Malpighian tubules of Rhodnius prolixus: More than post-prandial diuresis DOI Creative Commons
Ian Orchard, Areej N. Al-Dailami, Jimena Leyria

et al.

Frontiers in Insect Science, Journal Year: 2023, Volume and Issue: 3

Published: March 30, 2023

Rhodnius prolixus , a major vector of Chagas disease, may be considered the model upon which foundations insect physiology and biochemistry were built. It is an obligate blood feeder in meal triggers growth, development reproduction. The also post-prandial diuresis to maintain osmotic homeostasis. In R. as with other insects, Malpighian tubules play critical role this diuresis, much has been learned about insects. But post-genomic era brought new insights, identifying functions quite apart from for tubules. Indeed, microarrays, transcriptomes, proteomics have revealed roles that immunity, detoxification, pesticide resistance, tolerance overall stress. This particularly relevant since gorging on creates several challenges addition balance. Xenobiotics present or toxins produced by metabolism blood; these must neutralized excreted. These processes not well described at molecular level prolixus. paper will review involvement immunity aspects tubule virtue transcriptome analysis. analysis indicates potential mount robust innate immune response, contribute antioxidant production heme detoxification.

Language: Английский

Assessment of the Entomopathogenic Potential of Fungal and Bacterial Isolates from Fall Armyworm Cadavers Against Spodoptera frugiperda Caterpillars and the Adult Boll Weevil, Anthonomus grandis DOI Creative Commons

Lidiane Maria Dos Santos Moreira,

Luciene Silva Marinho,

Robério Carlos Santos Neves

et al.

Neotropical Entomology, Journal Year: 2024, Volume and Issue: 53(4), P. 889 - 906

Published: May 7, 2024

Increased attention is being focused on the biological control of agricultural pests using microorganisms, owing to their potential as a viable substitute for chemical methods. Insect cadavers constitute source entomopathogenic microorganisms. We tested whether bacteria and fungi isolated from Spodoptera frugiperda (JE Smith) could affect its survival, development, egg-laying pattern, hatchability, well induce mortality in Anthonomus grandis Boheman adults. Enterobacter hormaechei Serratia marcescens Scopulariopsis sp. Aspergillus nomiae fall armyworm pest insects were subjected an artificial diet enriched with cells or fungal spores be tested, case S. frugiperda, only A. grandis. pathogenic affecting survival adults pupae. The fungus does not caterpillars pupae; however, due late action, moths eggs may affected. also increased adults, development early stages exposure diet, indicated by vertical spore transfer offspring low hatchability. are biocontrol agents these pests, warrant further investigation toxicological point view subsequently field tests involving formulations that improve sustainability practices.

Language: Английский

Citations

2

RNA Interference-Mediated Knockdown of Bombyx mori Haemocyte-Specific Cathepsin L (Cat L)-Like Cysteine Protease Gene Increases Bacillus thuringiensis kurstaki Toxicity and Reproduction in Insect Cadavers DOI Creative Commons

Linlin Yang,

Y. Sun,

Mei‐Ling Chang

et al.

Toxins, Journal Year: 2022, Volume and Issue: 14(6), P. 394 - 394

Published: June 8, 2022

The silkworm's Cat L-like gene, which encodes a lysosomal cathepsin cysteine protease, is thought to be part of the insect's innate immunity via an as-yet-undetermined mechanism. Assuming that primary function microbial degradation in mature phagosomes, we hypothesise suppression gene expression would increase Bacillus thuringiensis (Bt) bacteraemia and toxicity knockdown insects. Here, performed functional analysis larvae were fed mulberry leaves contaminated with commercial biopesticide formulation based on Bt kurstaki (Btk) (i.e., Dipel) investigate its role insect defence against known entomopathogen. Exposure sublethal doses Dipel resulted overexpression haemolymph 24 48 h after exposure. RNA interference (RNAi)-mediated significantly increased exposed larvae. Moreover, Btk replication was higher RNAi insects, suggesting may involved bacterial killing mechanism haemocytes. Finally, our results confirm protease antimicrobial insects suggest it could used as target insecticidal efficacy Bt-based biopesticides.

Language: Английский

Citations

11

The juvenile hormone epoxide hydrolase homolog in Penaeus vannamei plays immune-related functions DOI

Zhuoyan Liu,

Zishu Huang,

Xiaoyu Zheng

et al.

Developmental & Comparative Immunology, Journal Year: 2022, Volume and Issue: 132, P. 104410 - 104410

Published: April 6, 2022

Language: Английский

Citations

10

Rethinking the ecdysteroid source during Drosophila pupal–adult development DOI
Jack L. Scanlan, Charles Robin, Christen K. Mirth

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2022, Volume and Issue: 152, P. 103891 - 103891

Published: Dec. 5, 2022

Language: Английский

Citations

10

Malpighian tubules of Rhodnius prolixus: More than post-prandial diuresis DOI Creative Commons
Ian Orchard, Areej N. Al-Dailami, Jimena Leyria

et al.

Frontiers in Insect Science, Journal Year: 2023, Volume and Issue: 3

Published: March 30, 2023

Rhodnius prolixus , a major vector of Chagas disease, may be considered the model upon which foundations insect physiology and biochemistry were built. It is an obligate blood feeder in meal triggers growth, development reproduction. The also post-prandial diuresis to maintain osmotic homeostasis. In R. as with other insects, Malpighian tubules play critical role this diuresis, much has been learned about insects. But post-genomic era brought new insights, identifying functions quite apart from for tubules. Indeed, microarrays, transcriptomes, proteomics have revealed roles that immunity, detoxification, pesticide resistance, tolerance overall stress. This particularly relevant since gorging on creates several challenges addition balance. Xenobiotics present or toxins produced by metabolism blood; these must neutralized excreted. These processes not well described at molecular level prolixus. paper will review involvement immunity aspects tubule virtue transcriptome analysis. analysis indicates potential mount robust innate immune response, contribute antioxidant production heme detoxification.

Language: Английский

Citations

6