Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm, Bombyx mori DOI Creative Commons

F. Yamamoto,

Takeshi Yokoyama,

Yan Su

et al.

Journal of Developmental Biology, Journal Year: 2024, Volume and Issue: 12(4), P. 31 - 31

Published: Nov. 20, 2024

The classic model of sex determination in insects suggests that they do not have hormones and is determined a cell-autonomous manner. On the other hand, there accumulating evidence development secondary sexual traits controlled non-cell-autonomous manner through external factors. To evaluate degrees regulation trait development, we analyzed dynamics sexually dimorphic transcriptome gynandromorphic individuals mo mutant strain silkworm Bombyx mori. possesses female heterogametic sex-determination system (ZZ = male/ZW female), where master regulatory gene for femaleness, Feminizer (Fem), located W chromosome. As trait, focused on fat body, which shows remarkable differences between sexes during last instar larval stage. A comparison transcriptomes bodies male larvae identified 232 sex-differentially expressed genes (S-DEGs). proportions ZZ ZW cells constituting body were calculated according to expression level Fem. Based obtained values, each S-DEG was estimated, assuming levels proportion cells. estimated 207 out S-DEGs strongly correlated with corresponding by RNA-seq. These results suggest most regulated

Language: Английский

Identification and functional analysis of the doublesex gene in the redclaw crayfish, Cherax quadricarinatus DOI
Jianbo Zheng, Lina Cai, Yongyi Jia

et al.

Gene Expression Patterns, Journal Year: 2020, Volume and Issue: 37, P. 119129 - 119129

Published: July 24, 2020

Language: Английский

Citations

23
Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers DOI Creative Commons
Wayne B. Hunter, William M. Wintermantel

Plants, Journal Year: 2021, Volume and Issue: 10(9), P. 1782 - 1782

Published: Aug. 26, 2021

The advantages from exogenously applied RNAi biopesticides have yet to be realized in through commercialization due inconsistent activity of the dsRNA trigger, and level suppression. This has prompted research on improving delivery methods for applying exogenous into plants insects management pests pathogens. Another aspect improve is incorporation modified 2′-F pyrimidine nucleotides trigger. Modified incorporating 32–55% 2′-F- produced improved that increased insect mortality by 12–35% greater than non-modified triggers same sequence. These results were repeatable across multiple Hemiptera: Asian citrus psyllid (Diaphorina citri, Liviidae); whitefly (Bemisia tabaci, Aleyroididae); glassy-winged sharpshooter (Homalodisca vitripennis, Cicadellidae). Studies using siRNA with pyrimidines mammalian cells show they resistance degradation nucleases, plus result activity, increase concentrations binding affinity mRNA target. Successful future will able repeatability field, modifications dsRNA, such as pyrimidines, after fruit trees or crop plants, ingestion insects. Costs RNA modification decreased significantly over past few years can now compete pricing commercial chemical products.

Language: Английский

Citations

17
Doublesex is essential for masculinization but not feminization in Lygus hesperus DOI
J. Joe Hull, Chan C. Heu,

Roni J. Gross

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 166, P. 104085 - 104085

Published: Feb. 2, 2024

Language: Английский

Citations

2
Candidate target genes of the male-specific expressed Doublesex in the termite Reticulitermes speratus DOI Creative Commons

Kokuto Fujiwara,

Satoshi Miyazaki, Kiyoto Maekawa

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0299900 - e0299900

Published: March 1, 2024

Eusocial insects such as termites, ants, bees, and wasps exhibit a reproductive division of labor. The developmental regulation organ (ovaries testes) is crucial for distinguishing between sterile castes. development in regulated by sex-determination pathways. sex determination gene Doublesex (Dsx), encoding transcription factors, plays an important role this pathway. Therefore, clarifying the function Dsx sexual traits to understand social evolution eusocial insects. However, no studies have reported hemimetabolous group termites. In study, we searched binding sites candidate target genes species with available genome information first step First, focused on Reticulitermes speratus identified 101 Dsx. Using similar method, obtained 112, 39, 76 lucifugus, Coptotermes formosanus, Macrotermes natalensis, respectively. Second, compared 37 common R. lucifugus. These included several probably involved spermatogenesis longevity. only few were other two species. Finally, dsRNA injection resulted differential expression genes, including piwi-like protein B-box type zinc finger ncl-1 speratus. results provide valuable resource data future functional analyses

Language: Английский

Citations

2
Comparative Transcriptome Reveals the Potential Modulation Mechanisms of Spdsx Affecting Ovarian Development of Scylla paramamosain DOI

Haifu Wan,

Yinzhen Sheng,

Ziping Zhang

et al.

Marine Biotechnology, Journal Year: 2022, Volume and Issue: 24(6), P. 1055 - 1065

Published: Sept. 29, 2022

Language: Английский

Citations

8
Isolation of two new genes encoding heat shock protein 70 in Bemisia tabaci and analysis during thermal stress DOI
Jing Bai, Yucheng Wang, Yun‐Cai Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2021, Volume and Issue: 193, P. 933 - 940

Published: Oct. 30, 2021

Language: Английский

Citations

10
Identification and Functional Analysis of the Sex-Determiner Transformer-2 Homologue in the Freshwater Pearl Mussel, Hyriopsis cumingii DOI Creative Commons
Yayu Wang, Xiaoqiang Wang,

Jingyuan Ge

et al.

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: July 8, 2021

Transformer-2 (Tra-2) is an upstream regulatory element of the sex regulation mechanism in insects and plays a critical role formation. To understand tra-2 Hyriopsis cumingii , full-length Hctra-2 (1867 bp) was obtained from gonads, sequence alignment with other species showed that HCTRA-2 protein had highly conserved RRM domain. Phylogenetic analysis close relative to mollusks TRA-2 protein. The qRT-PCR tissue-specific expression pattern abundant testes higher than ovaries ( p < 0.01). It suggests may play potential gonadal development H. . In early development, highest on third day after fertilization increased slightly 4 months 5 months, which be related embryonic determination development. situ hybridization mRNA signals were present both male female gonads. After silencing by RNAi, levels Hcfem-1b Hcdmrt changed. speculated there certain relationship between them, important Our research will help deepen our understanding shellfish mechanisms.

Language: Английский

Citations

9
Genome‐wide dissection of sex determination genes in the highly invasive whitefly species Bemisia tabaci Q/MED DOI
Yufei Liu, Weidong Xie, Lingling Guo

et al.

Insect Molecular Biology, Journal Year: 2019, Volume and Issue: 28(4), P. 509 - 519

Published: Feb. 5, 2019

Abstract Sex determination genes are important regulators of reproduction as well the development both behavioural and morphological sex characteristics. The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is an emerging insect pest worldwide. recent release genome sequence highly invasive B. Q/MED allows us to investigate mechanisms involved in determination. combined transcriptome‐wide analyses identified 26 putatively associated with Q/MED. temporal profiles these exhibited a consistent expression pattern at all developmental stages: highest transcript levels were detected eggs (21 genes, 80.8%) lowest adults (24 92.3%). was further validated by quantitative real‐time reverse transcription PCR analysis. Alternative splicing analysis found that (1) da mle have sex‐specific isoforms adults, whereas Imp does not, (2) exon skipping common mechanism This research provides comprehensive list opportunity understand underlying globally reproduces sexually asexually.

Language: Английский

Citations

7
Alternative splicing patterns of doublesex reveal a missing link between Nix and doublesex in the sex determination cascade of Aedes albopictus DOI
Binbin Jin, Yijie Zhao,

Yunqiao Dong

et al.

Insect Science, Journal Year: 2020, Volume and Issue: 28(6), P. 1601 - 1620

Published: Nov. 12, 2020

Sexual development in insects is regulated by a complicated hierarchical cascade of sex determination. The primary signals are diverse, whereas the central nexus doublesex (dsx) gene relatively conserved within pathway. Aedes (Stegomyia) albopictus an important vector with extensive worldwide distribution. We previously reported that Ae. dsx (Aalbdsx) yields one male- (AalbdsxM ) and three female-specific isoforms (AalbdsxF1-3 ); however, spatiotemporal expression profiles mechanisms regulating sex-specific alternative splicing require further investigation. In this study, we demonstrated AalbdsxM messenger RNA (mRNA) represents default pattern when analyzed human foreskin fibroblasts HeLa cells. combined reverse transcription polymerase chain reaction immunoprecipitation using specific antibodies against tagged male-determining factor AalNix confirmed indirectly regulates pre-mRNA its splicing. During early embryo stage (0-2 4-8 h), maternal dsxF dsxM were detected both sexes; then decreased until sufficient transcripts accumulated male embryos at 20-24 h. These findings suggest or more potential enhancers can shift to presence Nix influences function unknown enhancer ultimately leads formation males. Finally, our results provide insight into regulatory mechanism mosquito.

Language: Английский

Citations

7
Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci DOI Creative Commons
Yating Liu, Jinjian Yang,

Zhijia Huo

et al.

Frontiers in Physiology, Journal Year: 2020, Volume and Issue: 11

Published: Feb. 25, 2020

The intersex (ix) gene acts in concert with doublesex (dsx) at the end of sex determination hierarchy to control somatic sexual differentiation Drosophila melanogaster. Here, we report ix homologue Bemisia tabaci (Btix) differential splicing events. Four isoforms were found B. adults, including two sex-specific transcripts (BtixF and BtixM). Knockdown Btix had no measurable effects on female morphological phenotypes but reduced expression vitellogenin resulted production significantly fewer eggs, a lower eclosion rate shorter body size progeny comparison females. These results increase our understanding genes underlying reveal potential target for RNA interference-based pest management.

Language: Английский

Citations

6