Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(21), P. 5849 - 5863

Published: Sept. 26, 2023

Whole-genome duplication has long been appreciated for its role in driving phenotypic novelty plants, often altering the way organisms interface with abiotic environment. Only recently, however, have we begun to investigate how polyploidy influences interactions of plants other species, despite biotic niche being predicted as one main determinants polyploid establishment. Nevertheless, lack information about affects diversity and composition microbial taxa that colonize whether this is genotype-dependent repeatable across natural environments. This a first step towards understanding microbiome contributes We, thus, tested immediate effect on bacterial aquatic plant Spirodela polyrhiza using four pairs diploids synthetic autotetraploids. Under controlled conditions, axenic were inoculated pond waters collected from 10 field sites broad environmental gradient. Autotetraploids hosted 4%-11% greater taxonomic phylogenetic than their diploid progenitors. Polyploidy, along inoculum source genetic lineage, collectively explained 7% total variation composition. Furthermore, broadened core microbiome, autotetraploids having 15 unique addition 55 they shared diploids. Our results show whole-genome directly leads importantly dependent ancestry generalizable over many contexts.

Language: Английский

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Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 10, 2024

Abstract Early microbial colonization has a profound impact on host physiology during different stages of ontogeny. Although several studies have focused early bacterial and succession, the composition role fungal communities are poorly known in fish. Here, we sequenced internal transcribed spacer 2 (ITS2) region fungi to profile mycobiome associated with eggs, hatchlings intestine Atlantic salmon at various freshwater marine stages. In most studied, diversity was lower than diversity. There were stage-specific phylotypes belonging ontogeny but some groups, such as Candida tropicalis , Saccharomyces cerevisiae Alternaria metachromatica Davidiella tassiana Humicola nigrescens persisted successive We observed significant changes intestinal first feeding. Prior feeding, dominated, (10 weeks post hatch) (12 became dominant subsequently. Seawater transfer resulted decrease alpha an increase abundance. also notable variations beta between farms. Overall, present study sheds light from adulthood. These novel findings will be useful future investigating host-microbiota interactions context developing better nutritional health management strategies for farming.

Language: Английский

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Effects of long-term experimental warming on phyllosphere epiphytic bacterial and fungal communities of four alpine plants

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

The effects of climatic warming on phyllosphere microbial communities remain uncertain. In this study, the long-term (>10 years) experimental epiphytic bacterial and fungal Carex alrofusca, Kobresia pygmaea, Potentilla bifurca Stipa capillacea were examined in Northern Tibet. Overall, increased α-diversity, but reduced α-diversity across four host plants. Warming altered community compositions mainly by increasing Actinobacteria, Firmicutes pathotroph-saprotroph fungi, reducing Basidiomycota symbiotroph fungi relative effect 'drift & others' process community, 'dispersal limitation' 'homogeneous selection' overall may be due to temperature, leaf morphology structure physicochemical properties, ecological processes assembly topological parameters species co-occurrence networks bacteria fungi. network vertex, clustering coefficient heterogeneity, while average path length diameter species. vertex varied among plants, which diverse responses plant height, malondialdehyde, network. Therefore, can alter alpine Such changes plants cause adverse

Language: Английский

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Parabacteroides distasonis regulates the infectivity and pathogenicity of SVCV at different water temperatures

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 17, 2024

Abstract Background Spring viremia of carp virus (SVCV) infects a wide range fish species and causes high mortality rates in aquaculture. This viral infection is characterized by seasonal outbreaks that are temperature-dependent. However, the specific mechanism behind temperature-dependent SVCV infectivity pathogenicity remains unclear. Given sensitivity composition intestinal microbiota to temperature changes, it would be interesting investigate if could play role modulating at different temperatures. Results Our study found significantly higher zebrafish occurred relatively lower temperature. Comparative analysis exposed high- low-temperature conditions revealed influenced abundance diversity zebrafish. A Parabacteroides distasonis its metabolite secondary bile acid (deoxycholic acid, DCA) was detected intestine Both colonization feeding DCA low reduced caused SVCV. An vitro assay demonstrated inhibit assembly release Notably, also showed an inhibitory effect on infectious hematopoietic necrosis virus, another Rhabdoviridae member known more Conclusions provides evidence can important factor influence zebrafish, consequently impacting The findings highlight enrichment derivative, DCA, intestines raised temperature, they possess preventing other members host fish.

Language: Английский

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Gut microbiota of two invasive fishes respond differently to temperature

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 28, 2023

Temperature variation structures the composition and diversity of gut microbiomes in ectothermic animals, key regulators host physiology, with potential benefit to or lead converse results (i.e., negative). So, significance either effect may largely depend on length time exposed extreme temperatures how rapidly microbiota can be altered by change temperature. However, temporal effects temperature have rarely been clarified. To understand this issue, we two juvenile fishes (Cyprinus carpio Micropterus salmoides), which both ranked among 100 worst invasive alien species world, increased environmental sampled at multiple points after exposure so as determine when differences these communities become detectable. Further, affects function was examined comparing predicted metagenomic profiles between treatment groups final point experiment. The C. more plastic than those M. salmoides. Specifically, were greatly within 1 week, while salmoides exhibit no significant changes. identified 10 bacterial functional pathways that temperature-dependent, none found temperature-dependent. Thus, sensitive changes their significantly changed treatment. These showed differ response change, indicate they colonization modes. Broadly, confirmed short-term fluctuations are always expected alter vertebrates facing global climate change.

Language: Английский

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