Life Sciences, Journal Year: 2022, Volume and Issue: 300, P. 120565 - 120565
Published: April 21, 2022
Language: Английский
Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)
Published: March 22, 2024
Abstract Background Cancer stem-like cell is a key barrier for therapeutic resistance and metastasis in various cancers, including breast cancer, yet the underlying mechanisms are still elusive. Through genome-wide lncRNA expression profiling, we identified that LINC00115 robustly upregulated chemoresistant cancer cells (BCSCs). Methods LncRNA microarray assay was performed to document abundance changes of lncRNAs paclitaxel (PTX)-resistant MDA-MB-231 BCSC (ALDH + ) non-BCSC − ). RNA pull-down immunoprecipitation (RIP) assays were determine binding proteins LINC00115. The clinical significance pathway examined TNBC metastatic lymph node tissues. biological function investigated through gain- loss-of-function studies. molecular mechanism explored sequencing, mass spectrometry, CRISPR/Cas9-knockout system. potential xenograft animal models. Results functions as scaffold link SETDB1 PLK3, leading enhanced methylation PLK3 at both K106 K200 drug-resistant BCSC. decreases phosphorylation HIF1α thereby increases stability. HIF1α, turn, upregulates ALKBH5 reduce m 6 A modification LINC00115, resulting attenuated degradation YTHDF2-dependent A-modified Thus, this positive feedback loop provokes phenotypes enhances chemoresistance triple-negative cancer. inhibitor TTD-IN with ASO sensitizes PTX-resistant response chemotherapy model. Correlative SETDB1, prognostic cancers. Conclusions Our findings uncover critical regulator highlight targeting strategy chemotherapeutic resistant
Language: Английский
Citations
15
Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 20, 2025
This narrative review explores the link between breast cancer and night shift work in nurses, focusing on genetic epigenetic factors. Breast disproportionately affects women globally, is increasingly recognized as a potential risk factor. Nurses who consecutive overnight shifts face elevated risks due to disruptions their circadian rhythms. Studies suggest that working six or more successive shifts, particularly over five years more, may increase risk. hypothesizes sleep-wake cycle, such changes melatonin production telomere length, could contribute susceptibility. Currently, there limited evidence support this hypothesis. However, it plausible alterations, including genes like ER HER2, heighten for nurses. These alterations involve variations DNA methylation, critical cancer-related genes. We highlight various influence increased Further research needed explore underlying mechanisms contributing factors association.
Language: Английский
Citations
1
Cell Proliferation, Journal Year: 2020, Volume and Issue: 54(2)
Published: Dec. 13, 2020
Triple-negative breast cancer (TNBC) is a type of that has higher risk distant recurrence and metastasis, leading to relatively aggressive biological behaviour poor outcome. So far, the clinical management TNBC challenging because its heterogeneity paucity specific targeted therapy. Recently, various studies have identified lot differently expressed long non-coding RNAs (lncRNAs) in TNBC. Those lncRNAs been reported play important roles multistep process tumorigenesis. Here, we review characteristics lncRNAs, present current state knowledge concerning expression, function regulation Accumulating explored potential lncRNAs-based therapeutics TNBC, including techniques genetic modification using antisense oligonucleotides, locked nucleic acid RNA nanotechnology. In review, also discuss future prospects about development lncRNA-based strategies for patients.
Language: Английский
Citations
68
Frontiers in Molecular Biosciences, Journal Year: 2022, Volume and Issue: 9
Published: May 5, 2022
Background: Necroptosis has been an alternatively identified mechanism of programmed cancer cell death, which plays a significant role in cancer. However, research about necroptosis-related long noncoding RNAs (lncRNAs) are still few. Moreover, the potentially prognostic value lncRNAs and their correlation with immune microenvironment remains unclear. The present study aimed to explore potential relationship triple-negative breast (TNBC). Methods: RNA expression matrix patients TNBC was obtained from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. Finally, 107 GSE58812, 159 TCGA, 143 GSE96058 were included. Necroptosis-related screened by Cox regression Pearson analysis genes. By LASSO analysis, nine employed, necroptosis index (CNI) established; then, we evaluated its value, clinical significance, pathways, infiltration, chemotherapeutics efficacy. Results: Based on CNI divided into high- low-CNI groups, high had worse prognosis, more lymph node metastasis, larger tumor (p < 0.05). receiver operating characteristic (ROC) showed that signature performed well. result infiltration proportion different further explained low immunogenicity, leading poor therapeutic outcomes. found differences IC50 values various chemotherapeutic drugs two might provide reference make personalized chemotherapy for them. Conclusion: novel marker could not only precisely predict survival probability but also demonstrate antitumor immunity drug sensitivity.
Language: Английский
Citations
38
Cellular and Molecular Life Sciences, Journal Year: 2022, Volume and Issue: 79(1)
Published: Jan. 1, 2022
Language: Английский
Citations
37
Annals of Translational Medicine, Journal Year: 2023, Volume and Issue: 11(2), P. 119 - 119
Published: Jan. 1, 2023
Growth arrest-specific 5 (GAS5) is a long noncoding RNA (lncRNA) that regulates cell viability. GAS5 lncRNA has been shown to decrease colorectal and breast cancer carcinogenesis. Although the function mechanisms related in development of ovarian (OC) remains unclear. The goal this study was clarify essential functions regulating OC progression its underlying mechanism.Relative levels normal tissues were identified by quantitative real-time polymerase chain reaction (qRT-PCR). regulatory effects on proliferation apoptosis SK-OV-3 cells evaluated. Moreover, bioinformatics tools used predict novel target [microRNA (miRNA)] GAS5. To explore key GAS5/miRNA-23a/WT1 loop mediating progression, we performed functional experiments dual-luciferase reporter (DLR) gene assessment.Downregulation found OC, which positively correlated with poor prognosis. In addition, lower expression accelerated migration demonstrated percentage vitro experiments. It acts as molecular sponge for miR-23a cells. Additionally, WT1 detected cells, through sponging miR-23a, regulated expression. Rescue tests enhancing outputs miR-23a-WT1 axis reversed impacts silencing OC.The GAS5/miR-23a/WT1 cascade participate OC. also decreases upregulating attenuating suggesting it could be an advantageous therapeutic intervention.
Language: Английский
Citations
13
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 274, P. 132730 - 132730
Published: June 12, 2024
Language: Английский
Citations
4
Frontiers in Chemistry, Journal Year: 2021, Volume and Issue: 9
Published: Dec. 24, 2021
Hypoxia is not only the reason of tumor metastasis but also enhances spread cancer cells from original site, which results in recurrence. Herein, we developed a self-assembled RNA hydrogel that efficiently delivered synergistic DNA CpG and short hairpin (shRNA) adjuvants, as well MnO2 loaded-photodynamic agent chlorine e6 (MnO2@Ce6), chemotherapy drug doxorubicin (DOX) into MDA-MB-231cells. The consists one tumour suppressor miRNA (miRNA-205) anti-metastatic (miRNA-182), both showed an outstanding effect synergistically abrogating tumours. would be dissociated by endogenous Dicer enzyme to release loaded therapeutic molecules, meantime induce decomposition H2O2 relieve hypoxia. As result, remarkable achieved through combined chemo-photodynamic therapy, simultaneously triggers series anti-tumor immune responses. Besides, carrier modified aptamer targeted MDA-MB-231 has advantages good biocompatibility low cytotoxicity. This strategy could implemented design any other microRNA (miRNA) carrier, with treatment methods treat human cancer, thereby overcoming limitations current therapies.
Language: Английский
Citations
24
Diagnostics, Journal Year: 2023, Volume and Issue: 13(11), P. 1949 - 1949
Published: June 2, 2023
Triple-negative breast cancer (TNBC) is a specific subtype of lacking hormone receptor expression and HER2 gene amplification. TNBC represents heterogeneous cancer, characterized by poor prognosis, high invasiveness, metastatic potential, tendency to relapse. In this review, the molecular subtypes pathological aspects triple-negative are illustrated, with particular attention biomarker characteristics TNBC, namely: regulators cell proliferation migration angiogenesis, apoptosis-regulating proteins, DNA damage response, immune checkpoints, epigenetic modifications. This paper also focuses on omics approaches exploring such as genomics identify cancer-specific mutations, epigenomics altered landscapes in cells, transcriptomics explore differential mRNA protein expression. Moreover, updated neoadjuvant treatments for mentioned, underlining role immunotherapy novel targeted agents treatment TNBC.
Language: Английский
Citations
10
Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15
Published: Feb. 11, 2025
Non-coding RNAs in Breast Cancer -Volume II (ncRNAs) represent a category of RNA that do not possess protein-coding capabilities, encompassing microRNAs (miRNAs), long non-coding (lncRNAs), and circular (circRNAs). Despite their inability to encode proteins, ncRNAs play crucial role modulating the expression various molecular targets, thereby influencing specific cellular biological processes outcomes. To date, numerous have been identified shown be dysregulated across cancer types, including breast (1). Furthermore, potential as diagnostic prognostic biomarkers, well therapeutic has thoroughly investigated (1,2). This research topic collected 12 articles (four original studies, eight reviews), which concentrated on novel discoveries or assessed recent advancements cancer.Firstly, comprehensive review by Li et al. highlights promise two significant classes RNAs, namely lncRNAs miRNAs, both biomarkers for cancer, targets innovative approaches. Chen conducted substantial impact progression, diagnosis, effectiveness neoadjuvant chemotherapy triple-negative (TNBC), highlighting varied profiles lncRNAs. They provided summary precise mechanisms through modulate gene nucleus cytoplasm, post-transcriptional affect mRNA stability translation efficiency, regulating tumor cell growth, proliferation, metastasis.ncRNAs reported involved regulation signaling pathways variety molecules genes (3). 2-Methoxyestradiol (2ME2) is primary endogenous metabolite 17β-estradiol. It exhibits diminished affinity estrogen receptor compared 17β-estradiol its derivatives, mechanism action operates independently response (4). Subramani found 2ME2 modifies pertinent miRNome TNBC cells, subsequently inhibiting critical such apoptosis, metastasis. Pyruvate kinase M2 (PKM2) serves metabolic enzyme within glycolytic pathway (5) Investigating SNPs elucidates variations individual predisposition diseases, disparities pharmacological responses, differences reactions environmental stimuli (8). The findings Qi confirmed LncRNA H19 polymorphisms are linked an increased susceptibility levels associated genetic markers can profoundly influence prognosis patients with cancer. circRNAs extensively sponges compete binding miRNA-targeted messenger (9). interaction contributes establishment sophisticated regulatory framework known competitive (ceRNA) network In this topic, Liu EIF4A3 could enhance circ_0022382, elevated circ_0022382 may activate PI3K/AKT/mTOR SLC7A11 sequestering let-7a-5p. Conversely, silencing impede proliferation migration while also promoting onset disulfidptosis cancer.Exosomes extracellular vesicles encased lipid bilayer, lacking intracellular organelles yet all components cell. Tumor cells release exosomes approximately ten times greater than normal (10). These tumor-derived mediating intercellular communication implicated phases progression Primary sensitize brain microenvironmental facilitating development metastasis (BCBM) via secretion vesicle-associated miRNAs (11). originating from tumors invasion blood-brain barrier compromising integrity microvascular endothelial Khan al.provided current literature derived promote BCBM, detailing roles intricate
Language: Английский
Citations
0