SUMOylation of methyltransferase‐like 3 facilitates colorectal cancer progression by promoting circ_0000677 in an m⁶A‐dependent manner

Journal of Gastroenterology and Hepatology, Journal Year: 2022, Volume and Issue: 37(4), P. 700 - 713

Published: Jan. 14, 2022

Abstract Background and Aim Colorectal cancer (CRC) is one of the major health issues in world. Circ_0000677 has been shown to be upregulated CRC with unclarified function mechanism. Methyltransferase‐like 3 (METTL3) acts as a regulator for gene expression via mechanism RNA N 6 ‐methyladenosine (m A) different types cancer, which under control SUMO1‐based SUMOylation. We aim investigate their roles progression. Methods Quantitative real‐time polymerase chain reaction Western blot were used detect expressions METTL3, circ_0000677, ATP binding cassette subfamily c member 1(ABCC1) patients' tissues cell lines. The functions ABCC1 circ_0000677 studied by manipulating level knocking down or overexpression. pull‐down immunoprecipitation assays performed identify specific target genes. biological SUMOylation METTL3 was investigated vivo xenograft mice tumor model. Results samples positively regulates proliferation drug resistance affecting expression. facilitated m A modification. regulated SUMO1‐mediated CRC. Mutation METTL3‐K459 could suppress growth regulating circ_0000677/ABCC1 axis. Conclusions Overall, our study revealed that its downstream cells, induced METTL3‐mediated modification METTL3. This work provided new strategy therapeutic treatment

Language: Английский

---

Colon cancer transcriptome

Progress in Biophysics and Molecular Biology, Journal Year: 2023, Volume and Issue: 180-181, P. 49 - 82

Published: April 13, 2023

Language: Английский

---

5-methylcytosine-mediated upregulation of circular RNA 0102913 augments malignant properties of colorectal cancer cells through a microRNA-571/Rac family small GTPase 2 axis

Gene, Journal Year: 2024, Volume and Issue: 901, P. 148162 - 148162

Published: Jan. 13, 2024

Language: Английский

---

Prognostic gene expression profile of colorectal cancer

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149433 - 149433

Published: March 1, 2025

Language: Английский

---

Deciphering the role of circular RNAs in cancer progression under hypoxic conditions

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(6)

Published: May 2, 2025

Language: Английский

---

YAP derived circ-LECRC functions as a “brake signal” to suppress hyperactivation of oncogenic YAP signalling in colorectal cancer

Cancer Letters, Journal Year: 2022, Volume and Issue: 532, P. 215589 - 215589

Published: Feb. 5, 2022

Accumulating evidence indicates that circular RNAs (circRNAs) play vital roles in tumorigenesis by modulating gene expression. However, the molecular mechanisms underlying functions of circRNAs remain largely unknown. Here, we demonstrated a Yes1 associated transcriptional regulator (YAP1)-derived circRNA, circ-LECRC (circRNA low expressed CRC), was significantly downregulated colorectal cancer (CRC). High expression positively correlated with lower TNM stage and good prognosis CRC patients. Circ-LECRC overexpression inhibited cell proliferation, migration, invasion promoted apoptosis (P < 0.05). Additionally, performed xenograft lung metastasis experiments injecting cells into nude mice to mechanistically demonstrate directly binds miR-135b-5p relieve suppression its target, Krüppel-like factor 4 (KLF4). Furthermore, found both KLF4 YAP1 hyperactivation, which downregulates downstream genes pathway, such as EGFR, MYC, BIRC5, CTGF. In summary, regulates functioning competing endogenous RNA serves "brake signal" suppress hyperactivation oncogenic YAP signalling, leading tumour growth inhibition CRC.

Language: Английский

---

Promises and Challenges of Predictive Blood Biomarkers for Locally Advanced Rectal Cancer Treated with Neoadjuvant Chemoradiotherapy

Cells, Journal Year: 2023, Volume and Issue: 12(3), P. 413 - 413

Published: Jan. 26, 2023

The treatment of locally advanced rectal cancer (LARC) requires a multimodal approach combining neoadjuvant radiotherapy or chemoradiotherapy (CRT) and surgery. Predicting tumor response to CRT can guide clinical decision making improve patient care while avoiding unnecessary toxicity morbidity. Circulating biomarkers offer both the advantage be easily accessed followed over time. In recent years, such as proteins, blood cells, nucleic acids have been investigated for their predictive value in oncology. We conducted comprehensive literature review with aim summarize status circulating predicting LARC. Forty-nine publications, which forty-seven full-text articles, one systematic review, were retrieved. These studies evaluated markers (CEA CA 19-9), inflammatory (CRP, albumin, lymphocytes), hematologic (hemoglobin thrombocytes), lipids (cell-free DNA [cfDNA], [ctDNA], microRNA [miRNA]). Post-CRT CEA levels had most consistent association response, cfDNA integrity index, MGMT promoter methylation, ERCC-1, miRNAs, miRNA-related SNPs identified potential markers. Although hold great promise, inconsistent results, low statistical power, specificity sensibility prevent them from reliably following CRT. Validation standardization methods technologies are further required confirm results.

Language: Английский

---

Advanced strategies of targeting circular RNAs as therapeutic approaches in colorectal cancer drug resistance

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155402 - 155402

Published: June 14, 2024

Colorectal cancer (CRC) stands second in terms of mortality and third among the highest prevalent kinds globally. CRC prevalence is rising moderately poorly developed regions greater economically advanced regions. Despite breakthroughs targeted therapy, resistance to chemotherapeutics remains a significant challenge long-term management CRC. Circular RNAs (circRNAs) have been involved growing therapy resistance, particularly CRC, according an increasing number studies recent years. CircRNAs are one novel subclasses non-coding RNAs, previously thought as viroid. According studies, circRNAs recommended biological markers for therapeutic targets diagnostic prognostic purposes. That notable given that expression has linked hallmarks since they responsible drug patients; thereby, chemotherapy failure. Moreover, knowledge concerning relatively unclear despite using all these techniques. Here, this study, we will go over most published work highlight critical roles development main strategies overcome improve clinical outcomes.

Language: Английский

---

Circular RNA, A Molecule with Potential Chemistry and Applications in RNA-based Cancer Therapeutics: An Insight into Recent Advances

Topics in Current Chemistry, Journal Year: 2025, Volume and Issue: 383(2)

Published: May 9, 2025

Language: Английский

---

Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer

Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(1)

Published: Nov. 8, 2023

Abstract Background Colorectal cancer (CRC) is the third frequently diagnosed with high incidence and mortality rate worldwide. Our previous report has demonstrated that circCOL1A1 (hsa_circ_0044556) functions as an oncogene in CRC, Gene Ontology (GO) analysis also revealed strong association between angiogenesis. However, mechanism of or exosomal CRC angiogenesis remains elusive. Methods Purified exosomes from cells were characterized by nanoparticle tracking analyzing, electron microscopy western blot. qRT-PCR, immunohistochemistry blot employed to test expression circCOL1A1, EIF4A3, Smad pathway angiogenic markers. Cell proliferation HUVECs was monitored CCK-8 assay. The migratory capabilities detected wound healing tube formation assay, respectively. Bioinformatics analysis, RNA immunoprecipitation (RIP), pull-down FISH assays used detect interactions among EIF4A3 Smad2/3 mRNA. vitro findings verified xenograft model. Results cell-derived promoted via recruiting EIF4A3. elevated tissues, it stimulated through directly binding stabilizing Moreover, inducing signaling vitro, accelerated tumor growth vivo. Conclusion activating signaling.

Language: Английский

---