Pks‐positive Escherichia coli in tumor tissue and surrounding normal mucosal tissue of colorectal cancer patients

Cancer Science, Journal Year: 2024, Volume and Issue: 115(4), P. 1184 - 1195

Published: Jan. 31, 2024

Abstract A significant association exists between the gut microbiome and colorectal carcinogenesis, as well cancer progression. It has been reported that Escherichia coli ( E. ) containing polyketide synthetase pks island contribute to carcinogenesis by producing colibactin, a polyketide‐peptide genotoxin. However, functions of + in initiation, proliferation, metastasis (CRC) remain unclear. We investigated clinical significance clarify its CRC. This study included 413 patients with Pks tumor tissue normal mucosal were quantified using droplet digital PCR. was more abundant Stages 0–I than or II–IV tissue. High abundance significantly associated shallower depth (hazard ratio [HR] = 5.0, 95% confidence interval [CI] 2.3–11.3, p < 0.001) absence lymph node (HR 3.0, CI 1.8–5.1, multivariable logistic analyses. ‐low ‐negative groups shorter CRC‐specific survival 6.4, 1.7–25.6, 0.005) relapse‐free 3.1, 1.3–7.3, 0.01) compared ‐high group. CRC prognosis. These results suggest might but not be

Language: Английский

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Contribution of pks+ Escherichia coli (E. coli) to Colon Carcinogenesis

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1111 - 1111

Published: May 30, 2024

Colorectal cancer (CRC) stands as a significant global health concern, ranking second in mortality and third frequency among cancers worldwide. While only small fraction of CRC cases can be attributed to inherited genetic mutations, the majority arise sporadically due somatic mutations. Emerging evidence reveals gut microbiota dysbiosis contributing factor, wherein polyketide synthase-positive Escherichia coli (pks+ E. coli) plays pivotal role pathogenesis. pks+ bacteria produce colibactin, genotoxic protein that causes deleterious effects on DNA within host colonocytes. In this review, we examine colon carcinogenesis, elucidating how colibactin-producer induce damage, promote genomic instability, disrupt epithelial barrier, mucosal inflammation, modulate immune responses, influence cell cycle dynamics. Collectively, these actions foster microenvironment conducive tumor initiation progression. Understanding mechanisms underlying bacteria-mediated development may pave way for mass screening, early detection tumors, therapeutic strategies such modulation, bacteria-targeted therapy, checkpoint inhibition colibactin production immunomodulatory pathways.

Language: Английский

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Intratumoural pks Escherichia coli is associated with risk of metachronous colorectal cancer and adenoma development in people with Lynch syndrome

EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105661 - 105661

Published: April 1, 2025

The adverse gut microbiome may underlie the variability in risks of colorectal cancer (CRC) and metachronous CRC people with Lynch syndrome (LS). role pks+/-Escherichia coli (pks+/-E. coli), Enterotoxigenic Bacteroides fragilis (ETBF), Fusobacterium nucleatum (Fn) CRCs adenomas LS is unknown. A total 358 cases, including 386 CRCs, 90 adenomas, 195 normal colonic mucosa DNA from Australasian Colon Cancer Family Registry were tested using multiplex TaqMan qPCR. Logistic regression was used to compare intratumoural prevalence each bacteria 1336 sporadic CRCs. Cox proportional-hazards estimated associations risk neoplasia. Pks+ E. (odds ratio [95% confidence interval] = 1.60 [1.08-2.35], P 0.017), pks-E. (3.87 [2.58-5.80], < 0.001) Fn (19.47 [13.32-28.87], significantly enriched when compared initial associated an increased (hazard 2.32 [1.29-4.17], 0.005) neoplasia (1.51 [1.02-2.23], 0.040) without pks+ coli. coli, are within suggesting possible roles development LS. Having CRC, that, if validated, might benefit screening eradication. This work funded by NHMRC Investigator grant (GNT1194896) a Australia/Cancer Council NSW co-funded (GNT2012914).

Language: Английский

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The synthesis of the novel Escherichia coli toxin—colibactin and its mechanisms of tumorigenesis of colorectal cancer

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 18, 2024

is part of the normal flora human gut and performs vital functions; however, certain strains can cause disease in host, impairing function adversely affecting overall health. The pks gene cluster

Language: Английский

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Research Progress of Intestinal Flora in Colorectal Cancer

Journal of Biosciences and Medicines, Journal Year: 2024, Volume and Issue: 12(03), P. 122 - 129

Published: Jan. 1, 2024

Colorectal cancer, as a common malignant tumor, has been increasing in incidence year by and become one of the leading causes death worldwide. Meanwhile, researchers have found close relationship between dysbiosis gut microbiota colorectal which further triggered indepth exploration role pathogenesis, diagnosis, treatment cancer. Studies shown that there are specific microbial changes cancer tissues, including enrichment or depletion certain bacterial species, may be associated with tumor growth, invasion, metastasis. Additionally, to closely linked microenvironment, immune response, chemotherapy drug metabolism, other factors. In this context, it is imperative study A comprehensive understanding interaction not only helpful revealing novel mechanisms development, but also holds promise providing new strategies targets for early individualized treatment, prevention This review aims thoroughly discuss research progress its compositional characteristics, occurrence development potential clinical applications. The goal provide references insights field.

Language: Английский

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Contribution of PKS+ Escherichia coli to Colon Carcinogenesis through the inhibition of exosomal miR-885-5p

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e37346 - e37346

Published: Sept. 1, 2024

About 90 % of all colorectal cancer (CRC) fatalities are caused by the metastatic spread primary tumors, which is closely correlated with patient survival and spreads circulating tumor cells (CTCs). The epithelial-mesenchymal transition (EMT) that characterizes CTCs associated a poor prognosis. Organotropic metastasis dictated transmission miRNAs cancer-derived exosomes. purpose this research to examine PKS + E's function. Coli in CRC metastases exosomal miR-885-5p suppression.

Language: Английский

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Insights into the Two Most Common Cancers of Primitive Gut-Derived Structures and Their Microbial Connections

Medicina, Journal Year: 2024, Volume and Issue: 60(9), P. 1515 - 1515

Published: Sept. 18, 2024

The gastrointestinal and respiratory systems are closely linked in different ways, including from the embryological, anatomical, cellular, physiological angles. highest number (and various types) of microorganisms live large intestine/colon, constitute normal microbiota healthy people. Adverse alterations or dysbiosis can lead to chronic inflammation. If this detrimental condition persists, a sequence pathological events occur, such as inflammatory bowel disease, dysplasia premalignant changes, finally, cancer. One most commonly identified bacteria both disease colon cancer is

Language: Английский

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Identification of biomarkers related to Escherichia coli infection for the diagnosis of gastrointestinal tumors applying machine learning methods

Heliyon, Journal Year: 2024, Volume and Issue: 10(23), P. e40491 - e40491

Published: Nov. 16, 2024

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