Volume and Issue: 14(5)

Published: Sept. 1, 2019

Extrusion bioprinting, the most affordable and convenient bioprinting modality, is also associated with high process-induced cell deaths. Mechanical stresses on cells during pneumatic or piston extrusion generate excessive reactive oxygen species activate apoptosis, inflammatory pathways in cells. In this study, a bioink formulation augmented an antioxidant, N-acetyl cysteine (NAC) as possible solution to abrogate effect of bioprinting-associated survival losses. The NAC addition bioinks did not affect process, shape fidelity, mechanical properties constructs any large extent. However, process conducted at 0.30 MPa pressure 410 μm nozzle inner diameter 3% w/v alginate, 105 cells/ml resulted losses up 25% for MC3T3 contrast, showed significant (p < 0.01) improvement day 1 (91%), while enhancement 3 viability was still greater. It further observed that (ROS) load bioprinted approximately 1.4 times higher compared control, whereas containing reduced ROS levels comparable control samples. apoptosis inflammation markers had greater role modulating apoptosis. concluded presented approach preserve functionality would be advantageous over other contemporary methods (like alterations pressure, diameter, polymer concentration, etc.) can preserved without compromising fabrication time resolution/mechanical approach.

Language: Английский

Citations

23
Multi‐objective design optimization of dental implant geometrical parameters DOI
Hamidreza Moradi,

Roozbeh Beh Aein,

George Youssef

et al.

International Journal for Numerical Methods in Biomedical Engineering, Journal Year: 2021, Volume and Issue: 37(9)

Published: July 24, 2021

Abstract In‐silico investigations are becoming an integral part of the development novel biomedical devices, including dental implants. Using computer simulations can streamline process by tuning different geometrical and structural features, emphasizing osseointegration implant design a priori, leading to optimal designs in preparation for in‐vivo trails. This research aims elucidate interrelationship between 12 variables that holistically define shape implant. The approach achieve optimality hinged on coupling finite element analysis results with fractional factorial method. latter was used determine most influential during screening process, followed parameter optimization using response surface method, regarding four objectives, namely: bone‐implant contact area, volume trabecular bone dead cells, cortical axial displacement. resulted reducing number virtual experiments substantially decreasing computational cost without compromising accuracy solution. It found optimized values improved performance significantly. validity all models verified comparing responses simulation results. A sensitivity performed five address effect friction coefficient implant‐bone joint interaction. shown mechanical behavior would be independent higher coefficients. significance this study is demonstrated determining effective possible parameters considering their singular or interactive effects.

Language: Английский

Citations

20
2D Nanosilicate for additive manufacturing: Rheological modifier, sacrificial ink and support bath DOI Creative Commons
Satyam Rajput, Kaivalya A. Deo, Tanmay Mathur

et al.

Bioprinting, Journal Year: 2022, Volume and Issue: 25, P. e00187 - e00187

Published: Jan. 8, 2022

Language: Английский

Citations

14
Mechano- and pH-sensing convergence on Ca2+-mobilising proteins – A recipe for cancer? DOI
Maike D. Glitsch

Cell Calcium, Journal Year: 2019, Volume and Issue: 80, P. 38 - 45

Published: March 28, 2019

Language: Английский

Citations

20
LINCing Senescence and Nuclear Envelope Changes DOI

Bakhita R. M. Meqbel,

Matilde Gomes,

Amr Omer

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(11), P. 1787 - 1787

Published: May 30, 2022

The nuclear envelope (NE) has emerged as a nexus for cellular organization, signaling, and survival. Beyond its role barrier to separate the nucleoplasm from cytoplasm, NE’s in supporting maintaining myriad of other functions made it target study many processes, including senescence. nucleus undergoes dramatic changes senescence, which are driven by NE. Indeed, Lamin B1, key NE protein that is consistently downregulated become marker Other proteins have also been shown play LINC (linker nucleoskeleton cytoskeleton) complex proteins. complexes span NE, forming physical connections between cytoplasm nucleoplasm. In this way, they integrate cytoplasmic mechanical signals essential not only variety but needed cell However, addition complex, often existing nucleoplasmic or soluble isoforms. Some these now important roles DNA repair, shape regulation, all This review will focus on some highlight importance

Language: Английский

Citations

10
Extracellular Matrix Secretion Mechanically Reinforces Interlocking Interfaces DOI
Alec McCarthy, Navatha Shree Sharma,

Phil A. Holubeck

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 35(5)

Published: Nov. 29, 2022

Drawing inspiration for biomaterials from biological systems has led to many biomedical innovations. One notable bioinspired device, Velcro, consists of two substrates with interlocking ability. Generating reversibly is an area investigation, as such devices can allow modular tissue engineering, biomaterial interfaces, or friction-based coupling devices. Here, a biaxially interface generated using electrostatic flocking reported. Two electrostatically flocked are mechanically and interlocked the ability resist shearing compression forces. An initial high-throughput screen polyamide flock fibers varying diameters fiber lengths conducted elucidate roles different parameters on scaffold mechanical properties. After determining most desirable via weight scoring, polylactic acid (PLA) used emulate ideal in vitro use. PLA scaffolds populated osteoblasts interlocked. Interlocked improved cell survivorship under sustained viability proliferation. Additionally, resistance cell-seeded interfaces increased increasing extracellular matrix deposition. The introduction matrix-reinforced may serve binders act engineering enable couplers applications.

Language: Английский

Citations

10
Mapping stress inside living cells by atomic force microscopy in response to environmental stimuli DOI Creative Commons
Hongxin Wang, Han Zhang, Ryo Tamura

et al.

Science and Technology of Advanced Materials, Journal Year: 2023, Volume and Issue: 24(1)

Published: Oct. 2, 2023

The response of cells to environmental stimuli, under either physiological or pathological conditions, plays a key role in determining cell fate toward adaptive survival controlled death. efficiency such feedback mechanism is closely related the most challenging human diseases, including cancer. Since cellular responses are implemented through physical forces exerted on intracellular components, more detailed knowledge force distribution modern imaging techniques needed ensure mechanistic understanding these forces. In this work, we mapped at whole-cell scale and with submicron resolution correlate cytoskeletal structures. Furthermore, visualized dynamic mechanical adapting modulations situ. Such task was achieved by using an informatics-assisted atomic microscope (AFM) indentation technique where step Markov-chain Monte Carlo optimization search for both models used fit force-displacement curves probe geometry descriptors. We demonstrated dynamics within cytoskeleton, as well nucleoskeleton living which were subjected state modulation: myosin motor inhibition, micro-compression stimulation geometrical confinement manipulation. Our results highlight alteration prestress attenuate stimuli; involve against signal-initiated death during cancer growth metastasis; initiate migration.

Language: Английский

Citations

6
Scaling of internal organs during Drosophila embryonic development DOI Creative Commons
Prabhat Tiwari,

Hamsawardhini Rengarajan,

Timothy E. Saunders

et al.

Biophysical Journal, Journal Year: 2021, Volume and Issue: 120(19), P. 4264 - 4276

Published: June 2, 2021

Many species show a diverse range of sizes; for example, domestic dogs have large variation in body mass. Yet, the internal structure organism remains similar, i.e., system scales to size. Drosophila melanogaster has been powerful model exploring scaling mechanisms. In early embryo, gene expression boundaries scale very precisely embryo length. Later development, adult wings grow with remarkable symmetry and well animal our knowledge whether organs initially size largely unknown. Here, we utilize artificially small embryos explore how three critical organs—the heart, hindgut, ventral nerve cord (VNC)—adapt changes morphology. We find that heart Intriguingly, reduction cardiac cell length, rather than number, appears be important controlling The which is first chiral organ form, displays under large-scale smaller but shows few hallmarks within wild-type variation. Finally, VNC only weak behavior; even geometry result shifts This suggests may an intrinsic minimal length independent Overall, work can adapt Drosophila, extent they varies significantly between organs.

Language: Английский

Citations

13