Manipulating Mg/Ca ratios in MgO-CaO-SiO2 bioactive glass for achieving accelerated osteogenic differentiation of human adipose-derived stem cells

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214189 - 214189

Published: Jan. 16, 2025

Language: Английский

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Protein Crotonylation Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells via the PI3K-AKT Pathway

Stem Cells, Journal Year: 2024, Volume and Issue: 42(7), P. 650 - 661

Published: Feb. 21, 2024

Abstract Posttranslational modifications (PTMs) are crucial regulatory mechanisms for cellular differentiation and organismal development. Acylation modification is one of the main PTMs that plays a pivotal role in regulating osteogenic mesenchymal stem cells focal point research bone tissue regeneration. However, its mechanism remains incompletely understood. This article aims to investigate impact protein crotonylation on periodontal ligament (PDLSCs) elucidate underlying mechanisms. Western blot analysis identified level acetylation, crotonylation, succinylation were significantly upregulated after induction PDLSCs. Subsequently, sodium crotonate (NaCr) was added medium acyl-CoA synthetase short-chain family member 2 (ACSS2) knocked down by short hairpin RNA plasmids regulate total crotonylation. The results indicated treatment with NaCr promoted expression differentiation-related factors PDLSCs, whereas silencing ACSS2 had opposite effect. In addition, mass spectrometry used comprehensive proteome-wide PDLSCs under differentiation. revealed related PI3K-AKT signaling pathway induction. Treatment affected activation pathway. Collectively, our study demonstrates promotes via pathway, providing novel targeting therapeutic approach

Language: Английский

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hsa_circ_0001599 promotes odontogenic differentiation of human dental pulp stem cells by increasing ITGA2 expression and stability

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 17, 2025

Dental pulp regeneration is significantly aided by human dental stem cells (hDPSCs). An increasing number of studies have demonstrated that circular RNAs (circRNAs) are crucial in the multidirectional differentiation many mesenchymal cells, but their specific functions and mechanisms remain unknown. This work aimed at elucidating molecular mechanism which hsa_circ_0001599 works hDPSCs during odontogenic differentiation. The expression tissue was determined using quantitative real-time PCR (qRT‒PCR). role its were studied a variety vivo vitro assessments. facilitated overexpression hsa_circ_0001599, activated PI3K/AKT signalling pathway vitro. In vivo, can promote formation new dentin-like structures. Mechanistically, enhanced ITGA2 sponging miR-889-3p. Furthermore, interacts with methylation reader hnRNPA2B1, promoting hnRNPA2B1 translocation from nucleus to cytoplasm mRNA stability. research revealed important Thus, engineered potential be effective therapeutic targets for repair regeneration.

Language: Английский

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Long non‐coding RNA AC018926.2 regulates palmitic acid exposure‐compromised osteogenic potential of periodontal ligament stem cells via the ITGA2/FAK/AKT pathway

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(8)

Published: Jan. 31, 2023

Although obesity has been proposed as a risk factor for periodontitis, the influence of excessive fat accumulation on development periodontitis and periodontal recovery from disease remains largely unknown. This study investigated cellular response ligament stem cells (PDLSCs) to elevated levels specific fatty acid, namely, palmitic acid (PA). The mechanism by which PA exposure compromises osteogenic potential was also explored. It found that PDLSCs abundant led decreased cell differentiation. Given long non-coding RNAs (lncRNAs) play key role in adverse environmental stimuli, we screened lncRNAs were differentially expressed following using lncRNA microarray analysis, AC018926.2 identified most sensitive PA. Next, gain/loss-of-function studies illustrated an important regulator PA-mediated differentiation PDLSCs. Mechanistically, upregulated integrin α2 (ITGA2) expression therefore activated ITGA2/FAK/AKT signalling. Further functional revealed inactivation signalling silencing ITGA2 counteracted pro-osteogenic effect induced overexpression. Moreover, results bioinformatics analysis RNA immunoprecipitation assay suggested might transcriptionally regulate binding PARP1 protein. Our data suggest may serve therapeutic target management obese patients.

Language: Английский

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Long non‐coding RNA LncTUG1 regulates favourable compression force‐induced cementocytes mineralization via PU.1/TLR4/SphK1 signalling

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(6)

Published: Feb. 6, 2024

Abstract Orthodontic tooth movement (OTM) is a highly coordinated biomechanical response to orthodontic forces with active remodelling of alveolar bone but minor root resorption. Such antiresorptive properties relate cementocyte mineralization, the mechanisms which remain largely unknown. This study used microarray analysis explore long non‐coding ribonucleic acids involved in stress‐induced mineralization. Gain‐ and loss‐of‐function experiments, including Alkaline phosphatase (ALP) activity Alizarin Red S staining, quantitative real‐time polymerase chain reaction (qRT‐PCR), Western blot, immunofluorescence analyses mineralization‐associated factors, were conducted verify taurine‐upregulated gene 1 (LncTUG1) regulation via targeting Toll‐like receptor 4 (TLR4)/SphK1 axis. The luciferase reporter assays, chromatin immunoprecipitation RNA pull‐down, immunoprecipitation, co‐localization assays performed elucidate interactions between LncTUG1, PU.1, TLR4. Our findings indicated that LncTUG1 overexpression attenuated while blocking TLR4/SphK1 axis reversed inhibitory effect on vivo also confirmed involvement signalling mineralization during OTM. Mechanistically, bound PU.1 subsequently enhanced TLR4 promotor thus transcriptionally elevated expression In conclusion, our data revealed critical role regulating PU.1/TLR4/SphK1 signalling, might provide further insights for developing novel therapeutic strategies could protect roots from resorption

Language: Английский

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Spore Oil-Functionalized Selenium Nanoparticles Protect Pancreatic Beta Cells from Palmitic Acid-Induced Apoptosis via Inhibition of Oxidative Stress-Mediated Apoptotic Pathways

Antioxidants, Journal Year: 2023, Volume and Issue: 12(4), P. 840 - 840

Published: March 30, 2023

Oxidative stress damage of pancreatic β-cells is a key link in the pathogenesis type 2 diabetes mellitus. A long-term increase free fatty acids induces reactive oxygen species (ROS) β-cells, leading to apoptosis and dysfunction β-cells. Ganoderma lucidum spore oil (GLSO) functional food complex with strong antioxidant activity, but its solubility stability are poor. In present study, GLSO-functionalized selenium nanoparticles (GLSO@SeNPs) high uniform particle size were synthesized by high-pressure homogeneous emulsification method. The aim this study was investigate protective effects GLSO@SeNPs on INS-1E rat insulinoma against palmitic-acid (PA)-induced cell death, as well underlying mechanisms. Our results showed that had good biocompatibility, they significantly inhibited PA-induced cells regulating activity related enzymes, including thioredoxin reductase (TrxR), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px). Western blot analysis reversed changes MAPK pathway protein expression levels. Thus, findings provided new theoretical basis for utilizing treatment diabetes.

Language: Английский

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Lockd Enhances Mandibular Mesenchymal Stem Cell Proliferation While Inhibiting Osteogenic Capability via Binding With SUZ12 in the Inflammatory Microenvironment

Journal Of Clinical Periodontology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

ABSTRACT Aim To investigate the role of lncRNA Lockd in mandibular mesenchymal stem cell (M‐MSC) proliferation and osteogenic capability inflammatory microenvironment, focusing on its interaction with SUZ12. Materials Methods Using lncR knockdown/overexpression models a murine periodontitis model, we explored Lockd's effects M‐MSC microenvironment. Predictions from multiple databases series rescue experiments revealed regulatory Lockd/SUZ12 signalling axis Results was found to stimulate but impair differentiation. The vitro studies suggested that activation negatively inhibited differentiation process may ultimately impact bone formation periodontitis. Mechanistically, it elucidated interacts SUZ12, core component polycomb repressive complex 2 (PRC2), affect PRC2 complex's gene expression. Conclusions boosts M‐MSCs inhibits their by interacting potentially inhibiting

Language: Английский

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LncRNA in periodontal tissue‐derived cells on osteogenic differentiation in the periodontitis field

Oral Diseases, Journal Year: 2024, Volume and Issue: 30(7), P. 4087 - 4097

Published: April 24, 2024

Abstract Objective Periodontitis can lead to the destruction of periodontal tissues and potentially tooth loss. Numerous tissue‐derived cells display osteogenic differentiation potential. The presence differentially expressed long non‐coding RNAs (lncRNAs) in these indicate their ability regulate process differentiation. We aim elucidate various lncRNA‐mediated regulatory mechanisms field periodontitis at epigenetic modification, transcriptional, post‐transcriptional levels. Subjects Methods systematically searched PubMed, Web Science, ScienceDirect databases identify relevant literature discussing role lncRNAs regulating cells. identified was subsequently summarized for comprehensive review. Results In this review, we have comprehensively discussed how provide novel perspectives understanding pathogenesis progression periodontitis. Conclusion These results pivotal as regulators cells, providing a solid basis future investigations on field.

Language: Английский

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The signaling pathways involved in non‐coding RNA regulation during osteogenic differentiation of periodontal tissue‐derived cells in the field of periodontitis

Journal of Periodontal Research, Journal Year: 2023, Volume and Issue: 59(1), P. 18 - 31

Published: Nov. 14, 2023

Abstract Periodontitis is a prevalent oral disease caused by chronic inflammation of the periodontal tissues surrounding teeth, which can lead to bone loss, tooth loosening, and even loss. This has negative impact on osteogenic differentiation capacity tissue‐derived cells. Non‐coding RNAs (ncRNAs) are class RNA molecules that do not encode proteins but regulate various physiological processes. In this review, we summarized critical signaling pathways ncRNAs modulate in cells, such as Wnt, BMP/Smad, NF‐κB, PI3‐K/Akt/mTOR pathways. comprehensive exploration ncRNA‐mediated modulation offers fresh promising insights for prospective approaches management periodontitis advancement regeneration therapies.

Language: Английский

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Long Non-coding RNA-H19 Regulates Osteogenic Differentiation via microRNA-140-5p/Wnt1 Signaling in Bone Marrow Mesenchymal Stem Cells

Indian Journal of Pharmaceutical Sciences, Journal Year: 2023, Volume and Issue: 85(4)

Published: Jan. 1, 2023

To explore the influence of long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on osteogenic differentiation in bone marrow mesenchymal stem cells. First, we used dual-luciferase reporter gene detection to verify coding-H19/miRNA-140-5p/Wnt1 regulatory targeting relationship. Then coding-H19 was overexpressed or silenced cells and then coding-H19, microRNA-140-5p Wnt1 expression were assessed using quantitative real-time polymerase chain reaction cell activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide. culture performed, formation calcium nodules via Alizarin red staining, while osteocalcin runt-related transcription factor 2 detected Western blot. Wild type RNA-H19 group wild type-Wnt1 showed significantly decreased luciferase assays (p<0.05). After RNA-H19, increased (p<0.05), expression, opposite result found In terms viability, there no significant differences between groups after (p>0.05). formation, mineralized matrix level, protein Long-non overexpression promotes microRNA-140-5p/Wnt1 signaling pathway. xxx sexy video, hijabi girl painefull sex hindi audio hd, Indo scandal bokep indian desi fucked stepbrother, com porn, ibomma english movies

Language: Английский

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