Transcriptomic DN3 clock neuron subtypes regulate Drosophila sleep DOI Creative Commons
Dingbang Ma, Jasmine Quynh Le, Xihuimin Dai

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 3, 2025

Circadian neurons within animal brains orchestrate myriad physiological processes and behaviors, but the contribution of these to regulation sleep is not well understood. To address this deficiency, we leveraged single-cell RNA sequencing generate a comprehensive census transcriptomic cell types Drosophila clock neurons. We focused principally on enigmatic DN3s, which constitute most fly brain were previously almost completely uncharacterized. These DN3s are organized into 12 clusters with unusual gene expression features compared more well-studied further show that uncharacterized DN3 subtypes promote through G protein–coupled receptor, TrissinR . Our findings indicate an intricate behavior by highlight their remarkable diversity in functional properties.

Language: Английский

The connectome of the adult Drosophila mushroom body provides insights into function DOI Creative Commons
Feng Li, Jack Lindsey, Elizabeth C. Marin

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Language: Английский

Citations

347
Sleep and Synaptic Homeostasis: Structural Evidence in Drosophila DOI
Daniel Bushey, Giulio Tononi, Chiara Cirelli

et al.

Science, Journal Year: 2011, Volume and Issue: 332(6037), P. 1576 - 1581

Published: June 24, 2011

Flies’ need for sleep depends on how many synapses are formed while awake.

Language: Английский

Citations

345
A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability DOI Creative Commons
Matthieu Flourakis,

Elżbieta Kula-Eversole,

Alan L. Hutchison

et al.

Cell, Journal Year: 2015, Volume and Issue: 162(4), P. 836 - 848

Published: Aug. 1, 2015

Language: Английский

Citations

209
A transcriptomic taxonomy of Drosophila circadian neurons around the clock DOI Creative Commons
Dingbang Ma,

Dariusz Przybylski,

Katharine C. Abruzzi

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Jan. 13, 2021

Many different functions are regulated by circadian rhythms, including those orchestrated discrete clock neurons within animal brains. To comprehensively characterize and assign cell identity to the 75 pairs of Drosophila neurons, we optimized a single-cell RNA sequencing method assayed neuron gene expression at times day. The data identify least 17 categories with striking spatial regulation expression. Transcription factor is prominent likely contributes robust oscillation many transcripts, that encode cell-surface proteins previously shown be important for recognition synapse formation during development. other clock-regulated genes also constitute an resource future mechanistic functional studies between and/or temporal signaling circuits elsewhere in fly brain.

Language: Английский

Citations

119
The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase ofDrosophila's Clock DOI Creative Commons
Taishi Yoshii,

Corinna Wülbeck,

Hana Sehadová

et al.

Journal of Neuroscience, Journal Year: 2009, Volume and Issue: 29(8), P. 2597 - 2610

Published: Feb. 25, 2009

The neuropeptide pigment-dispersing factor (PDF) is a key transmitter in the circadian clock of Drosophila melanogaster. PDF necessary for robust activity rhythms and thought to couple oscillations neurons. However, little known about action on individual Here, we combined period –luciferase reporter system with immunolabeling proteins wild-type Pdf 01 mutants dissect effects specific subgroups Additionally, levels were elevated higher than normal using neural mutants, correlation analysis locomotor protein staining served determine periods cells. We found that has multiple neurons: In some groups neurons, was required maintaining cells, others, synchronous cycling members. Other neurons cycled high amplitude absence PDF, but affected their intrinsic speed. Sometimes shortened sometimes lengthened period. Our observations indicate crucial adjusting amplitude, period, phase different players clock. Under natural conditions may be adapting Drosophila' s varying photoperiods. Indeed, show here are not able adapt long photoperiods manner.

Language: Английский

Citations

236
Circadian Remodeling of Neuronal Circuits Involved in Rhythmic Behavior DOI Creative Commons
María Paz Fernández, Jimena Berni, M. Fernanda Ceriani

et al.

PLoS Biology, Journal Year: 2008, Volume and Issue: 6(3), P. e69 - e69

Published: March 18, 2008

Clock output pathways are central to convey timing information from the circadian clock a diversity of physiological systems, ranging cell-autonomous processes behavior. While molecular mechanisms that generate and sustain rhythmicity at cellular level well understood, it is unclear how this further structured control specific behavioral outputs. Rhythmic release pigment dispersing factor (PDF) has been proposed propagate time day core pacemaker cells downstream targets underlying rhythmic locomotor activity. Indeed, such changes in PDF intensity represent only known mechanism through which circuit could communicate with its output. Here we describe novel phenomenon involving extensive remodeling axonal terminals circuit, display higher complexity during significantly lower nighttime, both under daily cycles constant conditions. In support nature, cycling lost bona fide clockless mutants. We propose clock-controlled structural plasticity as candidate contributing transmission cells.

Language: Английский

Citations

225
Development and morphology of the clock‐gene‐expressing lateral neurons of Drosophila melanogaster DOI
Charlotte Helfrich‐Förster, Orie T. Shafer,

Corinna Wülbeck

et al.

The Journal of Comparative Neurology, Journal Year: 2006, Volume and Issue: 500(1), P. 47 - 70

Published: Nov. 10, 2006

The clock-gene-expressing lateral neurons are essential for the locomotor activity rhythm of Drosophila melanogaster. Traditionally, these divided into three groups: dorsal (LN(d)), large ventral (l-LN(v)), and small (s-LN(v)), whereby latter group consists four that express neuropeptide pigment-dispersing factor (PDF) a fifth PDF-negative neuron. So far, only l-LN(v) PDF-positive s-LN(v) have been shown to project accessory medulla, neuropil contains circadian pacemaker center in several insects. We show here other also arborize predominantly forming postsynaptic sites. Both LN(d) anatomically well suited connect medullae. Whereas may receive ipsilateral photic input from Hofbauer-Buchner eyelet, invade mainly contralateral medulla thus side. differentiate during midmetamorphosis. They do so close proximity one another s-LN(v), suggesting cell groups derive common precursors.

Language: Английский

Citations

223
Functional Analysis of Circadian Pacemaker Neurons inDrosophila melanogaster DOI Creative Commons
Dirk Rieger, Orie T. Shafer, Kenji Tomioka

et al.

Journal of Neuroscience, Journal Year: 2006, Volume and Issue: 26(9), P. 2531 - 2543

Published: March 1, 2006

The molecular mechanisms of circadian rhythms are well known, but how multiple clocks within one organism generate a structured rhythmic output remains mystery. Many animals show bimodal activity with morning (M) and evening (E) bouts. One long-standing model assumes that two mutually coupled oscillators underlie these bouts different sensitivities to light. Three groups lateral neurons (LN) three dorsal govern behavioral rhythmicity Drosophila . Recent data suggest the LN (the ventral subset small cells cells) plausible candidates for M E oscillator, respectively. We provide evidence neuronal respond differently light can be completely desynchronized from another by constant light, leading components free-run periods. As expected, long-period component started bout. However, short-period originated not exclusively peak more prominently peak. This reveals an interesting deviation original Pittendrigh Daan (1976) suggests subgroup acts as “main” oscillator controlling in

Language: Английский

Citations

215
Neuropeptides in interneurons of the insect brain DOI
Dick R. Nässel, Uwe Homberg

Cell and Tissue Research, Journal Year: 2006, Volume and Issue: 326(1), P. 1 - 24

Published: June 7, 2006

Language: Английский

Citations

193
Light and Temperature Control the Contribution of Specific DN1 Neurons to Drosophila Circadian Behavior DOI Creative Commons
Yong Zhang, Yixiao Liu,

Diana Bilodeau-Wentworth

et al.

Current Biology, Journal Year: 2010, Volume and Issue: 20(7), P. 600 - 605

Published: April 1, 2010

Language: Английский

Citations

183