Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

Pitaya stem polysaccharide promotes wound healing by modulating macrophage polarization via single-cell RNA sequencing evidence DOI
Keying Liu, Kit‐Leong Cheong,

Yunhua He

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142653 - 142653

Published: March 1, 2025

Language: Английский

Citations

0
Integrating bioinformatics and multiple machine learning to identify mitophagy-related targets for the diagnosis and treatment of diabetic foot ulcers: evidence from transcriptome analysis and drug docking DOI Creative Commons
Hui Guo,

Kui Xiao,

Yanhua Zheng

et al.

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: July 9, 2024

Background Diabetic foot ulcers are the most common and serious complication of diabetes mellitus, high morbidity, mortality, disability which greatly diminish quality life patients impose a heavy socioeconomic burden. Thus, it is urgent to identify potential biomarkers targeted drugs for diabetic ulcers. Methods In this study, we downloaded datasets related from gene expression omnibus. Dysregulation mitophagy-related genes was identified by differential analysis weighted co-expression network analysis. Multiple machine algorithms were utilized hub genes, novel artificial neural model assisting in diagnosis constructed based on their transcriptome patterns. Finally, that can target using Enrichr platform molecular docking methods. Results 702 differentially expressed ulcers, enrichment showed these associated with mitochondria energy metabolism. Subsequently, hexokinase-2, small ribosomal subunit protein us3, l-lactate dehydrogenase A chain as multiple learning validated diagnostic performance validation cohort independent present study (The areas under roc curve 0.671, 0.870, 0.739, respectively). Next, training good, 0.924 0.840, respectively. retinoic acid estradiol promising anti-diabetic targeting hexokinase-2 (−6.6 −7.2 kcal/mol), us3 (−7.5 −8.3 (−7.6 −8.5 kcal/mol). Conclusion The chain, emphasized critical roles treatment through dimensions, providing

Language: Английский

Citations

3
Characterizing Fibroblast Heterogeneity in Diabetic Wounds Through Single-Cell RNA-Sequencing DOI Creative Commons
Helen H. Wang,

Maria Korah,

Serena L. Jing

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(11), P. 2538 - 2538

Published: Nov. 7, 2024

Diabetes mellitus is an increasingly prevalent chronic metabolic disorder characterized by physiologic hyperglycemia that, when left uncontrolled, can lead to significant complications in multiple organs. Diabetic wounds are common the general population, yet underlying mechanism of impaired healing such remains unclear. Single-cell RNA-sequencing (scRNAseq) has recently emerged as a tool study gene expression heterogeneous cell populations skin wounds. Herein, we review history scRNAseq and its application diabetic wound healing, focusing on how innovations single-cell sequencing have transformed strategies for fibroblast analysis. We summarize recent research role fibroblasts describe functional cellular heterogeneity fibroblasts. Moreover, highlight future opportunities research, with focus characterizing distinct subpopulations their lineages. Leveraging technologies explore complex biology may reveal new therapeutic targets improving ultimately alleviate clinical burden

Language: Английский

Citations

1
Loureirin B Accelerates Diabetic Wound Healing by Promoting TGFβ/Smad‐Dependent Macrophage M2 Polarization: A Concerted Analytical Approach Through Single‐Cell RNA Sequencing and Experimental Verification DOI
Weijing Fan, Yin Qu, Xin Yuan

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

ABSTRACT Diabetic wound (DW) represent a significant clinical challenge and often fail to heal effectively. Loureirin B (LB), flavonoid extracted from dragon's blood, has shown potential by influencing macrophage polarization promoting healing. However, its mechanisms efficacy in DW remain be explored. This study employed single‐cell RNA sequencing analyze the classification of cells diabetic foot ulcers identify related influenced macrophages. Molecular docking was used predict interactions LB with key proteins TGFβ/Smad signaling pathway. The effects on healing were further validated through vitro vivo experiments using model. Single‐cell analysis identified specific subtypes involved process highlighted role suggested action within In studies showed that under high glucose conditions, promoted pro‐inflammatory M1 healing‐promoting M2 ECM production fibroblasts activating TGF‐β/Smad signaling. vivo, treatment enhanced rates mice fibroblast synthesis regulates promote These findings suggest could therapeutic agent for improving healing, emphasizing need explore human subjects.

Language: Английский

Citations

1