Changes in the phyllosphere and rhizosphere of a cloud forest tree fern along an elevation gradient DOI Creative Commons

Mariana Vélez-Múnera,

Morena Avitia, Eria A. Rebollar

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract The diversity of plant-associated microbial communities is shaped by both host factors and the environment. Natural environmental gradients, specifically elevational ones, can serve as study systems to understand community ecosystem responses changes, however relationship between elevation not completely understood, especially in non-model such wild plants. In this paper we explored role shaping structure rhizosphere phyllosphere cloud forest tree fern Cyathea fulva. Samples phyllosphere, soil were collected from 15 individual ferns across five plots along an gradient ranging 1978 2210 meters above sea level. Physicochemical data collected, with all plots. Using 16S rRNA ITS1 amplicon sequencing, tested for differences composition bacterial fungal their potential abiotic drivers. We found that alpha decreased rhizosphere, but fungi pattern was only rhizosphere. also observed significant changes Our results suggest a close overall associated ferns. envision information will help further dynamics microbiota plants, contributing conservation necessary interactions plants ecosystems wellbeing.

Language: Английский

Temperature Requirements Can Affect the Microbial Composition Causing Sour Rot in Grapes DOI Creative Commons
Chiara Brischetto, Vittorio Rossi, Irene Salotti

et al.

Environmental Microbiology Reports, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 27, 2025

ABSTRACT Sour rot (SR) is a late‐season non‐Botrytis affecting grapevines, resulting from complex interplay of microorganisms, including non‐ Saccharomyces yeasts and acetic acid bacteria. Nonmicrobial factors contributing to disease development encompass vectors (e.g., Drosophila spp.), the presence wounds or microcracks on grape berry surfaces, environmental conditions during ripening. The microbial complexes within SR‐affected grapes exhibit variability among different bioclimates seasons, with certain microorganisms predominating under specific conditions. This study examined influence microbiome composition associated bunches, utilising data 41 locations across three distinct Italian bioclimates. We selected nine yeast two bacterial species frequently isolated sour‐rotted for analysis. growth responses these temperature were assessed by categorising them into four ecophysiological clusters. Furthermore, we analysed distribution their respective clusters results indicate that microbiomes involved in SR can vary according bioclimatic grape‐growing area. Further research required comprehend ecological requirements define niches understand geographical epidemiology, enhance management strategies.

Language: Английский

Citations

0
The effects of tree diversity and neighborhood on phyllosphere fungal communities DOI Creative Commons
Michael Köhler, G. Hahn,

Maarten Kanitz

et al.

Fungal ecology, Journal Year: 2025, Volume and Issue: 76, P. 101440 - 101440

Published: May 12, 2025

Language: Английский

Citations

0
Unravelling the microbiome of wild flowering plants: a comparative study of leaves and flowers in alpine ecosystems DOI Creative Commons

Dinesh Kumar Ramakrishnan,

Franziska Jauernegger,

Daniel Hoefle

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 19, 2024

Abstract Background While substantial research has explored rhizosphere and phyllosphere microbiomes, knowledge on flower microbiome, particularly in wild plants remains limited. This study explores into the diversity, abundance, composition of bacterial fungal communities leaves flowers flowering their natural alpine habitat, considering influence environmental factors. Methods We investigated 50 representing 22 families across seven locations Austria. Sampling sites encompassed varied soil types (carbonate/silicate) altitudes (450–2760 m). Amplicon sequencing to characterize quantitative PCR assess microbial abundance was applied, biotic abiotic factors assessed. Results Our revealed distinct flowers, with higher diversity richness (228 91 ASVs) than (163 55 ASVs). In addition, Gammaproteobacteria Alphaproteobacteria suggests niche specialization for plant compartments. Location significantly shaped both community parts. Notably, type influenced but not diversity. Altitude associated increased species flowers. Furthermore, significant effects family identity emerged within a subset families, impacting Shannon richness, Conclusion provides novel insights specific microbiome plants, highlighting adaptations local environments plant–microbe coevolution. The observed specificity indicates potential role health resilience, which is crucial predicting how microbiomes respond changing environments, ultimately aiding conservation ecosystems facing climate change pressures.

Language: Английский

Citations

2
Microbiome selection and evolution within wild and domesticated plants DOI Creative Commons
Christopher J. Barnes,

Mohammad Bahram,

Mogens Nicolaisen

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

2
Continental sampling reveals core bacterial and environmentally driven fungal leaf endophytes in Heuchera DOI
Dexcem J. Pantinople,

R. Conner,

Stephanie Sutton‐Dauber

et al.

American Journal of Botany, Journal Year: 2024, Volume and Issue: 111(11)

Published: Oct. 25, 2024

Abstract Premise Endophytic plant‐microbe interactions range from mutualistic relationships that confer important ecological and agricultural traits to neutral or quasi‐parasitic relationships. In contrast root‐associated endophytes, the role of environmental host‐related factors in acquisition leaf endophyte communities at broad spatial phylogenetic scales remains sparsely studied. We assessed endofoliar diversity test hypothesis membership these microbial is driven primarily by abiotic environment host phylogeny. Methods used a geographic coverage North America genus Heuchera L. (Saxifragaceae), representing 32 species varieties across 161 populations. Bacterial fungal were characterized using 16S ITS amplicon sequencing, respectively, standard metrics calculated. assembled predictors for collection sites, including latitude, elevation, temperature, precipitation, soil parameters. Results Assembly patterns differed between bacterial endophytes. Host phylogeny was significantly associated with bacteria, while distance best predictor community composition. Species richness consistent sites species, only fungi showing response aridity precipitation some metrics. Unlike what has been observed communities, this system microbes show no relationship pH other factors. Conclusions Overall, work improves our understanding large‐scale composition endophytes highlights relative significance driving different within microbiome.

Language: Английский

Citations

1
Host species and temperature drive beech and Scots pine phyllosphere microbiota across European forests DOI Creative Commons
Daniela Sangiorgio, Joan Cáliz, Stefania Mattana

et al.

Communications Earth & Environment, Journal Year: 2024, Volume and Issue: 5(1)

Published: Nov. 29, 2024

Tree-microbe interactions are essential for forest ecosystem functioning. Most plant–microbe research has focused on the rhizosphere, while composition of microbial communities in phyllosphere remains underexplored. Here, we use 16S rRNA gene sequencing to explore differences between beech and Scots pine phyllospheric microbiomes at European continental scale, map their functional profiles, elucidate role host trees, features, environmental factors such as climate atmospheric deposition microbiota assembly. We identified tree species associated foliar trait (specifically carbon:nitrogen ratio) primary drivers bacterial communities. characterized taxonomical epiphytic bacteria across an gradient from Fennoscandia Mediterranean area, with major changes temperature nitrogen deposition. also showed that played a crucial affecting assembly both species. This study contributes advancing our understanding shaping highlighting need broad-scale comparative studies (covering wide range traits conditions) how mediates responses global change. Phyllosphere scale is influenced by traits, well deposition, according analyses leaf microbes.

Language: Английский

Citations

1
Leaf functional traits of Daphniphyllum macropodum across different altitudes in Mao’er Mountain in Southern China DOI Creative Commons
Zhangting Chen, Qiaoyu Li,

Zhaokun Jiang

et al.

Frontiers in Forests and Global Change, Journal Year: 2024, Volume and Issue: 7

Published: July 17, 2024

Investigating functional traits among mountain species with differing altitude requirements is integral to effective conservation practices. Our study aims investigate the structural and chemical characteristics of Daphniphyllum macropodum leaves at three altitudes (1100 m, 1300 1500 m) across southern China provide insight into changes in leaf (LFT) as well plant adaptations response changing environmental conditions. Leaf include thickness (LT), area (LA), specific (SLA), tissue density (LD), respectively, while properties carbon-nitrogen-phosphorus (C:N:P) contents ratios, such C/N, C/P, N/P. findings demonstrated significant effect on both (LT, SLA, LD) aspects (N, N/P) LFT. In particular, 1100 m differed greatly, having lower SLA values than m. Observable trends included an initial increase followed by a decline rose. Notable them were LT, LD, N, N/P locations. Traits significantly higher m; C/N displayed inverse trend, their lowest occurring Furthermore, this research various degrees variation LFT, exhibiting greater fluctuation traits. Robust correlations observed certain traits, SLA. interdependency relationships between N P interconnectedness. Redundancy analysis indicated that soil factors, specifically content, exerted strongest impact At D. employed acquisition strategies; however, strategies emerged, showing shift from conservative ones.

Language: Английский

Citations

0
Coniferous Tree Species Identity and Leaf Aging Alter the Composition of Phyllosphere Communities Through Changes in Leaf Traits DOI Creative Commons
Lei Wang, Zhili Liu, Cécile Brès

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Oct. 9, 2024

Phyllosphere microorganisms are essential for plant growth and health. Although there an increasing number of studies showing that the composition phyllosphere communities varies among different species, it remains unclear whether how their bacterial fungal community predictably with traits leaf age. In this study, we used high-throughput sequencing to explore diversity in needles ages (originating from cohorts) three evergreen coniferous species (Pinus koraiensis, Picea Abies nephrolepis). Our results indicated Gammaproteobacteria (bacteria) Dothideomycetes (fungi) were dominant newly formed needles, whereas Actinobacteria Eurotiomycetes perennial needles. Tree identity needle age main factors explaining variations α (species richness communities) β (dissimilarity communities). particular, found dry matter content, mass per area, total phosphorus content emerged as key predictors microbial communities, underscoring major influence tree on through changes functional traits. Finally, interaction between also contributed significantly probably because differences or environmental conditions new depend rates resource acquisition strategies. These findings provide insights into mechanisms assembly offer a better understanding interactions during aging.

Language: Английский

Citations

0
Changes in the phyllosphere and rhizosphere of a cloud forest tree fern along an elevation gradient DOI Creative Commons

Mariana Vélez-Múnera,

Morena Avitia, Eria A. Rebollar

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract The diversity of plant-associated microbial communities is shaped by both host factors and the environment. Natural environmental gradients, specifically elevational ones, can serve as study systems to understand community ecosystem responses changes, however relationship between elevation not completely understood, especially in non-model such wild plants. In this paper we explored role shaping structure rhizosphere phyllosphere cloud forest tree fern Cyathea fulva. Samples phyllosphere, soil were collected from 15 individual ferns across five plots along an gradient ranging 1978 2210 meters above sea level. Physicochemical data collected, with all plots. Using 16S rRNA ITS1 amplicon sequencing, tested for differences composition bacterial fungal their potential abiotic drivers. We found that alpha decreased rhizosphere, but fungi pattern was only rhizosphere. also observed significant changes Our results suggest a close overall associated ferns. envision information will help further dynamics microbiota plants, contributing conservation necessary interactions plants ecosystems wellbeing.

Language: Английский

Citations

0