SYNCAS: Efficient CRISPR/Cas9 gene-editing in difficult to transform arthropods

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 165, P. 104068 - 104068

Published: Jan. 1, 2024

The genome editing technique CRISPR/Cas9 has led to major advancements in many research fields and this state-of-the-art tool proven its use genetic studies for various arthropods. However, most transformation protocols rely on microinjection of component into embryos, a method which is challenging species. Alternatively, injections can be performed adult females, but efficiencies very low as was shown the two-spotted spider mite, Tetranychus urticae, minute important chelicerate pest crops. In study, we explored different formulations optimize maternal injection protocol T. urticae. We observed strong synergy between branched amphipathic peptide capsules saponins, resulting significant increase knock-out efficiency, exceeding 20%. This formulation, termed SYNCAS, used urticae genes – phytoene desaturase, CYP384A1 Antennapedia also allowed develop co-CRISPR strategy facilitated generation knock-in mutants. addition, SYNCAS successfully applied white white-like western flower thrips, Frankliniella occidentalis. allows routine these species game changer other hard transform

Language: Английский

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Genome sequences of four Ixodes species expands understanding of tick evolution

BMC Biology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 21, 2025

Language: Английский

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The role of ATP-binding cassette transporters in arthropod pesticide toxicity and resistance

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101200 - 101200

Published: April 17, 2024

Language: Английский

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Response of Amblyseius swirskii to deltamethrin

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract BACKGROUND The rising demand for environmentally friendly pest control highlights the importance of understanding interaction between natural enemies and pesticides. Amblyseius swirskii , a predatory mite extensively used in biocontrol, plays crucial role managing populations agricultural systems. Integrating this with selective pesticide use within integrated management (IPM) would significantly advance may reduce residues environment produce. This study characterized susceptibility two colonies to deltamethrin, widely pesticide, assess their potential integration into IPM strategies. RESULTS Both exhibited significant tolerance deltamethrin at concentrations higher than maximum recommended field rate. Our analysis identified mutations target site both populations. commercial population also showed contribution cytochromes P450 resistant phenotype. Despite these results, semi‐field trials revealed reduction counts post‐treatment deltamethrin; various experiments were conducted understand discrepancy. CONCLUSION underscores need comprehensive evaluations impacts on biological agents optimize Understanding resistance performance dynamics is developing sustainable practices that ensure environmental resilience productivity. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

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Arising amitraz and pyrethroids resistance mutations in the ectoparasitic Varroa destructor mite in Canada

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 10, 2025

The ectoparasitic mite Varroa destructor remains a great threat for the beekeeping industry, example contributing to excessive winter colony loss in Canada. For decades, beekeepers have sequentially used registered synthetic varroacides tau-fluvalinate, coumaphos, amitraz, and flumethrin, leading risk of resistance evolution mites. In addition widespread coumaphos pyrethroids, decline amitraz efficacy has recently been reported numerous regions goals this study were assess Canadian populations evaluate presence incidence mutations previously associated with pyrethroids V. destructor. Our bioassay results confirmed amitraz-resistant mites population Alberta. These phenotypic complemented by targeted genotyping octopamine receptor gene Octβ2R which revealed mutation Y215H 90% tested apiaries local allele frequencies ranging from 5 95%. showed significant correlation across apiaries. parallel, L925I L925M voltage-gated sodium channel identified 100% 33 97%, suggesting that widespread. support notion practice relying on single treatment prolonged period can increase rates current varroacides. findings suggest need large-scale monitoring via provide timely information regulators. This will enable them make an effective management plan, including rotation available treatments suppress or at least delay populations.

Language: Английский

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Tick Control Strategies: Critical Insights into Chemical, Biological, Physical, and Integrated Approaches for Effective Hard Tick Management

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(2), P. 114 - 114

Published: Feb. 2, 2025

Ticks and tick-borne diseases significantly impact animal health, public economic productivity globally, particularly in areas where the wildlife–livestock interface complicates management. This review critically examines current control strategies, focusing on chemical, biological, physical, integrated pest management (IPM) approaches. Chemical acaricides, while effective, are increasingly challenged by resistance development environmental concerns. Biological approaches, including natural predators entomopathogenic fungi, physical interventions, such as habitat modification, provide sustainable alternatives but require further optimization. IPM stands out most promising long-term solution, integrating multiple approaches to enhance efficacy reducing risks. Emerging innovations, nanotechnology-enhanced acaricides next-generation vaccines, offer avenues for improved tick control. Addressing complex challenges of requires tailored interdisciplinary collaboration, sustained research investment both veterinary health contexts.

Language: Английский

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A nuclear receptor HR96-related gene underlies large trans-driven differences in detoxification gene expression in a generalist herbivore

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 17, 2023

The role, magnitude, and molecular nature of trans-driven expression variation underlying the upregulation detoxification genes in pesticide resistant arthropod populations has remained enigmatic. In this study, we performed quantitative trait locus (eQTL) mapping (n = 458) between a susceptible strain generalist herbivore crop pest Tetranychus urticae. We found that single trans eQTL hotspot controlled large differences subset different gene families, as well other associated with host plant use. As established by additional genetic approaches including RNAi knockdown, duplicated nuclear hormone receptor HR96-related ligand-binding domain was identified causal for strains. presence family T. urticae may enable modular control use genes, facilitating species' known rapid evolution to diverse pesticides plants.

Language: Английский

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Agricultural Pest Management: The Role of Microorganisms in Biopesticides and Soil Bioremediation

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2762 - 2762

Published: Oct. 1, 2024

Pesticide use in crops is a severe problem some countries. Each country has its legislation for use, but they differ the degree of tolerance these broadly toxic products. Several synthetic pesticides can cause air, soil, and water pollution, contaminating human food chain other living beings. In addition, them accumulate environment an indeterminate amount time. The agriculture sector must guarantee healthy with sustainable production using environmentally friendly methods. this context, biological biopesticides from microbes plants are growing green solution segment. pests attack worldwide, including weeds, insects, nematodes, microorganisms such as fungi, bacteria, viruses, causing diseases economic losses. bioproducts microorganisms, microbial (MBPs) or alone, practice due to intense research world. Mainly, baculoviruses have been used sources biomolecules secondary metabolites biopesticide use. Different methods, direct soil application, spraying techniques endotherapy, seed treatment, used. Adjuvants like surfactants, protective agents, carriers improve system different formulations. tool bioremediation environment. This review summarizes topics, focusing on origin.

Language: Английский

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Nanoparticle LDH enhances RNAi efficiency of dsRNA in piercing-sucking pests by promoting dsRNA stability and transport in plants

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 6, 2024

Language: Английский

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A novel target-site mutation (H146Q) outside the ubiquinone binding site of succinate dehydrogenase confers high levels of resistance to cyflumetofen and pyflubumide in Tetranychus urticae

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 170, P. 104127 - 104127

Published: April 22, 2024

Language: Английский

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