Co-translational Assembly Pathways of Nuclear Multiprotein Complexes Involved in the Regulation of Gene Transcription DOI Creative Commons
Andrea Bernardini, Làszlò Tora

Journal of Molecular Biology, Journal Year: 2023, Volume and Issue: 436(4), P. 168382 - 168382

Published: Dec. 5, 2023

Most factors that regulate gene transcription in eukaryotic cells are multimeric, often large, protein complexes. The understanding of the biogenesis pathways such large and heterogeneous assemblies, as well dimerization partner choice among factors, is crucial to interpret control expression programs consequent cell fate decisions. Co-translational assembly (Co-TA) thought play key roles complexes by directing complex formation during synthesis. In this review we discuss principles Co-TA with a special focus for regulatory We outline expected molecular advantages establishing co-translational interactions, pointing at available, or missing, evidence each them. hypothesize different mechanisms based on explain allocation "dilemma" paralog proteins subunits shared By taking paradigm employed three related (TFIID, SAGA ATAC), alternative strategies nuclear multiprotein widespread – yet specific use involved transcription. Ultimately, outlined series open questions which demand well-defined lines research investigate regulation rely coordinated

Language: Английский

The Effects of Codon Usage on Protein Structure and Folding DOI
McKenze J. Moss, Laura M. Chamness, Patricia L. Clark

et al.

Annual Review of Biophysics, Journal Year: 2023, Volume and Issue: 53(1), P. 87 - 108

Published: Dec. 22, 2023

The rate of protein synthesis is slower than many folding reactions and varies depending on the synonymous codons encoding sequence. Synonymous codon substitutions thus have potential to regulate cotranslational mechanisms, a growing number proteins been identified with mechanisms sensitive usage. Typically, these complex pathways kinetically stable native structures. Kinetically may fold only once over their lifetime, thus, codon-mediated regulation pioneer round can lasting impact. Supporting an important role for usage in folding, conserved patterns appear homologous gene families, hinting at selection. Despite exciting developments, there remains few experimental methods capable quantifying translation elongation rates cell, which challenges development predictive understanding how biology uses folding.

Language: Английский

Citations

17
Navigating the complexities of multi-domain protein folding DOI Creative Commons

Nandakumar Rajasekaran,

Christian Kaiser

Current Opinion in Structural Biology, Journal Year: 2024, Volume and Issue: 86, P. 102790 - 102790

Published: March 2, 2024

Proteome complexity has expanded tremendously over evolutionary time, enabling biological diversification. Much of this is achieved by combining a limited set structural units into long polypeptides. This widely used strategy poses challenges for folding the resulting multi-domain proteins. As consequence, their differs from that small single-domain proteins, which generally fold quickly and reversibly. Co-translational processes chaperone interactions are important aspects protein folding. In review, we discuss some recent experimental progress toward understanding these processes.

Language: Английский

Citations

5
Co-translational Assembly Pathways of Nuclear Multiprotein Complexes Involved in the Regulation of Gene Transcription DOI Creative Commons
Andrea Bernardini, Làszlò Tora

Journal of Molecular Biology, Journal Year: 2023, Volume and Issue: 436(4), P. 168382 - 168382

Published: Dec. 5, 2023

Most factors that regulate gene transcription in eukaryotic cells are multimeric, often large, protein complexes. The understanding of the biogenesis pathways such large and heterogeneous assemblies, as well dimerization partner choice among factors, is crucial to interpret control expression programs consequent cell fate decisions. Co-translational assembly (Co-TA) thought play key roles complexes by directing complex formation during synthesis. In this review we discuss principles Co-TA with a special focus for regulatory We outline expected molecular advantages establishing co-translational interactions, pointing at available, or missing, evidence each them. hypothesize different mechanisms based on explain allocation "dilemma" paralog proteins subunits shared By taking paradigm employed three related (TFIID, SAGA ATAC), alternative strategies nuclear multiprotein widespread – yet specific use involved transcription. Ultimately, outlined series open questions which demand well-defined lines research investigate regulation rely coordinated

Language: Английский

Citations

2